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EC number: 600-039-9 | CAS number: 10023-48-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 - 19 Apr 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hess. Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany (18 Nov 2014)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-UV
- Details on sampling:
- - Concentrations: Control and 100 mg/L
- Sampling method: Two replicates of each sample were taken directly after preparation of the test medium (0 h) and at the end of exposure (48 h). One replicate was used for analysis and the other was used as backup.
- Sample storage conditions before analysis: At ≤ - 20 °C. The stability of the test item during freezing was demonstrated in a separate non-GLP study. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 100 mg/L test item in Elendt M4 medium was prepared by stirring with a magnetic stirrer for 30 minutes.
- Controls: Elendt M4 medium (untreated test medium)
- Evidence of undissolved material: The test medium was a clear preparation and stayed unchanged throughout the study. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Ibacon GmbH, Roßdorf, Germany
- Age of parental stock: max. 6 weeks old
- Age of daphnids at test start: < 24 h old
- Culture conditions: Daphnids are kept individually in 100 mL glass vessels containing approximately 60 mL Elendt M4 medium at a water temperature of 20 ± 2 °C and a 16 h light and 8 h dark regime to ensure similar conditions as in the experiment.
- Feeding during breeding: The parental daphnids are fed ad libitum with unicellular green algae (Pseudokirchneriella subcapitata) three times per week.
- Feeding during test: No
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Parental daphnids are used for reproduction until they are about 6 weeks old. Thereafter, they are replaced by neonates.
Offspring are removed from the vessels at least twice per week. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 225 mg CaCO3/L (reconstituted water)
- Test temperature:
- 19.6 - 20.0 °C
- pH:
- 0 h: 7.82 (control), 7.03 (treatment)
48 h: 7.76 (control), 7.33 (treatment) - Dissolved oxygen:
- 0 h: 8.76 mg/L O2 (control), 8.70 mg/L O2 (treatment)
48 h: 8.44 ,g/L O2 (control), 8.37 mg/L O2 (treatment) - Conductivity:
- 652 µS/cm (reconstituted water)
- Nominal and measured concentrations:
- Control and 100 mg/L (nominal)
< LOD and 96.04 mg/L (0 h)/ 96.29 mg/L (48 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 mL glass test tubes containing at least 20 mL test medium.
- Type: Open vessels
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 4 mL medium per daphnid
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (Elendt M4 medium) was prepared by dissolving medium components in fully demineralized water (Milli-Q Water Purification System).
- Conductivity: 652 µS/cm
- Culture medium different from test medium: Culture medium same as test medium
- Intervals of water quality measurement: At test start (0 h) and at test end (48 h)
OTHER TEST CONDITIONS
- Adjustment of pH: The pH of the test medium was not adjusted.
- Photoperiod: 16 h light and 8 h dark
- Light intensity: 779 lux (mean at 0 h) and 743 lux (mean at 48 h)
EFFECT PARAMETERS MEASURED:
- Mobility: after 24 and 48 h
RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: Yes, in a static pre-test no immobilization was observed at a concentration of 100 mg/L under open static conditions. In a separate non-GLP study the stability of the test item in Elendt M4 for daphnids was confirmed over a period of 48 h in open vessels and after deep-freezing. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No
- Mortality of control: 0%
- Any observations that might cause a difference between measured and nominal values: The test medium was a clear preparation and stayed unchanged throughout the study. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, the result was within the published ranges of 0.6 - 1.7 mg/L (EC No. 440/2008) and 0.6 - 2.1 mg/L (OECD 202).
- Relevant effect levels: EC50 (48 h) = 0.82 mg/L
- Other: The last reference test dates from 13 - 15 Oct 2016. - Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Reference
VALIDITY CRITERIA
The test fulfilled the validity criteria of the guideline (Table 1).
Table 1: Validity criteria of OECD guideline 202.
Criterion from the guideline |
Study results |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
No immobilization in the control group. |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
The dissolved oxygen concentration at the end of the exposure period was ≥ 3 mg/L. |
Yes |
Description of key information
EC50 (48 h) > 100 mg/L (nominal, OECD 202, D. magna)
Key value for chemical safety assessment
Additional information
There is one study available, in which the short-term toxicity of 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-1,3-thiazol-3-ium-5-yl}ethyl hydrogen phosphate (CAS 10023-48-0) to aquatic invertebrates was assessed according to OECD guideline 202 and GLP.
In a static limit test, Daphnia magna was exposed to a limit concentration of 100 mg/L test item in open test vessels for 48 h. The test item concentration was analytically verified at test start (0 h) and at test end (48 h) by HPLC-UV.
The measured test item concentration was 96.04 mg/L after 0 h and 96.29 mg/L after 48 h, indicating that the substance was stable in Elendt M4 medium for the duration of the test. Since the actual test item concentration was within ± 20% of the nominal concentration, the effect concentration was based on the nominal concentration. After 48 h, no immobilization was observed at the nominal limit concentration of 100 mg/L and the reported EC50 (48 h) is > 100 mg/L.
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