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EC number: 433-460-1 | CAS number: 210880-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of an additional algal species
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 - 18 November, 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- March 2006
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples from two sampling intervals were received on November 14 (Day 0), and November 18, 2011 (Day 4). The nominal concentrations were Control, 2.56, 6.40, 16.0, 40.0 and 100 mg/L (ppm).
- Vehicle:
- no
- Details on test solutions:
- Highest Test Solution (100 mg a.i./L):
Nominal Test Solution Concentration: 100 mg a.i./L
Preparation Vessel: 1000-mL borosilicate glass volumetric flask
Amount of test material added to volumetric flask: 100.5 mg
Diluent: 1xAAP + 3x Silicate media filter sterilized with 0.20 µm filter
Resulting Solution Volume: 1L
Method of Mixing: Flask was sonicated for approximately 2 hours. The flask was intermittently shaken vigorously during this time to facilitate the sonication process. After sonication flask was inverted several times to complete mixing.
Test Solutions:
Preparation Vessels: 1000 mL volumetric flasks
Vessel Material: Borosilicate glass
Volume Prepared: 1000 mL
Preparation Day(s): Day 0 (as uniform batches)
Mixing Method: Inverted vessels several times
Media (Table 1): 1xAAP + 3x Silicate media filter sterilized with 0.20 µm filter
Media pH: 7.5 ± 0.1
Media Dilution Water: Distilled water blended
Nominal Test Levels (mg a.i../L) Total Volume Prepared Amount of Compound Added
Control (0) 1000 mL None
100 1000 mL 100.5 mg
The following test concentrations were made via serial dilution from the next higher test concentration. All vessels were brought to a volume of 1L with filter sterilized 1xAAP + 3x Silicate media after adding the appropriate volume of the next higher test solution.
Nominal Test Levels (mg a.i./L) Total Volume Prepared Volume of Next Higher Solution Added
40 1000 mL 400 ml
16 1000 mL 400 ml
6.4 1000 mL 400 ml
2.56 1000 mL 400 ml - Test organisms (species):
- Navicula pelliculosa
- Details on test organisms:
- Species Tested: Navicula pelliculosa
Source: University of Texas (UTEX), Austin, Texas
Date of Receipt: February 13, 2007
Batch Culture Preparation Date: November 11, 2011
Photoperiod: 24 hours Light
Light Intensity: 4300 lux (± 15%)
Temperature: 24 ± 2.0 °C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- Not specified
- Test temperature:
- 24.1 to 24.2 °C
- pH:
- 7.3 to 10.2
- Salinity:
- Not specified
- Conductivity:
- 157 to 176 µmhos/cm
- Nominal and measured concentrations:
- Nominal: 2.56, 6.4, 16, 40 and 100 mg a.i./L
Mean measured: 2.35, 6.0, 15, 37 and 95 mg a.i./L (ranged from 92 to 95% of nominal test concentrations) - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 40 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- The cells were observed each test day during the cell counting procedure with no abnormal observations noted.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the 96-hour EC50 and NOEC based on growth rate for N. pelliculosa were > 100 mg a.s./L and 40 mg a.s./L, respectively.
Reference
Please refer to "overall remark/ attached background material" field for result tables.
Table 1: Validity criteria for OECD 201 (2006)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
Factor of 72 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
25% |
yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests withPseudokirchneriella subcapitataandDesmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
1.9% |
yes |
Description of key information
From key study, the 96-h ErC50 for aquatic algae exposed to the present test material was > 100 mg a.s./L in freshwater.
From supportive study, the 96-h ErC50for aquatic algae exposed to the present test material was 37.8 mg a.s./L in saltwater.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC50 for marine water algae:
- 37.8 mg/L
- EC10 or NOEC for freshwater algae:
- 40 mg/L
- EC10 or NOEC for marine water algae:
- 6.4 mg/L
Additional information
Five GLP-studies are available for toxicity testing in aquatic algae and cyanobacteria and one of them was identified as key study.
In the key study (2012), the toxicity of test substance to aquatic algae was investigated in a 96-hours static test with N. pelliculosa according to guideline OECD 201. An initial cell density of 1.0 x 104cells/mL was achieved in each of the three replicates per test concentration. Algae were exposed to nominal test item concentrations of 2.56, 6.4, 16, 40 and 100 mg a.s./L, alongside with a control containing only test medium. Mean measured concentrations from days 0 and 4 ranged from 92 to 95 % of nominal concentrations. Based on the nominal concentration 96-h ErC50was > 100 mg a.s./L and NOEC (96 h) was determined to be 40 mg a.s./L.
There are four additional studies available, conducted with A. flos-aquae, S. capricornutum, S. costatum and S.subspicatus; the resulting ErC50values ranged from 37.8 to > 270 mg a.s./L. These studies were considered suitable for supporting purpose.
In the first supporting study (2012), the toxicity of test material to A. flos-aquae was determined in a 96-h semi-static test according to guideline OECD 201.The 96-hour ErC50 of the test substance was determined to be > 100 mg a.s./L based on the nominal concentrations.
In the second supporting study (2000), the toxicity of test material to S. capricornutumwas determined in a 96-h-static test according to US EPA OPP 123 guideline.The 96-hour ErC50 of the test substance was determined to be > 120 mg a.s./L, based on the mean measured concentrations.
In the third supporting study (2012), the toxicity of test material to S. costatum was determined in a 96-h-static test according to OECD 201 guideline. The 96-hour ErC50 and NOEC based on nominal concentration of the test substance were determined to be 37.8 and 6.4 mg a.s./L, respectively.
In the fourth supporting study (2000), the toxicity of test material to S. subspicatus was determined in a 120-h-static test according to OECD 201 guideline. The 120-hour ErC50 and ErC10 based on nominal concentration of the test substance were determined to be > 270 and 202.08 mg/L, respectively.
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