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EC number: 252-328-0 | CAS number: 35037-73-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
- Principles of method if other than guideline:
- The micronucleus test was employed to investigate 4-trifluoromethoxyphenyl isocyanate in male and female mice for a possible clastogenic effect on the chromosomes of bone-marrow erythroblasts. The known clastogen and cytostatic agent, Trenimon, served as positive control. The treated animals received 2 oral application within 24 hoursof the test substance, the positive control was applied intraperitoneal. The femoral marrow of groups treated with 4-trifluoromethoxyphenyl isocyanate was prepared 6 hours after administration.
- GLP compliance:
- no
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- p-(trifluoromethoxy)phenyl isocyanate
- EC Number:
- 252-328-0
- EC Name:
- p-(trifluoromethoxy)phenyl isocyanate
- Cas Number:
- 35037-73-1
- Molecular formula:
- C8H4F3NO2
- IUPAC Name:
- 1-isocyanato-4-(trifluoromethoxy)benzene
- Details on test material:
- content: 99.78%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: Bor: NMRI (SPF Han)
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Duration of treatment / exposure:
- 2 oral treatments within 24 hours
- Frequency of treatment:
- 2 oral treatments within 24 hours
- Post exposure period:
- Animals were sacrificed 6 after the second application
Doses / concentrationsopen allclose all
- Dose / conc.:
- 100 mg/kg bw/day (nominal)
- Remarks:
- 2 x 100 mg/kg bw
- Dose / conc.:
- 50 mg/kg bw/day (nominal)
- Remarks:
- 2 x 50 mg/kg bw
- No. of animals per sex per dose:
- 5 male and 5 female mice/group
- Control animals:
- yes, concurrent vehicle
Examinations
- Tissues and cell types examined:
- femoral marrow
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- in the 2 x 50 mg/kg bw no signs of toxicity; in the 2 x 100 mg/kg bw group 11 of 20 animals died
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
No signs for a mutagenic effect of 4-trifluoromethoxyphenyl isocyanate in doses up to 2 x 100 mg/kg bw was evident. No relevant, treatment related alteration of the ratio polychromatic to normochromatic erythrocytes was found. the positive control (Tremonin) had a clear mutagenic effect.
Applicant's summary and conclusion
- Conclusions:
- No signs for a mutagenic effect of 4-trifluoromethoxyphenyl isocyanate in doses up to 2 x 100 mg/kg bw was evident. No relevant, treatment related alteration of the ratio polychromatic to normochromatic erythrocytes was found. the positive control (Tremonin) had a clear mutagenic effect. Under the conditions of the test 4-trifluoromethoxyphenyl isocyanate was negative in the in-vivo micronucleus test.
- Executive summary:
The micronucleus test was employed to investigate 4-trifluoromethoxyphenyl isocyanate in male and female mice for a possible clastogenic effect on the chromosomes of bone-marrow erythroblasts. The known clastogen and cytostatic agent, Trenimon, served as positive control. The treated animals received 2 oral application of the test substance within 24 hours, the positive control was applied intraperitoneal. The femoral marrow of groups treated with 4-trifluoromethoxyphenyl isocyanate was prepared 6 hours after administration.
No signs for a mutagenic effect of 4-trifluoromethoxyphenyl isocyanate in doses up to 2 x 100 mg/kg bw was evident. No relevant, treatment related alteration of the ratio polychromatic to normochromatic erythrocytes was found. the positive control (Tremonin) had a clear mutagenic effect. Under the conditions of the test 4-trifluoromethoxyphenyl isocyanate was negative in the in-vivo micronucleus test.
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