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EC number: 219-552-0 | CAS number: 2460-77-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29.09.2017-28.12.2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Yasho Indutries Pvt. Ltd. / 20016011417
- Expiration date of the lot/batch: June 2019
- Purity test date: July 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: forms a suspension in mineral medium at 200 mg/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not specified
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: not specified - Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, adapted
- Details on inoculum:
- - Source of inoculum (e.g. location, sampling depth, contamination history, procedure): Sewage Treat
ment Plant Bengaluru - 560058 India
- Storage conditions: at room temperature, aerated
- Storage length: 5 days
- Preparation of inoculum for exposure: The effluent was allowed to settle for one hour, decanted
and the decanted effluent was used in the test
- Initial cell/biomass concentration: 6.0 x 10^7 CFU/L
- Water filtered: no - Duration of test (contact time):
- 29 d
- Initial conc.:
- >= 10 - <= 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mixture of 24 mL of stock solution A with 1920 mL of Milli-Q water, then 2.4
mL each of stock solutions B, C and D was added and finally the volume was made up to 2400 mL
using Milli-Q water (See tables)
- Test temperature: 21-24 C
- pH: approx. 7.8
- pH adjusted: no
- Aeration of dilution water: with CO2 free air
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Conical flask 5 L - each fitted with an aeration tube reaching nearly to the botto
m of the flask and an outlet
- Number of culture flasks/concentration: 2 / 10-20 mg/L
- Method used to create aerobic conditions: The flasks were aerated with CO2 free air at 46 to 50 mL
/minute
- Test performed in closed vessels due to significant volatility of test substance: no
- Details of trap for CO2 and volatile organics if used:
SAMPLING
- Sampling frequency: Days 2, 4, 7, 9, 12, 15, 17, 21, 25 and 29
- Sampling method: the CO2 was trapped in barium hydroxide and was measured by titration of the
residual hydroxide or as inorganic carbon
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 18.35
- St. dev.:
- 3.274
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 55.76
- St. dev.:
- 2.857
- Sampling time:
- 17 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 66.18
- St. dev.:
- 1.068
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 104.44
- St. dev.:
- 5.176
- Sampling time:
- 29 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- Based on the results, it was concluded that the test item is bidoegradable as 104.44% mean degradation was achieved at the end of the test. Since, the test item degradation reached less than 60% pass level in 10 day window period after reaching 10& of ThCO2 production, the test item is not considered to be readily biodegradable.
- Executive summary:
The ready biodegradability of 2,5 -di tertiary butyl 1,4 -benzoquinone (Yapox 2255) was tested using the CO2 Evolution Test. The test item was added to two vessels at the concentration of 60 mg/L of mineral medium (equivalent to 15.3 mg of Total Organic Carbon/L). Two control treatments containing only the medium only the inoculum, one reference item treatment and one toxicity control treatment containing the test item and the reference item were also tested. All the treatments were added with equal volume of inoculum which was collected from the secondary effluent treatment plant receiving predominantly domestic sewage.
Treatment mixtures were aerated for 29 days with carbon dioxide (CO2) free air. The CO2 released was trapped in a series of bottles containing barium hydroxide, which were connected to the outlet of each vessel. The residual barium hydroxide was measured on Days 2, 4, 7, 9, 12, 15, 17, 21, 25 and 29 after the initiation of the test.
The mean per cent degradation of test item was 104.44% at the end of the test while, the percent degradation of reference item was 123.02% and the toxicity control was 123.46% at the end of the test. It was observed that the mean degradation of test item reached 18.35% on Day 7 and further reached 55.76% on Day 17; and 66.18% on Day 21. Therefore, the degradation of test itme, 2,5 -di tertiary butyl 1,4 -benzoquinone (YAPOX 2255) reached less than 60% pass level in 10 day window period after reaching 10% of ThCO2 production and within the 28 -d period of the test.
The test fulfilled the validity criteria.
Based on the results, it was concluded that the test item is biodegradable as 104.44% mean degradation was achieved at the end of test. Since, the test item degradation reached less than 60% pass level in 10 day window period after reaching 10% of ThCO2 production, the test item is not considered to be readily biodegradable.
