p-(trifluoromethoxy)phenyl isocyanate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

4-Trifluoromethoxyphenyl isocyanate was applied semiocclusive 50 , 100, 150, 250, 500, 1000 or 1500 µl/kg bw (male rats) and 50, 100, 250, 500, 1500, or 2500 µl/kg bw (female rats) to five or ten male or female Wister rats for 24 hours. The animals were observed for mortality and clinical signs through day 14. A gross pathological examination was performed on animals which died intercurrent or were killed after termination of the study.

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

p-(trifluoromethoxy)phenyl isocyanate

EC Number:

252-328-0

EC Name:

p-(trifluoromethoxy)phenyl isocyanate

Cas Number:

35037-73-1

Molecular formula:

C8H4F3NO2

IUPAC Name:

1-isocyanato-4-(trifluoromethoxy)benzene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: undiluted or with cellulose powder

Duration of exposure:

24 hours

Doses:

male rats: 50 , 100, 150, 250, 500, 1000 or 1500 µl/kg bw = 67, 134, 201, 335, 670, 1340, 2010 mg/kg bw
female rats: 50, 100, 250, 500, 1500, or 2500 µl/kg bw = 67, 134, 335, 670, 20103350 mg/kg bw

No. of animals per sex per dose:

male rats: 50 µl/kg bw = 5 animals; 100, 150, 250, 500, 1000 or 1500 µl/kg bw = 10 animals
female rats: 50, 2500 µl/kg bw = 5 animals; 100, 250, 500, 1500 µl/kg bw = 10 animals

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 268 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 670 mg/kg bw

Based on:

test mat.

Other findings:

Signs of intoxiciation: increased motility, afterwards decreased motility, aggresivity, laboured breathing, weekness of hind limbs, partly strong weight loss.

Start of the symptoms: 5 to 20 minutes after application. Duration of the symptoms: 2 to 6 days. The surviving animals showed afterwards apathy up to 14 days

Necropsy findings on animals died intercurrent: , liver pale and mottled, kidneys pale, renal plevis partly reddened, in the urinary bladder partly red urine, intestine with dark, mucous content.

Necropsy findings on animals killed after post-observation period: on animals with weekness of hind limbs the renal pelvis was destroyed, bloody urine, other internal organs macroscopically without findings

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Remarks:

Based on EU GHS criteria.

Conclusions:

Signs of intoxication were apathy, decreased motolity, cyanosis, laboured breathing and cramps. The LD50 for female rats was ca. 670 mg/kg bw and the LD50 for male rats was 238 mg/kg bw.

Executive summary:

Acute toxicity dermal test was done similarly to OECD guideline 402. 4-Trifluoromethoxyphenyl isocyanate was applied semiocclusive 50 , 100, 150, 250, 500, 1000 or 1500 µL/kg bw (male rats) and 50, 100, 250, 500, 1500, or 2500 µL/kg bw (female rats) to five or ten male or female Wister rats for 24 hours. The animals were observed for mortality and clinical signs through day 14. A gross pathological examination was performed on animals which died intercurrent or were killed after termination of the study.

Signs of intoxication were apathy, decreased motolity, cyanosis, laboured breathing and cramps. The LD50 for female rats was ca. 670 mg/kg bw and the LD50 for male rats was 238 mg/kg bw.