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EC number: 445-630-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 445-630-0
- EC Name:
- -
- Molecular formula:
- Unspecified
- IUPAC Name:
- 1-(acetyloxy)but-3-en-2-yl acetate; 2-hydroxybut-3-en-1-yl acetate
- Details on test material:
- - Test substance: Vinylglykoldicatet ca. 75% ig
- Batch No.: Kanister 1,2,4 und 5
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Test organism: Danio rerio (formerly Brachydanio rerio)
- Common name: zebra fish
- Supplier: Zierfisch-Center Klöckner, Ludwigshafen
- Body length: 2.6 cm (range 2.3 - 2.9 cm)
- Body weight: 0.14 g (range 0.11 - 0.2 g)
Housing and adaption
- Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system
- Water temperature: Approx. 23 - 25 °C
- Duration of housing: 14 days
- Mortality during the last week prior test start: 0 %
- Medical treatment: none
- Diet: Tetramin fish food daily, ad libitum, additionally live brine shrimp (artemia) on workdays
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- ca. 2.5 mmol/l
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 5.3 - 8.9
- Details on test conditions:
- Test procedure
- Test water: Dechlorinated and charcoal-filtered tap water, aerated.
- Total hardness: approx. 2.5 mmol/l
- Acid capacity: approx. 5.5 mmol/l
- Ratio Ca/Mg ions:approx. 8:1
- pH: generally 7.5 - 8.5
- Volume of water:10 l
- No. of animals per test concentration and control: 10
- Loading rate (g fish/l test water): 1.8
- Test vessels: 10 l glass flasks
- Test temperature: 24 °C
- Adaption to test water and test temperature: 14 days
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: approx. 82 - 280 lux
- Aeration: None
- Exposure period: 96 h
- Test media renewal period: 48 h
- pH during the test: 7.7 - 8.0
- Oxygen content during the test: 5.3 - 8.9 mg/l
- Withdrawal of food: 1 day before and during exposure.
- Nominal test concentrations: 1, 2.2, 5, 10, 22 and 50 mg/l (based on the results of a range-finding test). In addition, a control was tested in parallel.
- Observation time: 1, 4, 24, 48, 72 and 96 h.
- Endpoints investigated: mortality and symptoms of intoxication.
- Preparation of the test item: The test concentrations were freshly prepared separately for each test vessel. The test substance was weighed in to a beaker. The defined volume of test water was filled in to the test vessel. An aliquot from the test water in the test vessel was taken and mixed with the test substance using a glass rod and the mixture was poured into the test vessel and the whole test batch was homogenized again with a glass rod.
- Analytical dose verification: Samples from each test concentration and the control at the start and the end of the two intervals were taken and analysed using HPLC-method.
- Statistical evaluation of data: The LC50 was determined using probit analysis. The NOEC, the LC0, the LOEC and the LC100 were determined directly from the raw data. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10.9 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 30.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- RS-Freetext:
During the 96 h exposure to vinylglykoldicatetat 75% ig, mortalities were as follows:
Nominal test Cumulative mortalitiy (%) after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96
(mg/l)
Control 0 / 0 / 0 / 0 / 0 / 0
1 0 / 0 / 0 / 0 / 0 / 0
2.2 0 / 0 / 0 / 0 / 0 / 0
5 0 / 0 / 0 / 0 / 0 / 0
10 0 / 0 / 0 / 0 / 0 / 0
22 0 / 0 / 0 / 3 / 5 / 7
50 0 / 0 / 10 / 10 / 10 / 10
Symptoms of intoxication:
Nominal test Symptoms of intoxication after x hours
concentration 1 / 4 /24 / 48 / 72 / 96
(mg/l)
Control - / - / - / - / - / -
1 - / - / - / - / - / -
2.2 - / - / - / - / - / -
5 - / - / - / - / - / -
10 - / - / - / - / - / -
22 - / - / - / - / - / -
50 - / - / + / + / + / +
Definitions of Symptoms:
-: No symptoms
+: All fish dead
Analytical results:
Nominal test concentration / mean measured concentration / recovery (% of nominal)
(mg/l)
Control n.d. -
1 0.6 60.0
2.2 1.3 59.1
5 2.9 58.0
10 6.0 60.0
22 12.5 56.8
50 30.9 61.8
n.d. - not detectable
The analytical results were out of +/- 80 % of the nominal values. Therefore, all biological results were refered to mean measured test concentrations of vinylglykoldiacetat 75% ig.
Behavior of the test item during the test:
No remarkable observations.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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