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EC number: 210-764-9 | CAS number: 623-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-02-11 - 1997-03-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- 4-bromobenzonitrile
- EC Number:
- 210-764-9
- EC Name:
- 4-bromobenzonitrile
- Cas Number:
- 623-00-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 4-bromobenzonitrile
- Reference substance name:
- 2-bromobenzonitrile
- EC Number:
- 218-045-1
- EC Name:
- 2-bromobenzonitrile
- Cas Number:
- 2042-37-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 2-bromobenzonitrile
- Reference substance name:
- 3-bromobenzonitrile
- EC Number:
- 230-127-9
- EC Name:
- 3-bromobenzonitrile
- Cas Number:
- 6952-59-6
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 3-bromobenzonitrile
- Reference substance name:
- Benzonitrile
- EC Number:
- 202-855-7
- EC Name:
- Benzonitrile
- Cas Number:
- 100-47-0
- Molecular formula:
- C7H5N
- IUPAC Name:
- benzonitrile
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Water
- Test material form:
- solid
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, Sulzfeld, GER
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: approx 6 w
- Weight at study initiation: within +/- 20 % of the sex mean
- Fasting period before study: over night prior to dosing until 3 - 4 h after adminstration
- Housing: 3 animals per sex per cage, polycarbonate cages, purified sawdust
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 5 d
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 °C
- Humidity (%): 50 %
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 1 % Aq.
- Doses:
- 200 mg/kg BW (m+f)
2000 mg/kg BW (m) - No. of animals per sex per dose:
- 3
- Control animals:
- no
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 200 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 2000 mg/kg bw: 1 m dead on day 2
1 m killed in extremis on day 4 - Clinical signs:
- other: 200 mg/kg bw: Hunched posture, uncoordinated movements 2000 mg/kg bw: Lethargy, ventro-lateral recumbency, hunched posture, uncoordinated movements, piloerection, swelling of the abdomen, pale skin, red staining of the periorbital region, ptosis of an eye
- Gross pathology:
- Enlarged adrenal glands, with hemorrhages, and thymis reduced in size were found in the male that was killed in extremis during the study.
Macroscopic post mortem examination of the other animal that died during the study and of the surviving animals at termination did not reval any abnormalities that are not occasionally noted among rats of this age and strain or were considered toxicologically significant.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The oral LD50 value of 4-Bromobenzonitrile in Wistar rats was established to be within the range of 200-2000 mg/kg bw.
According to GHS criteria 4-Bromobenzonitrile must be classified as hamful and requires the hazardous statement H302 "Harmful if swallowed". - Executive summary:
Assessment of the acute oral toxicity with 4 -Bromobenzonitrile in the rat (Acute Toxic Class Method).
The study was carried out based on the guidelines described in: EC Commission Directive 96/54/EC, Part B.1 tris "Acute Toxicity-Oral, Acute toxic Class Method" and OECD No. 423
"Acute Oral Toxicity - Acute toxic Class Method"
Initially, 4 -Bromobenzonitrile was adminstered by oral gavage th three m Wistar rats at 200 mg/kg bw. In a stepwise procedure additional groups of animals were dosed at 200 (f) and 2000 (m) mg/kg bw. All animals were subjected to daily observations ans weekly determination of bw. Macroscopic examination was performed on the day of death or after terminal sacrifice (day 15).
At a dose level of 2000 mg/kg bw one male was found dead on day 2 and another male was killed in extremis on day 4.
Clincal singns observed during the study period were as follows:
200 mg/kg bw: Hunched posture, uncoordinated movements
2000 mg/kg bw: Lethargy, ventro-lateral recumbency, hunched posture, uncoordinated movements, piloerection, swelling of the abdomen, pale skin, red staining of the periorbital region, ptosis of an eye.
Surviving animals have recovered from the symptoms by day 2 (200 mg/kg bw) or by day 14 (2000 mg/kg bw).
Surviving male dosed at 2000 mg/kg bw showed reduced bw gain over the first week, which improved over the 2nd week. The bw gain shown by the other survivig animals over the study period was considered to be similar to that expected of normal untreated anmals of the same age and strain.
Enlarged adrenal glands, with hemorrhages, and thymis reduced in size were found in the male that was killed in extremis during the study.
Macroscopic post mortem examination of the other animal that died during the study and of the surviving animals at termination did not reval any abnormalities that are not occasionally noted among rats of this age and strain or were considered toxicologically significant.
The oral LD50 value of 4-Bromobenzonitrile in Wistar rats was established to be within the range of 200-2000 mg/kg bw.
According to GHS criteria 4-Bromobenzonitrile must be classified as hamful and requires the hazard statement H302 "Harmful if swallowed".
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