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EC number: 208-253-0 | CAS number: 518-47-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- Bioaccumulation: aquatic/sediment.
- Author:
- National institute of technology & evaluation
- Year:
- 2 016
- Bibliographic source:
- Japan chemicals collborative knowledge database (J-check)
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Bioaccumulation: aquatic/sediment
- Qualifier:
- according to guideline
- Guideline:
- other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances".
- Principles of method if other than guideline:
- Bioaccumulation test was conducted for 28 days for estimating the bioconcentration factor (BCF) and lipid content of test substance Fluorescein sodium in test organism Cyprinus carpio.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
- EC Number:
- 208-253-0
- EC Name:
- Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
- Cas Number:
- 518-47-8
- Molecular formula:
- C20H12O5.2Na
- IUPAC Name:
- disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
- Reference substance name:
- Fluorescein sodium
- IUPAC Name:
- Fluorescein sodium
- Test material form:
- other: Orange reddish powder
- Details on test material:
- - Name of test material (as cited in study report): Disodium 2-(6-oxido-3-oxo-3H-xanthen-9-yl) benzoate
- Molecular formula (if other than submission substance):
C20H10Na2O5
C20H10O5.2Na
C20H12O5.2Na
- Molecular weight (if other than submission substance): 376.274 g/mol
- Smiles notation (if other than submission substance): c1(c2c(oc3c1ccc(c3)[O-])
cc(=O)cc2)c1c(cccc1)C(=O)[O-].[
Na+].[Na+]
- InChl (if other than submission substance): 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,
(H,23,24);;/q;2*+1/p-2
- Structural formula attached as image file (if other than submission substance): No data available
- Substance type: Organic
- Physical state: Solid
- Analytical purity: No data available
- Impurities (identity and concentrations): No data available
- Composition of test material, percentage of components: No data available
- Isomers composition: No data available
- Purity test date: No data available
- Lot/batch No.: No data available
- Expiration date of the lot/batch: No data available
- Radiochemical purity (if radiolabelling): No data available
- Specific activity (if radiolabelling): No data available
- Locations of the label (if radiolabelling): No data available
- Expiration date of radiochemical substance (if radiolabelling): No data available
- Stability under test conditions: No data available
- Storage condition of test material: No data available
- Other: No data available
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data available - Radiolabelling:
- not specified
Sampling and analysis
- Details on sampling:
- No data available
Test solutions
- Vehicle:
- not specified
- Details on preparation of test solutions, spiked fish food or sediment:
- No data available
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- No data available
Study design
- Test type:
- not specified
- Water / sediment media type:
- not specified
- Total exposure / uptake duration:
- 28 d
Test conditions
- Hardness:
- No data available
- Test temperature:
- No data available
- pH:
- No data available
- Dissolved oxygen:
- No data available
- TOC:
- No data available
- Salinity:
- No data available
- Details on test conditions:
- TEST SYSTEM
- Test vessel: No data available
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume:
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control / vehicle control (replicates): No data available
- Biomass loading rate: No data available
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductance: No data available
- Holding medium different from test medium: No data available
- Intervals of water quality measurement: No data available
- Intervals of test medium replacement: No data available
OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: No data available
- Light intensity: No data available
RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: LC50 (96 hr) >200mg/l, Rice fish (Oryzias latipes)
- Results used to determine the conditions for the definitive study: No data available - Nominal and measured concentrations:
- Nominal concentrations-
1st concentration area: 0.46 mg/l
2nd concentration area: 0.046 mg/l - Reference substance (positive control):
- not specified
- Details on estimation of bioconcentration:
- No data available
Results and discussion
Lipid contentopen allclose all
- Lipid content:
- 4.14 %
- Time point:
- end of exposure
- Lipid content:
- 2.94 %
- Time point:
- start of exposure
Bioaccumulation factoropen allclose all
- Type:
- BCF
- Value:
- <= 0.27 L/kg
- Basis:
- other: Test material
- Remarks on result:
- other: Conc.in environment / dose:0.46 mg/l
- Type:
- BCF
- Value:
- <= 0.27 L/kg
- Basis:
- other: test material
- Remarks on result:
- other: Conc.in environment / dose:0.046 mg/l
- Details on kinetic parameters:
- No data available
- Metabolites:
- No data available
- Results with reference substance (positive control):
- No data available
- Details on results:
- No data available
- Reported statistics:
- No data available
Any other information on results incl. tables
Table: Bioaccumulation test results.
|
6d |
9d |
13d |
21d |
28d |
|
1stconcentration area |
Test substance concentration (mg/l) |
0.462 |
0.459 |
0.459 |
0.460 |
0.462 |
Bio-concentration factor (l/kg) |
≤ 0.27 |
≤ 0.27 |
≤ 0.27 |
≤ 0.27 |
≤ 0.27 |
|
≤ 0.27 |
≤ 0.27 |
≤ 0.27 |
≤ 0.27 |
≤ 0.27 |
||
2ndconcentration area |
Test substance concentration (mg/l) |
0.0459 |
0.0457 |
0.0461 |
0.0457 |
0.0460 |
Bio-concentration factor (l/kg) |
≤ 2.7 |
≤ 2.7 |
≤ 2.7 |
≤ 2.7 |
≤ 2.7 |
|
≤ 2.7 |
≤ 2.7 |
≤ 2.7 |
≤ 2.7 |
≤ 2.7 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test substance concentration in environment was 0.46 mg/l, 0.046 mg/l and bioconcentration (BCF) value of test substance was found to be ≤ 0.27 l/kg, respectively. Thus, the test substance have a low bioconcentration potential in test organism Cyprinus carpio during 28 day period.
- Executive summary:
Bioaccumulation test was conducted for 28 days for estimating the bioconcentration factor (BCF) and lipid content of test substance Fluorescein sodium in test organism Cyprinus carpio.
The purity of test substance by HPLC was found to be 92.1% and water solubility value is ≥ 300 g/l at 20ᵒC (Flask method). The recovery ratio is given-
Test water: 100% (The test solution were input into analytical equipment directly.)
Fish: 93.8%
GLP compliance is followed for biodegradation study.Testing laboratory is Chemicals Evaluation and Research Institute, Japan.
Nominal concentrations used in the study are given-
1stconcentration area: 0.46 mg/l
2ndconcentration area: 0.046 mg/l
Range finding study was carried out onRice fish (Oryzias latipes) and the 96 hr LC50 value was found to be >200mg/l.
By analytical methods, thelimit of quantification was found to be-
Test water:
1stconcentration area: 11 µg/l
2ndconcentration area: 1.1 µg/l
Fish: 120 ng/g
The lipid content at the start and end of exposure was found to be 2.94% and 4.14%, respectively.
The test substance concentration in environment during a period of 28 days was 0.46 mg/l, 0.046 mg/l and bioconcentration (BCF) value of test substance was found to be ≤ 0.27 l/kg, respectively. Thus, the test substance have a low potential of bioaccumulation in test organismCyprinus carpio during 28 day period.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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