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EC number: 239-996-9 | CAS number: 15872-89-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Feb - 26 Feb 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- Comparable to guideline study with acceptable restrictions: Samples were not taken at 0 hours, nor were nominal concentrations reported. It was not stated if GLC analysis were performed by either direct aqueous injection or solvent extraction and subsequent analysis.
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute toxicities of organic chemicals to fathead minnows (Pimephales promelas) Vol 2
- Author:
- Geiger, D.L. et al.
- Year:
- 1 985
- Bibliographic source:
- Center for Lake Superior Environmental Studies, University of Wisconsin-Superior, Wisconsin, USA
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- two replicates tested with 20 fish each
- Deviations:
- yes
- Principles of method if other than guideline:
- Deviations: two replicates tested with 20 fish each
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Dibutyl adipate
- EC Number:
- 203-350-4
- EC Name:
- Dibutyl adipate
- Cas Number:
- 105-99-7
- Molecular formula:
- C14H26O4
- IUPAC Name:
- 1,6-dibutyl hexanedioate
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in study report): Dibutyl adipate
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken in all test concentrations.
- Sampling method: Samples were taken after 24 h and 72 h in replicate 1 and after 48 h and 96 h in replicate 2 of each test concentration.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Lake Superior water plus the test substance were added to the vessels and stirred vigorously.
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: fathead minnow
- Source: US-EPA Environmental Research Laboratory-Duluth
- Age at study initiation: 33 days
- Weight at study initiation: 0.32 g
- Method of breeding: Adults were held at 25 °C in flowing water with a 16 h light controlled photoperiod and fed frozen adult brine shrimp (Artemis spec.). Asbestos pipe was used as spawning substrate. Eggs were spawned and fertilized on the underside of these substrates. Substrates with embryos attached were removed to another 25 °C bath where hatching occurred.
- Feeding during test: Test fish were not fed 24 h before or during test.
ACCLIMATION
- Acclimation period: Fry of similar age (within 24 h) were transferred to flow-through rearing tanks at 25 °C with a 16h light photoperiod.
- Acclimation conditions: same as test
- Type and amount of food: Larvae were fed 40-48 h old brine shrimp nauplii
- Feeding frequency: two times daily (once on weekend days)
- Health during acclimation: Only groups of fish having healthy appearance were used for the test.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 47 mg/L as CaCO3
- Test temperature:
- 24.5 ± 0.46 °C
- pH:
- 7.51 ± 0.09
- Dissolved oxygen:
- 8.0 mg/L
- Nominal and measured concentrations:
- Nominal: not stated
Measured (arith. mean): 0.91, 1.43, 2.38, 4.70 and 9.09 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: fish tank
- Fill volume: 7.3 L
- Type of flow-through: cycling proportional diluter
- Renewal rate of test solution: 19.7 chamber volumes were added per day
- No. of organisms per vessel: 20
- No. of vessels per concentration: 2
- No. of vessels per control: 2
- Biomass loading rate: 0.044 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Lake Superior water which was filtered through sand and a FRAM filter.
- Metals: Zinc: 1.0 - 2.7 µg/L; Nickel: > 0.5 µg/L; Manganese: 0.2 - 11.5 µg/L; Lead: 7-20 µg/L; Iron: 2-83 µg/L; Cobalt: < 0.5 µg/L; Chromium: 2-20 µg/L; Cadmium: < 0.1 µg/L; Aluminium: 1-26 µg/L; Potassium: 0.48 - 0.59 mg/L; Magnesium: 2.9-3.6 mg/L; Calcium: 13.0-14.7 mg/L; Sodium: 1.1-1.2 mg/L
- Chlorine: 1.2 - 1.3 mg/L
- Alkalinity: 43.3 mg/L as CaCO3
- Ca/mg ratio: 3.6 - 5.0
- Conductivity: 78 - 86 µmhos/cm
- Intervals of water quality measurement: Temperature, dissolved oxygen, pH, total hardness and alkalinity were measured routinely. Temperature was monitored with a recording thermometer from which daily averages were calculated and recorded for a single tank. Dissolved oxygen was measured in low, medium, high concentration and control vessels at least once during the test. Total hardness and alkalinity were measured once during the test in the control and at low, medium and high test concentrations. The EDTA method (APHA, 1980) was used to determine hardness, while alkalinity was determined by potentiometric titration (APHA, 1980). The pH-value was measured once in high, medium, low and control treatments. Measurements were made with a meter, calibrated prior to each test.
OTHER TEST CONDITIONS
- Photoperiod: 16 h/8 h light/dark
- Light intensity: Illumination, provided by a fluorscent source, produced 28-48 lumens at the water surface.
EFFECT PARAMETERS MEASURED: Mortality and behavioral abnormalities were recorded after 24, 48 and 96 h test duration. Additionally, upon test termination, subsamples of control fish were weighed en masse. - Reference substance (positive control):
- yes
- Remarks:
- Numerous substances were tested using the same test design with same substances showing high toxicity
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.64 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: other: 3.39 - 3.90 mg/L
- Details on results:
- - Behavioural abnormalities: Affected fish swam near the bottom and lost equilibrium prior to death.
- Results with reference substance (positive control):
- Several substances showed to be very toxic to fish, such as Hexachloro-1,3-butadiene with LC50 = 0.09 mg/L or Chloromethyl Styrene with LC50 = 0.31 mg/L.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Measured concentration of the test substance
Test concentration
Measured tank concentrations [mg/L]
0h
24h
48h
72h
96h
average
Corrected average
Control (Replicate 1/2)
-
< 0.5 / -
-. / < 0.5
< 0.5 / -
- / < 0.5
< 0.5
< 0.5
Test conc. 1
(Replicate 1/2)
-
1.11 / -
- / 0.71
0.90 / -
- / 0.91
0.91
0.91
Test conc. 2
(Replicate 1/2)
-
1.92 / -
- / 1.19
1.20 / -
- / 1.44
1.44
1.43
Test conc. 3
(Replicate 1/2)
-
2.94 / -
- / 1.88
2.50 / -
- / 2.27
2.40
2.38
Test conc. 4
(Replicate 1/2)
-
5.78 / -
- / 3.75
4.76 / -
- / 4.62
4.73
4.70
Test conc. 5
(Replicate 1/2)
10.7 / -
- / 7.83
9.32 / -
- / 8.72
9.14
9.09
Table 2: Mortalities
Test concentration
Mortalities (initial number of fishes: 20 per replicate)
24h
48h
96h
Control (Replicate 1/2)
0 / 0
0 / 0
0 / 0
Test conc. 1
(Replicate 1/2)
0 / 0
0 / 0
0 / 0
Test conc. 2
(Replicate 1/2)
0 / 0
0 / 0
0 / 0
Test conc. 3
(Replicate 1/2)
0 / 0
0 / 0
0 / 0
Test conc. 4
(Replicate 1/2)
10 / 17
16 / 19
16 / 19
Test conc. 5
(Replicate 1/2)
20 / 20
20 / 20
20 / 20
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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