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EC number: 929-513-6 | CAS number: 1400584-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- 2001
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- 2001
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
Test material
- Reference substance name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- EC Number:
- 929-513-6
- Cas Number:
- 1400584-86-2
- Molecular formula:
- C9H13N2O3 · Na
- IUPAC Name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- Test material form:
- solid: bulk
Constituent 1
- Radiolabelling:
- no
Study design
- Test temperature:
- 25 °C
HPLC method
- Details on study design: HPLC method:
- - HPLC equipment: Agilent HPLC system with autosampler G1313A, pump G1312A and column oven G1316A, UV/VIS detector G1314A; Software Chromeleon Vers. 6.70, Fa. Dionex
- Detection wavelength: 254 nm (calibration graph and thiourea), 210 nm (analysis of the test item and thiourea)
- Column: Material: stainless steel (Bischoff), dimensions: 250 x 4.0 mm, stationary phase: Zorbax CN, 5 µm, column temperature: 25 °C
- Mobile Phase: pH 2: 0.01 M phosphoric acid / acetonitrile 60/40 (v/v) adjusted to pH 2 with phosphoric acid
pH 9: 0.01 M phosphoric acid / acetonitrile 60/40 (v/v) adjusted to pH 9 with phosphoric acid and sodium hydroxide (isocratic elution)
- Injected volume: 10 µL
- Flow rate: 1.0 mL/min
- Injection amounts:
Calibration substances: 0.11 -18 µg; Test item: 0.51 µg; Thio urea: 0.2 µg
The calibration substances were injected in duplicate dissolved in acetonitrile and the test item was injected in duplicate dissolved in acetonitrile/water 80/20 (v/v).
Results and discussion
Adsorption coefficientopen allclose all
- Type:
- Koc
- Value:
- 0.8
- pH:
- 2
- Temp.:
- 25 °C
- Key result
- Type:
- log Koc
- Value:
- -0.09
- pH:
- 2
- Temp.:
- 25 °C
- Type:
- Koc
- Value:
- 0.3
- pH:
- 9
- Temp.:
- 25 °C
- Key result
- Type:
- log Koc
- Value:
- -0.54
- pH:
- 9
- Temp.:
- 25 °C
Results: HPLC method
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration: Refer to "Any other information on materials and methods"
- Details of fitted regression line (log k' vs. log Koc): r²: 0.9544 - 0.9558
- Average retention data for test substance: 2.410 min (pH2), 2.380 min (pH9)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- No validity criteria defined in the guideline.
- Conclusions:
- The substance has a low potential for adsorption (log Koc < 3).
- Executive summary:
The adsorption coefficient (Koc) of the test item was determined according to OECD 121 guideline and EEC-guideline 2001/59/EEC C.19. Nine calibration substances were injected into an HPLC-system under the same analytical conditions (column temperature 25 °C) as the test item. A calibration curve was created by using the measured retention times (log k'-values) and the known log Koc-values of the calibration substances for linear regression. At pH 2 and pH 9 the measured log k'-values of the test item were outside at the lower end of the calibrated log k'-range. Therefore, the log Koc values of the test item were extrapolated from the resulted calibration curves.
The resulting log Koc values (-0.09 at pH2 and -0.54 at pH9) indicate a low potential for adsorption.
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