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EC number: 249-282-9 | CAS number: 28880-24-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25-06-2018 to 30-08-2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diisooctyl succinate
- EC Number:
- 249-282-9
- EC Name:
- Diisooctyl succinate
- Cas Number:
- 28880-24-2
- Molecular formula:
- C20H38O4
- IUPAC Name:
- bis(6-methylheptyl) succinate
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- The inoculum was derived from secondary effluent of a Sewage Treatment Plant. The inoculum was aerated and preconditioned for a period of 5 days at the temperature of 20°C. 1 ml per litre of the mineral medium was used to find out the optimum volume of the given effluent.
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Initial conc.:
- 1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 0.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 0.25 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- other: canola oil
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 91.67
- Sampling time:
- 28 d
- Details on results:
- Percentage Biodegradability of ‘Diisooctyl Succinate (SustOleo™ DCS)’ was found to be 16.75, 38.64, 77.60 and 91.67% at day 7, 14, 21 and 28, respectively, however Percentage Biodegradability of Reference item ‘Canola oil’ ’ was 92.0, 124.6, 127.2 and 119.8% on day 7, 14, 21 and 28, respectively. The viability of the inoculum and validity of the test were supported by the reference item canola oil, which degraded approximately 119.8 %. An average percent biodegradation of greater than 60% was achieved by day 7, thereby fulfilling the criteria for a valid test .
BOD5 / COD results
- Results with reference substance:
- 119.8 % after 28 day
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- substance is readily biodegradable according to OECD 301D
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