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EC number: 210-764-9 | CAS number: 623-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-01-13 - 1997-02-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-bromobenzonitrile
- EC Number:
- 210-764-9
- EC Name:
- 4-bromobenzonitrile
- Cas Number:
- 623-00-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 4-bromobenzonitrile
- Reference substance name:
- 2-bromobenzonitrile
- EC Number:
- 218-045-1
- EC Name:
- 2-bromobenzonitrile
- Cas Number:
- 2042-37-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 2-bromobenzonitrile
- Reference substance name:
- 3-bromobenzonitrile
- EC Number:
- 230-127-9
- EC Name:
- 3-bromobenzonitrile
- Cas Number:
- 6952-59-6
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 3-bromobenzonitrile
- Reference substance name:
- Benzonitrile
- EC Number:
- 202-855-7
- EC Name:
- Benzonitrile
- Cas Number:
- 100-47-0
- Molecular formula:
- C7H5N
- IUPAC Name:
- benzonitrile
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Water
- Test material form:
- solid
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 16, 25, 40 mg/mL at start of exposure; 0,16, 25 mg/mL at the end of exposure
- Sampling method: 10 mL from approximate centre of test vessels + 10 mL reserve samples
- Sample storage conditions before analysis: deep-freeze
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The standard test procedures required generation of test solutions which contain completely dissolved test substance concentrations or stable and homogeneous mixtures or dispersions. The testing of concentrations that disturbed the test system were prevented (e.g. film of the test substance on the water surface).
All the test concentrations were prepared seperately, owing to the poor solubility of the test substance in the test medium. After pulverisation of the test substance, ultrasonic treatment and magnetic stirring were used alternately to accelerate suspending. In addition, the temperature was increased uo to 40 °C. Before introduction of the fish test solutions cooled to 22 °C. Final test solutions were all clear with test subtance particles present in the medium. The amount of test substance particles increased with increasing test concentration.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- - mean length
Range-finding: 2.97 +/- 0.31 cm
Final test: 2.4 +/- 1.4 cm
Mean weight:
Range-finding: 0.84 +/- 0.31 g
Final test: 0.44 +/- 0.05 g
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- ISO Medium
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 20.8 - 22.0 °C
- pH:
- 7.5 - 8.3
- Dissolved oxygen:
- 7.5 - 9.1
- Reference substance (positive control):
- yes
- Remarks:
- Pentachlorophenol (PCP)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. No mortality was observed in the control group. 2. Measured concentrations corrsepond with an estimated average exposure concentration of 60 % during the test. 3. Further, all test conditions remainded within the ranges prescribed by the protocol.
- Conclusions:
- Under the conditions of the present test 4-Bromobenzonitrile induced such severe effects at 40 mg/L and higher, that these effects had to be considered as lethal.
Based on nominal concentrations the 96 h LC50 was 32 mg/L. This LC50 value corresponded with actual average exposure concentration of 19 mg/L. - Executive summary:
96 h Acute Toxicity Study in Carp with 4 -Bromobenzonitrile.
The study procedures were based on the ECC directive 92/69, Part C.1. "Acute toxicity for fish": and the OECD guideline No. 203: "Fish Acute Toxicity Test", adopted 17 July, 1992.
The test was performed with carp exposed to concentrations ranging from 16 to 160 mg/L in a static system. 7 carp were exposed per concentration and a control. Samples for TOC-analysis were taken at the start and at the end of the test.
Test substance particles were present in all test solutions during the test. At the start of exposure the actual TOC recoveries ranged between 49 and 53 %. After 96 h of exposure, TOC recoveries had increased to approximatley 72 %. The increase of recoveries was probably caused by further dissolving of the test substance during the test. Hence, the actual average exposure concentrations were maintained an an astimated 60 % of nominal.
Under the conditions of the Present test 4 -Bromobenzonitrile induced no lethal effects in carp at or below 25 mg/L. The 96 h LC50 was 32 mg/L based on nominal concentrations with 0 % mortality at 25 mg/L and 100 % mortality at 40 mg/L. This LC50 value corresponded with an actual average concentration of 19 mg/L.
The "Bewertungszahl" is 4.5 based on the nominal LC50 and 4.7 based on the actual LC50.
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