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Diss Factsheets
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EC number: 231-679-3 | CAS number: 7681-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary literature
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Toxicity study was conducted to evaluate the effect of test chemical on the cell proliferation of freshwater green algae Scenedesmus quadricauda. Test chemical exposed for 8 days with the green algae.
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was dissolved in bi-distilled water, the stock solution neutralized. Two parallel concentration were used in the series which by gradual dilution (factor: 0.5). Control were also used in the study. - Test organisms (species):
- other: Scenedesmus quadricauda
- Details on test organisms:
- Details on test organisms
TEST ORGANISM
- Common name: Green algae
- Method of cultivation: Algae from permanent cultivation were obtain. New breeding of stock cultures in the 10-day cycle added. Inoculation of the test cultures from 10 days old precultures and same culture conditions for parent, preliminary and test organism were maintain throught the study. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 8 d
- Test temperature:
- 27 degrees C
- pH:
- 7
- Nominal and measured concentrations:
- Nominal concentration
- Details on test conditions:
- Details on test conditions
TEST SYSTEM
- Test vessel: Tubes
- Photoperiod: Continuous light
- Humidity: 50% - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 8 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2 370 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: Other details not known
- Details on results:
- Details on result:
Very low effect were observed on the growth and cell proliferations of green algae after the exposure of test chemical for 8 hrs. - Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the effect observed on the cell proliferation rate of green algae Scenedesmus quadricauda after 8 days of exposure, the LOEC was observed at 2370 mg/l.
- Executive summary:
Toxicity study was conducted to evaluate the effect of test chemical on the cell proliferation of freshwater green algae Scenedesmus quadricauda. Test chemical exposed for 8 days with the green algae. Test performed under the static system by using 10 days old culture. Test substance was dissolved in bi-distilled water, the stock solution neutralized. Two parallel concentration were used in the series which by gradual dilution (factor: 0.5). Control were also used in the study. Algae from permanent cultivation were obtain. New breeding of stock cultures in the 10-day cycle added. Inoculation of the test cultures from 10 days old precultures and same culture conditions for parent, preliminary and test organism were maintain throughout the study. From each inoculated batch, three 10 ml culture tubes were used and one tube per control. Shake the test cultures once a day. Cell density determination at the end of the test photometric at 578 nm. Very low effect were observed on the growth and cell proliferations of green algae after the exposure of test chemical for 8 hrs. Based on the effect observed on the cell proliferation rate of green algae Scenedesmus quadricauda after 8 days of exposure, the LOEC was observed at 2370 mg/l. based on the LOEC, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Reference
Description of key information
Toxicity study was conducted to evaluate the effect of test chemical on the cell proliferation of freshwater green algae Scenedesmus quadricauda. Test chemical exposed for 8 days with the green algae. Test performed under the static system by using 10 days old culture. Test substance was dissolved in bi-distilled water, the stock solution neutralized. Two parallel concentration were used in the series which by gradual dilution (factor: 0.5). Control were also used in the study. Algae from permanent cultivation were obtain. New breeding of stock cultures in the 10-day cycle added. Inoculation of the test cultures from 10 days old precultures and same culture conditions for parent, preliminary and test organism were maintain throughout the study. From each inoculated batch, three 10 ml culture tubes were used and one tube per control. Shake the test cultures once a day. Cell density determination at the end of the test photometric at 578 nm. Very low effect were observed on the growth and cell proliferations of green algae after the exposure of test chemical for 8 hrs. Based on the effect observed on the cell proliferation rate of green algae Scenedesmus quadricauda after 8 days of exposure, the LOEC was observed at 2370 mg/l. based on the LOEC, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2 370 mg/L
Additional information
Various short term studies available for the test chemical were reviewed to determine the toxic nature of test chemical on the growth of aquatic algae and cyanobacteria. The studies are as mentioned below:
Toxicity study was conducted to evaluate the effect of test chemical on the cell proliferation of freshwater green algae Scenedesmus quadricauda. Test chemical exposed for 8 days with the green algae. Test performed under the static system by using 10 days old culture. Test substance was dissolved in bi-distilled water, the stock solution neutralized. Two parallel concentration were used in the series which by gradual dilution (factor: 0.5). Control were also used in the study. Algae from permanent cultivation were obtain. New breeding of stock cultures in the 10-day cycle added. Inoculation of the test cultures from 10 days old precultures and same culture conditions for parent, preliminary and test organism were maintain throughout the study. From each inoculated batch, three 10 ml culture tubes were used and one tube per control. Shake the test cultures once a day. Cell density determination at the end of the test photometric at 578 nm. Very low effect were observed on the growth and cell proliferations of green algae after the exposure of test chemical for 8 hrs. Based on the effect observed on the cell proliferation rate of green algae Scenedesmus quadricauda after 8 days of exposure, the LOEC was observed at 2370 mg/l. based on the LOEC, chemical consider to be nontoxic and not classified as per the CLP classification criteria.
First key study was supported by the second study from secondary source. Study was designed to evaluate the effect of test chemical on the growth of algae. The lowest observed effect concentration (LOEC) value was observed at 2370 mg/L in Scenedesmus quadricauda (Green Algae) in a aquatic toxicity study conducted with test chemical on the basis of Population growth rate. Thus chemical consider to be not classified as per the CLP classification criteria.
Similar study was conducted to determine the effect of test chemical on the growth of algae. Test conducted under the static system. The low observed effect concentration (LOEC) value observed is 66 mg/l in Microcystis aeruginosa (Blue-Green Algae) in a aquatic toxicity study conducted with test chemical on the basis of Population growth rate. As low effect were observed on the growth of algae, EC50 was not obtain. Thus on that basis, chemical not consider to be classified as toxic.
Based on the overall data from secondary sources and, it can be concluded that the substance is considered to be not toxic to aquatic environment (algae and cyanobacteria) and cannot be classified as toxic as per the criteria mentioned in CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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