Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 233-149-7 | CAS number: 10045-86-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, data originate in part from a secondary source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L. Samples were taken at 0 and 48 h
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of test substance was prepared by adding 1.0111g to the test water and fill up to the marker in 100 mL volumetric flask. 2 mL of stock solution was added in the test water and filled up to the marker in 200 mL beaker to prepare test solutions. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation (mean and range, SD): <24 h
- Source: Environmenal toxicity lab., Safety Assessment Center, Korea Testing and Research Institute
- Food type (breeding): unicellular green algae, Chlorella vulgaris, daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 224 mg/L CaCO3
- Test temperature:
- 20.28 ± 0.2 °C
- pH:
- 7.8-8.14
- Dissolved oxygen:
- 7.73-8.18 mg/L
- Nominal and measured concentrations:
- Nominal test concentration: 100 mg/L
The mean concentration was analyzed to be 2.75 mg/L at a nominal concentration of 100 mg/L (2.9% of nominal concentration) at 0 and 48 hours. - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility after 0, 24 and 48 hours
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: control, 0.1, 1, 10, and 100 mg/L.
- Results used to determine the conditions for the definitive study: No immobility was observed at 100 mg/L - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 24 hours and 48 hours exposure, no daphnia was immobilised in 100 mg/L of test solution. No abnormal behavior and intoxication symptoms were observed in control and treatment groups during limit test.
- Reported statistics and error estimates:
- No statistics performed
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- no
- Remarks:
- Conduction of studies under GLP conditions is not a requirement for peer reviewed publications.
- Specific details on test material used for the study:
- 99.9% pure Iron(III) chloride hexahydrate (Wako)
- Analytical monitoring:
- yes
- Remarks:
- ICP-MS
- Details on sampling:
- - Sampling and concentrations: all tested concentrations were sampled at the start and at the end of the experiment
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Iron(III) chloride hexahydrate: Stock solution was prepared by dissolution of Iron(III) chloride hexahydrate in distilled water. Solution was then added to breeding water for the experiments. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: laboratory culture
- Age of parental stock: Female Daphnids (<24 h old), produced by mature females (≥ 2 weeks old), were subcultured every week.
ACCLIMATION
- Acclimation period: Daphnia magna was used for experiments after the acclimation of several generations.
BREEDING:
- Water temperature: 21 ± 1°C
- Light/dark cycle: 16/8 h
- Breeding vessel: In glass flasks using 35 adult daphnids per liter.
- Medium: Tap water in Tsukuba filtered with activated charcoal and renewed every second day.
- Food source: Chlorella vulgaris (diluted to 5 × 10^8 cell/ml)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 21 ± 1°C
- pH:
- 6.5–8.5
- Nominal and measured concentrations:
- Five test concentrations were used up to 100 mg/L.
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: breeding water was used as test medium.
- Intervals of water quality measurement: The pH, dissolved oxygen and temperature were measured at the start and end of tests. The hardness of breeding water was measured in a series of tests.
OTHER TEST CONDITIONS
- Adjustment of pH: yes with 1 M sodium hydroxide
- Photoperiod: 16 h light/8 h dark
- Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- EC50s were estimated by probit analysis.
- Validity criteria fulfilled:
- not specified
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Remarks:
- Summary of available data used for the endpoint assessment of the target substance
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to the analogue justification attached to chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: RA from CAS 7757-93-9
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: iron metal
- Basis for effect:
- mobility
- Remarks on result:
- other: RA from Iron (III) Chloride
Referenceopen allclose all
Table 1. Cumulative immobilization data forDaphnia magna
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Number of daphnids tested |
Cumulative immobilized Daphnia magna (%) |
|
24 hours |
48 hours |
|||
Control |
ND |
30 |
No immobilization |
No immobilization |
100 |
2.9 |
30 |
No immobilization |
No immobilization |
Table 2. Symptoms on intoxication of the test substance toDaphnia magna
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Symptoms of intoxication |
|
24 hours |
48 hours |
||
Control |
ND |
NOR (30) |
NOR (30) |
100 |
2.9 |
NOR (30) |
NOR (30) |
ND : Not detected, ( ) : Number of Daphnia magna, NOR : Normal
Pure Fe (III) was considered to have a low toxicity to D. magna with an EC50 (48 h) of 2.3 mg/L.
Description of key information
No acute toxicity effects towards aquatic invertebrates up to the limit of water solubility.
Key value for chemical safety assessment
Additional information
Iron (III) orthophosphate (CAS 10045-86-0) is a poorly water-soluble substance, as shown by a transformation/dissolution test according to the OECD guideline 29. The short term toxicity of Iron (III) orthophosphate towards aquatic invertebrates was evaluated in a weight of evidence approach with read across data. The short term effects by dissolved Fe3+were assessed using a soluble iron (III) form (FeCl3) while the acute toxicity of PO43-was assessed based on the reference substance Calcium hydrogenorthophosphate (CAS 7757-93-9). An extensive read across justification is provided in IUCLID chapter 13.
The potential toxicity of dissolved iron (III) to aquatic invertebrates was studied by Okamotoa et al. (2014) following the OECD guideline 202. A soluble iron (III) source (i.e. FeCl3) was used for the study in order to study the effects of free Fe3+ions. Daphnia magna was used as the test organism and was exposed in a static regime to 5 test item concentrations up to 100 mg/L (nominal) for 48 h. An analytical monitoring was performed by ICP-MS. Pure Fe (III) was considered to have a low toxicity to D. magna with an EC50 (48 h) of 2.3 mg Fe3+/L, corresponding to an ERVcompound (ecotoxicity reference value) for Iron (III) orthophosphate of 6.34 mg/L. The transformation/dissolution test according to OECD 29 showed that the release of iron from the substance after 7 days was lower than the LOD of 0.1682 µg/L at a loading of 1 mg/L (pH 6). This concentration is far below the conservative effect concentrations found in the study with FeCl3as reference substance. Therefore, no effects from the dissociated iron species released by the substance are expected up to the water solubility of Iron (III) orthophosphate. It has to be emphasized that the effect values with the soluble iron (III) form represent a worst case since iron (III) is under standard environmental conditions not mobile and will rapidly precipitate to poorly water soluble substances.
The acute toxicity of Calcium hydrogenorthophosphate (CAS 7757-93-9) to aquatic invertebrates was investigated in a study following OECD guideline 202 (2013). The study was conducted as a limit test under static conditions. Daphnia magna were exposed to a nominal test substance concentration of 100 mg/L for 48 h. The mean measured substance concentration was 2.75 mg/L. Mortality or symptoms of intoxication were not observed. At test termination an EC50(48 h) of >2.75 mg/L (nominal: EC50(48 h) >100 mg/L) based on the mean measured concentration was determined. Based on the study results Calcium hydrogenorthophosphate (CAS 7757-93-9) is not considered acutely toxic to aquatic invertebrates. Since Calcium hydrogenorthophosphate will dissociate to its ions calcium and phosphate and its solubility is much higher than iron (III) orthophosphate, it is concluded that similarly no effects are expected by the phosphate ions resulting from Iron (III) orthophosphate.
In addition, the publically available Renewal Assessment Report (2013) for use of ferric phosphate in plant protection products also concludes based on experimental data with the active substance that the substance is not acutely toxic to aquatic invertebrates up to a loading rate of 100 mg/L. The study was conducted according to the OECD guidelines 202 and GLP. These data support the above conclusions from the read across data.
In conclusion based on the above described results, no short term effects by Iron (III) orthophosphate towards aquatic invertebrates are expected.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)