4-amino-N-(4-aminophenyl)benzenesulphonamide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

Data is from study report.

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide
- Molecular formula : C12H13N3O2S
- Molecular weight : 263.32 g/mol
- Smiles notation : O=S(=O)(c1ccc(cc1)Nc1ccc(cc1)N)N
- InChl : 1S/C12H13N3O2S/c13-9-1-3-10(4-2-9)15-11-5-7-12(8-6-11)18(14,16)17/h1-8,15H,13H2,(H2,14,16,17)
- Substance type : Organic
- Physical state : Solid

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively.
- Sampling method: The sample was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg / l and 250 mg / l, a slight precipitate was seen at the end of the test.

Vehicle:

not specified

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Species: Pseudomonas putida
- Strain: DSM 50026
- Growth period: 7 ± 1 h

Test type:

not specified

Water media type:

not specified

Remarks on exposure duration:

Total duration of study was not mentioned.

Test temperature:

297K ± 1K

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer
- Material, size, headspace, fill volume: Nominal volume and net volume of the test vessel was 300 and 100 ml, respectively.
- No. of vessels per concentration (replicates): 1
- Nutrients provided for bacteria:
0.5 ml nutrient concentration I (20 subject)
0.5 ml nutrient concentration II (20 subject)

TEST MEDIUM / WATER PARAMETERS: AK-Medium DIN 38412 Part 8 draft was used as a test medium for the study.

TEST CONCENTRATIONS
- Test concentrations: Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively.

Reference substance (positive control):

not specified

Key result

Dose descriptor:

EC10

Effect conc.:

62.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Other details not known

Key result

Dose descriptor:

EC50

Effect conc.:

242.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Other details not known

Experimental results

Conc. In mg/l	E 436 nm			Percentage of the control
	Inoculated	Uninoculated	Difference
Control inoculated		0.354
0.98 1.95 3.91 7.91 15.265 31.25 62.5 125 250 500	0.39 0.354 0.408 0.408 0.412 0.375 0.334 0.286 0.226 0.19	0.008 0.005 0.005 0.002 0.004 0.006 0.016 0.028 0.054 0.107	0.382 0.349 0.403 0.0406 0.408 0.369 0.318 0.258 0.172 0.083	107.99 98.73 113.93 114.71 115.28 104.38 89.89 72.84 48.51 23.48

EC 10 = 62.3 mg/l

EC 50 = 242.4 mg/l

EC 90 = ---

 

Conc.	Individual values	Sample inoculated	Net values	Standard deviation	Variance in %
0.98	0.398 0.39 0.385 0.386	0.008	0.382	0.006	1.52
1.95	0.284 0.415 0.348 0.369	0.005	0.349	0.054	15.37
3.91	0.444 0.356 0.397 0.434	0.005	0.403	0.04	9.81
7.91	0.313 0.4 0.473 0.444	0.002	0.406	0.07	17.12
15.625	0.372 0.456 0.388 0.43	0.04	0.408	0.038	9.34
31.25	0.337 0.457 0.339 0.367	0.006	0.369	0.056	15.08
62.5	0.3 0.315 0.355 0.365	0.018	0.318	0.031	9.35
125	0.226 0.34 0.262 0.314	0.028	0.258	0.851	17.95
250	0.207 0.234 0.236 0.225	0.054	0.172	0.013	5.87
500	0.186 0.202 0.182 0.19	0.107	0.083	0.009	4.55

 

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

Executive summary:

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K. The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

Description of key information

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K (Sustainability Support Services (Europe) AB, Report no. DUU/WL-A 520, 1987). The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.

Key value for chemical safety assessment

EC50 for microorganisms:

242.4 mg/L

EC10 or NOEC for microorganisms:

62.3 mg/L

Additional information

Toxicity to micro-organism study was conducted using Pseudomonas putida strain DSM 50026 at a temperature of 297K ± 1K (Sustainability Support Services (Europe) AB, Report no. DUU/WL-A 520, 1987). The sample 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide (CAS no. 16803 -97 -7) was dissolved for 10 minutes at about 353 K under stirring at 150 rpm. At the concentrations of 500 mg /l and 250 mg /l, a slight precipitate was seen at the end of the test. Test chemical concentration used for the study were 0.98, 1.95, 3.91, 7.91, 15.265, 31.25, 62.5, 125, 250 and 500 mg/l, respectively. Pseudomonas putida strain DSM 50026 was used as a test organism which was grown in an AK-Medium DIN 38412 for a period of 7 ± 1 h. Erlenmeyer was used as a test vessel with a nominal volume and net volume of 300 and 100 ml, respectively. Based on the effect of the test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on Pseudomonas putida, the EC10 and EC50 value was determined to be 62.3 and 242.4 mg/l, respectively.