Reference
Table 1: Carbon dioxide production
Day | CO2 Produced (mg) | Cumulative CO2 produced (mg) Test - Blank Mean |
|||||||||
Test Flasks | Blank | ||||||||||
Flask 1 | Flask 2 | Flask 5 | Flask 6 | Flask 3 | Flask 4 | Mean | Flask 1 | Flask 2 | Flask 5 | Flask 6 | |
Day 2 | 17.49 | 18.26 | 23.54 | 18.81 | 9.13 | 8.91 | 9.02 | 8.47 | 9.24 | 14.52 | 9.79 |
Day 4 | 19.69 | 22.44 | 32.45 | 26.07 | 14.63 | 16.61 | 15.62 | 12.54 | 16.06 | 31.35 | 20.24 |
Day 7 | 25.96 | 30.25 | 40.92 | 31.02 | 12.21 | 10.78 | 11.5 | 27 | 34.81 | 60.77 | 39.76 |
Day 9 | 29.15 | 28.49 | 43.12 | 34.54 | 11.22 | 13.97 | 12.6 | 43.55 | 50.7 | 91.29 | 61.7 |
Day 12 | 34.98 | 36.41 | 37.18 | 34.1 | 14.85 | 11.11 | 12.98 | 65.55 | 74.13 | 115.49 | 82.82 |
Day 15 | 30.03 | 25.96 | 26.84 | 39.82 | 10.89 | 8.47 | 9.68 | 85.9 | 90.41 | 132.65 | 112.96 |
Day 17 | 20.35 | 22.66 | 32.23 | 34.98 | 16.94 | 14.52 | 15.73 | 90.52 | 97.34 | 149.15 | 132.21 |
Day 21 | 32.12 | 27.83 | 26.84 | 25.63 | 10.78 | 14.08 | 12.43 | 110.21 | 112.74 | 163.56 | 145.41 |
Day 25 | 53.35 | 53.02 | 20.9 | 31.79 | 13.75 | 12.98 | 13.37 | 150.19 | 152.39 | 171.09 | 16.83 |
Day 29 | 27.28 | 44.88 | 49.83 | 52.14 | 35.64 | 0 | 17.82 | 159.65 | 179.45 | 203.1 | 198.15 |
Day 29 | 10.12 | 2.64 | 1.43 | 8.47 | 0 | 0 | 0 | 169.77 | 182.09 | 204.53 | 206.62 |
Total CO2 produced in the inoculum blank during the test = 130.75 mg/3L = 43.58 mg/L
Note: Flask 1 and 2: Contain test item (methyl eugenol) and inoculum in mineral media
Flask 3 and 4: Contain inoculum in mineral media
Flask 5: Contain reference item (sodium benzoate) and inoculum in mineral media
Flask 6: Contain test item, reference item and inoculum in mineral media
Table 2: ph of Test Solutions
Flask No. | pH at the end of the test (Day 28) |
Flask 1 | 7.83 |
Flask 2 | 7.8 |
Flask 3 | 7.82 |
Flask 4 | 7.79 |
Flask 5 | 7.82 |
Flask 6 | 7.84 |
Note: Flask 1 and 2: Contain test item (methyl eugenol) and inoculum in mineral media
Flask 3 and 4: Contain inoculum in mineral media
Flask 5: Contain reference item (sodium benzoate) and inoculum in mineral media
Flask 6: Contain test item, reference item and inoculum in mineral media
Table 3: Degradation of test item and refernce item
Day | Test item | Reference item | Test item + Refernce item | ||
Flask 1 | Flask 2 | Mean | |||
Day 2 | 5.03 | 5.49 | 5.26 | 8.73 | 5.85 |
Day 4 | 7.44 | 9.53 | 8.49 | 18.86 | 12.09 |
Day 7 | 16.03 | 20.66 | 18.35 | 36.55 | 23.76 |
Day 9 | 25.85 | 30.1 | 27.98 | 54.91 | 36.87 |
Day 12 | 38.91 | 44.01 | 41.46 | 69.47 | 49.49 |
Day 15 | 50.99 | 53.67 | 52.33 | 79.79 | 67.5 |
Day 17 | 53.74 | 57.78 | 55.76 | 89.71 | 79 |
Day 21 | 65.42 | 66.93 | 66.18 | 98.38 | 86.89 |
Day 25 | 89.16 | 90.46 | 89.81 | 102.91 | 97.9 |
Day 29 | 94.77 | 106.53 | 100.65 | 122.16 | 118.4 |
Day 29 | 100.78 | 108.1 | 104.44 | 123.02 | 123.46 |
% degradation = (mg CO2 produced / (mg TOC added in test x 3.67)) x 100
Note: Flask 1 and 2: Contain test item (methyl eugenol) and inoculum in mineral media
Flask 3 and 4: Contain inoculum in mineral media
Flask 5: Contain reference item (sodium benzoate) and inoculum in mineral media
Flask 6: Contain test item, reference item and inoculum in mineral media
Description of key information
The ready biodegradability of 2,5 -di tertiary butyl 1,4 -benzoquinone (Yapox 2255) was tested using the CO2 Evolution Test. The test item was added to two vessels at the concentration of 60 mg/L of mineral medium (equivalent to 15.3 mg of Total Organic Carbon/L). Two control treatments containing only the medium only the inoculum, one reference item treatment and one toxicity control treatment containing the test item and the reference item were also tested. All the treatments were added with equal volume of inoculum which was collected from the secondary effluent treatment plant receiving predominantly domestic sewage.
Treatment mixtures were aerated for 29 days with carbon dioxide (CO2) free air. The CO2 released was trapped in a series of bottles containing barium hydroxide, which were connected to the outlet of each vessel. The residual barium hydroxide was measured on Days 2, 4, 7, 9, 12, 15, 17, 21, 25 and 29 after the initiation of the test.
The mean per cent degradation of test item was 104.44% at the end of the test while, the percent degradation of reference item was 123.02% and the toxicity control was 123.46% at the end of the test. It was observed that the mean degradation of test item reached 18.35% on Day 7 and further reached 55.76% on Day 17; and 66.18% on Day 21. Therefore, the degradation of test itme, 2,5 -di tertiary butyl 1,4 -benzoquinone (YAPOX 2255) reached less than 60% pass level in 10 day window period after reaching 10% of ThCO2 production and within the 28 -d period of the test.
The test fulfilled the validity criteria.
Based on the results, it was concluded that the test item is biodegradable as 104.44% mean degradation was achieved at the end of test. Since, the test item degradation reached less than 60% pass level in 10 day window period after reaching 10% of ThCO2 production, the test item is not considered to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
- Type of water:
- freshwater
Additional information
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