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EC number: 242-809-3 | CAS number: 19090-60-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Adipic acid, ammonium salt
- EC Number:
- 242-809-3
- EC Name:
- Adipic acid, ammonium salt
- Cas Number:
- 19090-60-9
- Molecular formula:
- C6H10O4.xH3N
- IUPAC Name:
- ammonium 5-carboxypentanoate
- Test material form:
- solid: crystalline
1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For determination of the test item concentrations, four replicate samples (3 mL per replicate) were taken from the test solution (100 mg/L) and from the control at the start and at the end of the test.
Test solutions
- Vehicle:
- yes
- Remarks:
- water
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test System (Test Animals)
Species and strain: Daphnia magna (Straus)
Origin: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection)
2100 Gödöllő, Kotlán S. u. 3. Hungary
Breeding: The Daphnia are bred in the Laboratory of TOXI-COOP ZRT.
Number of animals: Twenty animals in both groups (concentration and untreated control), divided into 4 concurrent batches (5 animals per batch).
Age of animals: Less than 24 h old at the beginning of the test.
Sex: Female
Animal health: Apparently healthy animals were used in this test with a known history
Acclimatization: Test animals were bred under similar (or the same) conditions as that used during the exposure period (holding water, temperature, background colour etc.), therefore additional acclimatisation before the test was not necessary. Brood daphnids were maintained in dilution water at the test temperature for at least 48 hours prior to the start of the test.
Food and Feeding: The Daphnia were fed with concentrated algal suspension of Raphidocelis subcapitata during the holding. Test animals were not fed during the exposure.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Hardness: The reconstituted water (ISO medium) had an approximate theoretical total hardness of 249 mg/L (as CaCO3).
- Test temperature:
- Temperature: The test temperature was in the range of 20.8 – 21.1 °C measured in the test vessels during the test. The additionally measured ambient temperature within the climate chamber was between 20.4 – 21.5 °C.
- pH:
- pH: The pH of the test solution was not adjusted and not varied by more than 1.5 units in any one test group. The pH was in the range of 7.82 – 8.28 during the test.
Remark: The data of pH, dissolved oxygen concentration and temperature measured during the test are detailed in Appendix I; Tables 3-5. - Dissolved oxygen:
- Oxygen Concentration: The dissolved oxygen concentration was in the range of 7.26 – 8.06 mg/L during the test.
- Salinity:
- na
- Conductivity:
- na ( reconstituted water ISO medium)
- Details on test conditions:
- Table 3: Test Item concentration measured at the start
Nominal concentration
mg/L Mean of the measured concentrations
mg/L Measured concentration in % of the nominal
100 98.1 (RSD: 1 %) 98
Control below quantitation limit -
Table 4: Test Item concentration measured at the end
Nominal concentration
mg/L Mean of the measured concentrations
mg/L Measured concentration in % of the nominal
100 106.4 (RSD: 1 %) 106
Control not detected - - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item Ammonium Adipate had no toxic effect at 100 mg/L concentration (i.e. limit test concentration) on Daphnia magna; the EC50 and the LOEC are higher than 100 mg/L.
All validity criteria were met. The results are based on nominal test item concentrations.
Summary of the Biological Endpoints (same a Table 1)
Endpoint Concentration [mg/L]
48 h-EC10 > 100
48 h-EC20 > 100
48 h-EC50 > 100
48 h-NOEC 100
48 h-LOEC > 100
- Executive summary:
Test item:
Ammonium Adipate(Lot No.:N118031457)
Test species:
Daphniamagna(Straus)
Dilution water:
ISO medium, prepared inthe laboratory of TOXI-COOP ZRT.
Concentrations:
Limit test; using a single concentration of 100 mg/L; concurrent control ran.
Test design:
Twenty animals, divided into four groups (glass beaker; volume app. 50 mL) of five animals each were exposed to the test concentration or run as control for 48 hours in a static test.
The immobility or mortality of theDaphniais determined by visual observation after 24 and 48 hours of exposure.
Analytics:
Concentration of the test item were determined usingHPLC-UV methodatthe start and at the end of the test.
Endpoints:
EC10, EC20, EC50, NOEC and LOEC for 48h test period.
Statistics:
Statistical analysis was not necessary; endpoints were determined directly from the raw data.
Validity:
All validity criteria were met and therefore the study can be considered as valid (see section 8.1)
Results:
There was no immobilisation observed in twenty daphnids exposed to each test item treated and control group. Further no abnormal behaviour or appearance of test animals was noted.
Biological results are based on the nominal test item concentrations.
The biological endpoints are summarised below in Table 1.
Table 1:Summary of the Biological Endpoints
Endpoint
Concentration
[mg/L]48 h-EC10
> 100
48 h-EC20
> 100
48 h-EC50
> 100
48 h-NOEC
100
48 h-LOEC
> 100
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