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EC number: 239-799-8 | CAS number: 15707-23-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2002-09-04 to 2002-10-08
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, D-31137 Hildesheim, Germany
- Pretreatment: Filtered through folded filter. The second effluent was used for initiation of inocilation.
- Initial cell/biomass concentration: 10^4 - 10^6 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3.5 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral nutrient solution according to OECD 301 D
- Test temperature: 20 — 21 °C
- pH: 7.49 - 7.56
- Aeration of dilution water: till 20 h before the start of the test
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: BOD bottles with glass stoppers (volume 300 mL)
- Number of culture flasks/concentration:
10 bottles containing test item and inoculum (test suspension);
10 bottles containing only inoculum (inoculum control);
10 bottles containing reference item and inoculum (functional control);
10 bottles containing test item and reference item and inoculum (toxicity control);
CONTROL AND BLANK SYSTEM
- Inoculum blank: Nutrient solution and inoculum
- Toxicity control: 1.75 mg/L test item + 5.0 mg/L reference item + nutrient solution and inoculum - Reference substance:
- acetic acid, sodium salt
- Preliminary study:
- The ThOD of 2.095 mg O2 / mg test item was used for caiculation of BOD and biodegradation in %.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7
- Sampling time:
- 28 d
- Details on results:
- The test item did not reach the 10 % level (begin of degradation). The pass level of a biodegradation of > 60 % was not reached neither in the 10-d window nor after 28 days.
- Results with reference substance:
- The pass level of a biodegradation > 60 % was reached after 4 days. The validity criterion that the degradation should be > 60 % after 14 d was fulfilled. After 14 days the biodegradation was determined to 97 %.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The ready biodegradability of the test substance was investigated according to the OECD guideline 301 D: Ready Biodegradability: Closed bottle test (1992). Under the test conditions, the test substance was found to be not readily biodegradable over the test period of 28 days. Furthermore, the test substance showed no inhibitory effect on the microorganisms. At the end of the test, the test substance did not reach the 10 % level of degradation.
- Executive summary:
The ready biodegradability of the test substance was investigated in accordance with the OECD guideline 301 D: Ready Biodegradability: Closed bottle test (1992). The biodegradation of the test substance was followed by exposing it to microorganisms from the secondary effluent of a domestic activated sludge over 28 days. The method assesses the ready biodegradability of a test substance under aerobic conditions at 20 ± 1°C in the dark. During this period the biodegradation was determined on the basis of the reduction of dissolved oxygen. The degree of biodegradation was calculated by the amount of oxygen taken up by the test substance (corrected for that in the blank inoculum control) as a percentage of ThOD or COD. As a reference compound (functional control control), 10 mg/L of sodium acetate was tested simultaneously under the same conditions. In the toxicity control, the test substance showed no inhibitory effect on the microorganisms at the test concentration of 1.75 mg/L. In the functional controls, the reference compound sodium acetate was readily biodegraded by an average of 99 % after 28 days of exposure. At the end of the test (28 days), the test substance was biodegraded by an average of 7 %. Therefore, for the test substance no biodegradation was observed under the test conditions.
Reference
In the case of the functional control, the adaptation phase transformed to a degradation phase after 1 day (degradation > 10 %). The pass level > 60 % was reached after 6 days. After 21 days the biodegradation came to 100 % and the plateau was reached.
The biodegradation rate of the toxicity control came to 48 % after 14 days and to a maximum of 54 % after 21 days.
The test item did not reach the 10 % level (begin of degradation). The pass level of a biodegradation of > 60 % was not reached neither in the 10 - d - window nor after 28 days.
Table 1. Oxygen Determination of the Test Item and the Toxicity Control
Study day [d] |
Test item 3.5 mg/L ThOD 2.095 mg O2/mg |
Toxicity control 1.75 mg/L test item + 5 mg/L reference item ThOD 1.12 mg O2/mg |
||||||||||
O2[mg/L] |
BOD |
degr. |
|
|||||||||
P1 |
P2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
O2[mg/L] |
BOD |
degr. |
||||
T1 |
T2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
|||||||
0 7 14 21 28 |
10.68 9.01 8.77 8.44 8.68 |
11.11 9.09 8.61 8.39 8.33 |
10.90 8.05 8.69 7.42 8.51 |
- 0.47 0.45 0.68 0.50 |
- 0.13 0.13 0.19 0.14 |
- 6 6 9 7 |
11.10 5.70 5.38 5.10 5.94 |
10.95 5.66 5.84 5.28 5.36 |
11.03 5.68 5.61 5.19 5.65 |
- 3.97 3.66 4.04 3.49 |
- 0.59 0.54 0.60 0.52 |
- 53 48 54 46 |
Table 2. Oxygen Determination of the Inoculum Control and Functional Control
Study day [d] |
Inocolum control O2[mg/L] |
Functional control 10 mg/L ThOD 0.78 mg O2/mg |
||||||||
C1 |
C2 |
mv |
O2-depl. |
O2[mg/L] |
BOD |
Degr. |
||||
R1 |
R2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
|||||
0 7 14 21 28 |
9.76 8.36 8.15 8.10 8.05 |
10.11 8.75 8.20 8.18 8.05 |
9.94 8.56 8.18 8.14 8.05 |
- 1.38 1.76 1.80 1.89 |
11.00 2.15 1.93 1.58 1.57 |
11.19 2.20 1.58 1.24 1.48 |
11.10 2.18 1.76 1.41 1.53 |
- 7.54 7.58 7.89 7.68 |
- 0.75 0.76 0.79 0.77 |
- 96 97 101 99 |
mv = mean value
BOD = Biological oxygen demand
degr. = degradation
O2- depl. = Oxygen depletion
ThOD = Theoretical oxygen demand
Description of key information
The ready biodegradability of the test substance was investigated according to the OECD guideline 301 D: Ready Biodegradability: Closed bottle test (1992). Under the test conditions, the test substance was found to be not readily biodegradable over the test period of 28 days. Furthermore, the test substance showed no inhibitory effect on the microorganisms. At the end of the test, the test substance did not reach the 10 % level of degradation.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test substance was investigated in accordance with the OECD guideline 301 D: Ready Biodegradability: Closed bottle test (1992). The biodegradation of the test substance was followed by exposing it to microorganisms from the secondary effluent of a domestic activated sludge over 28 days. The method assesses the ready biodegradability of a test substance under aerobic conditions at 20 ± 1°C in the dark. During this period the biodegradation was determined on the basis of the reduction of dissolved oxygen. The degree of biodegradation was calculated by the amount of oxygen taken up by the test substance (corrected for that in the blank inoculum control) as a percentage of ThOD or COD. As a reference compound (functional control control), 10 mg/L of sodium acetate was tested simultaneously under the same conditions. In the toxicity control, the test substance showed no inhibitory effect on the microorganisms at the test concentration of 1.75 mg/L. In the functional controls, the reference compound sodium acetate was readily biodegraded by an average of 99 % after 28 days of exposure. At the end of the test (28 days), the test substance was biodegraded by an average of 7 %. Therefore, for the test substance no biodegradation was observed under the test conditions.
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