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EC number: 700-169-7 | CAS number: 7646-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 June 2006 to 20 July 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch number: 050804
Purity: >99% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: At the start of the definitive test, four samples (20 mL) were taken from the freshly-prepared control and test media. After 48 hours, the contents of the vessels from each group were pooled and four samples (20 mL) were taken for analysis.
- Sample storage conditions before analysis: stored refrigerated - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance (100 mg) was dissolved in dilution medium (1 l) in a volumetric flask. The contents of the flask were shaken and used directly as the test medium.
- Concentration of vehicle in test medium: The range finding test employed nominal test concentrations of 1, 10 and 100 mg/l. Based on the results of this range finding test, the definitive test employed a single concentration, nominally 100 mg/l.
- Evidence of undissolved material: none, substance highly soluble. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Freshwater (Straus)
- Source: Institute National de Recherche Chimique Appliqué (IRChA), France.
- Age at study initiation (mean and range, SD): <24h
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test: no feed
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): same
- Type and amount of food: unicellular green alga, Pseudokirchneriella subcapitata
- Feeding frequency: Daily except during the initial three days when a slightly lower ration was given.
- Health during acclimation (any mortality observed): healthy - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- The total hardness and alkalinity of the batch of Elendt M4 medium used were 258 and 36 mg/l as CaCO3, respectively.
- Test temperature:
- Continuous monitoring of air temperature in the test area = 19.6 to 22.7°C (see protocol deviations).
- pH:
- 7.66 to 8.08
- Dissolved oxygen:
- 93 to 95%
- Salinity:
- n/a
- Nominal and measured concentrations:
- nominal 100 mg/l and measured 96.4 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 mL
- Volume of solution: 100 mL
- Aeration: no supplementary aeration was employed during the exposure period
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 20 mL per organism
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light: 8 hours dark
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The numbers of mobile, immobile and floating daphnia were counted 24 and 48 hours after the start of the study.
RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 96.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: After 48 hours, one daphnia was observed to be trapped at the surface layer (floating) at 100 mg/L; no immobility had occurred.
- Other biological observations: The measurements of water quality (temperature, pH, dissolved oxygen and total hardness) remained within acceptable limits during the study. The test medium was clear and colourless. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l.
The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l. - Executive summary:
The acute toxicity of HEAA to Daphnia magna was assessed under static exposure conditions. The study was conducted in accordance with OECD Guideline 202, under GLP.
The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l. The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.
Reference
Description of key information
The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l. The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 96.4 mg/L
Additional information
The acute toxicity of HEAA to Daphnia magna was assessed under static exposure conditions. The study was conducted in accordance with OECD Guideline 202, under GLP.
The 48-hour EC50 of HEAA for the immobilisation of Daphnia magna could not be identified but must be >96.4 mg/l. The ‘no-observed effect concentration’ of HEAA with Daphnia magna was 96.4 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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