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EC number: 202-718-1 | CAS number: 98-97-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: refer below principle
- Principles of method if other than guideline:
- Toxicity to micro-organisms study was conducted on Mycobacterium tuberculosis.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: Pyrazine-2-carboxylic acid
- Molecular formula: C5H4N2O2
- Molecular weight: 124.099 g/mol
- Substance type: organic
- Physical state: solid
- Smiles: c1ncc(C(=O)O)nc1
- InChI: 1S/C5H4N2O2/c8-5(9)4-3-6-1-2-7-4/h1-3H,(H,8,9)
- Radiolabelling: Yes - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 300 µg/ml (300 mg/l) and 100 µg/ml (100 mg/l).
- Vehicle:
- not specified
- Test organisms (species):
- Mycobacter sp.
- Details on inoculum:
- - Preparation of inoculum for exposure: M. tuberculosis culture was prepared in Middlebrook 7H9 medium supplemented with 10% (vol/vol) OADC enrichment (Difco), 0.2% (vol/vol) glycerol, and 0.05% (vol/vol) Tween 80.
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 h
- pH:
- 6 or 6.8
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Test tube
- No. of organisms per vessel: 105 CFU/ml
OTHER TEST CONDITIONS
- Adjustment of pH: pH of the medium was adjusted to 6 or 6.8 with an aqueous solution of 4.25% phosphoric acid or 5% dibasic potassium phosphate.
- Test concentrations: 300 mg/l and 100 mg/l. - Key result
- Duration:
- 72 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: at pH 6.8
- Duration:
- 72 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: at pH 6
- Validity criteria fulfilled:
- not specified
- Conclusions:
- From experimental result the minimum inhibition concentration (MIC) value was observed to be 300 mg/l at pH 6.8 and 100 mg/l at pH 6 at 72 hrs exposure period.
- Executive summary:
Toxicity study of micro-organism to the test substance was conducted using Mycobacterium tuberculosis. When test bacteria was exposed to the test substance pyrazinoic acid, decreased in growth of the bacteria was observed. From experimental result the minimum inhibition concentration (MIC) value was observed to be 300 mg/l at pH 6.8 and 100 mg/l at pH 6 at 72 hrs exposure period.
Reference
Description of key information
Toxicity study of micro-organism to the test substance was conducted usingMycobacterium tuberculosis.When test bacteria was exposed to the test substance pyrazinoic acid, decreased in growth of the bacteria was observed.From experimental result the minimum inhibition concentration (MIC) value was observed to be 300 mg/l at pH 6.8 and 100 mg/l at pH 6 at 72 hrs exposure period.
Key value for chemical safety assessment
Additional information
Five experimental studies from peer reviewed journal for toxicity to micro organisms endpoint of test chemical Pyrazine-2-carboxylic acid (Cas no. 98-97-5) were summarised as follows:
First data from journal Antimicrobial Agents and Chemotherapy, Vol. 51, No. 2, Pg. no. 752–754, Feb. 2007 suggests toxicity study of micro-organism to the test substance was conducted using Mycobacterium tuberculosis.When test bacteria was exposed to the test substance pyrazinoic acid, decreased in growth of the bacteria was observed. From experimental result the minimum inhibition concentration (MIC) value was observed to be 300 mg/l at pH 6.8 and 100 mg/l at pH 6 at 72 hrs exposure period.
Whereas Antimicrobial Agents and Chemotherapy, Vol. 33, No. 8, p. 1252-1254, Aug. 1989 journal indicate the minimum inhibition concentration (MIC) values for different M. tuberculosis strains are observed to be-For M. tuberculosis strains M6733 and W478: 240 µg/ ml (240 mg/l) and For M. tuberculosis strains H37Rv, H6927, H5009, W7804, M6669, T6790, T7505 and 1620: 480 µg/ ml (480 mg/l)
And Journal of Antimicrobial Chemotherapy Vol.53, Pg. no. 192–196, 2004 indicate toxicity study of micro-organism to the test substance was conducted using Mycobacterium tuberculosis BCG strain.When test bacteria was exposed to the test substance pyrazinoic acid with exposure duration of 3 weeks, decreased in cfu counts was observed. From experimental condition the minimum inhibition concentration (MIC) value was observed to be 25 mg/l i.e 4% growth inhibition was occur.
One another journal for target mention the MIC value for different strains of M. tuberculosis of test chemical Pyrazinoic acid (Cas no.98-97-5) are observed as:
For M. tuberculosis strain CDC89 - 1 µg/ml (1 mg/l)
For M. tuberculosis strain CDC72 -≤1 µg/ml (≤1 mg/l)
For M. tuberculosis strain H6777 - 4 µg/ml (4 mg/l)
For M. tuberculosis strain M142, T2614, CA516 and W11053 - 8 µg/ml (8 mg/l)
For M. tuberculosis strain T2603, ATCC 25618, M4812, DHMH 5207 and DHMH 4319 – 16 µg/ml (16 mg/l)
For M. tuberculosis strain DOL, ATCC 27294, M4597, ATCC 35801, F7091, H6128, W6126, DHMH 444, W4693, T5721, ATCC 35828, M4809, VA 205, DHMH 6577, BDDIS 20 and CDC-BP-98 – 32 µg/ml (32 mg/l)
For M. tuberculosis strain H3652 -≤8 µg/ml (≤8 mg/l)
For M. tuberculosis strain T494 – 128 µg/ml (128 mg/l)
And lastly Saudi Pharmaceutical Journal Vol. 22, Pg. no. 376–380, 2014 indicate briefly as toxicity study of micro-organism to the test substance was conducted using Mycobacterium tuberculosis H37Rv (ATCC 27294).When test bacteria was exposed to the test substance pyrazinoic acid with exposure duration of 6 days, decreased in growth of the bacteria was found. The MIC value was found to be 15620000 mg/l.
Thus all minimum inhibition concentration (MIC) values from all studies give the conclusion that test substance Pyrazine-2-carboxylic acid (Cas no. 98-97-5) may have no concern for toxicity to micro organism.
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