Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP compliance statement and Quality assurance unit statement available in the study report
Test material
- Reference substance name:
- Fermentation product of sucrose and Theaceae Camellia Camellia sinensis leaves with a variable symbiosis of bacteria and yeast, of including bacteria of Acetoacteraceæ Acetobacter and Gluconobacter genus, and yeasts of Saccharomycetaceae family
- Molecular formula:
- Not applicable
- IUPAC Name:
- Fermentation product of sucrose and Theaceae Camellia Camellia sinensis leaves with a variable symbiosis of bacteria and yeast, of including bacteria of Acetoacteraceæ Acetobacter and Gluconobacter genus, and yeasts of Saccharomycetaceae family
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- other: Deionized water (as a base of M4 medium)
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Observation times: 24 and 48 hours
Test conditions
- Test temperature:
- 19.6 – 20.3 °C
- pH:
- pH 7.0 (0h) and pH 7.4 (48h)
- Dissolved oxygen:
- 8.7 mg 02/L (0h) and 7.8 mg 02/L (48h)
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5)
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- - Immobilisation in the controls may not exceed 10 % (Immobilisation in the control: 0 %) - The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L (Lowest concentration of dissolved oxygen: 7.8 mg/L)
- Conclusions:
- According to OECD 202 test, Daphnia 48h-EC50 for Kombucha ("Fermentation product of sucrose and Camellia sinensis (Theaceae) leaves with a variable symbiosis of bacteria and yeast, including various Acetoacteraceæ bacteria and Saccharomycetaceae yeasts) is > 10 mg/l.
- Executive summary:
One valid experiment was performed.
20 Daphnia were exposed to the test item for the treatment for 48 hours in a static test system.
The tested concentration showed no toxicity. None of the animals were immobilised in the blank control and the treatment.
At the start and at the end of the test, the content of TOC in the blank control and in the test solution was measured using a carbon analyser. The TOC concentrations in the test solution were 225.5 mg/l at the beginning and 166.5 mg/L at the end of the test. The measured TOC concentrations prove that the substance was present in the test solution. The lower measured concentrations at the end of the test were most likely caused by adsorption of the test item to the test organism or precipitation of the test item due to a re-action between the test item and the minerals in the test medium. Because the test item is a UVCB with several different components, the determination of the biological result was based on the nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.