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EC number: 240-968-3 | CAS number: 16919-19-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011/06/08 - 2012/02/21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2006/3/26
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ammonium hexafluorosilicate
- EC Number:
- 240-968-3
- EC Name:
- Ammonium hexafluorosilicate
- Cas Number:
- 16919-19-0
- Molecular formula:
- F6Si.2H4N
- IUPAC Name:
- diammonium hexafluorosilicate
- Test material form:
- solid: particulate/powder
1
- Specific details on test material used for the study:
- Ammoniumhexafiuorosilicate; Batch No.: GRA 2011-007; purity: 98.9 %.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from the freshly prepared test media (containing algae) of all test concentrations and from the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control (containing algae) were taken at the end of the test (after the 72 hours test period).
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Reconstituted Water (OECD Medium): Analytical grade salts were added at the following nominal concentrations in deionised water (conductivity < 5 µScm^-1):
NaHC03 50.0 mg/l
CaCl2 x 2 H2O 18.0 mg/l
NH4CI 15.0 mg/l
MgSO4 X 7 H2O 15.0 mg/l
MgCl2 X 6 H2O 12.0 mg/l
KH2PO4 1.6 mg/l
Na2EDTA x 2 H2O 100.0 µg/l
FeCl3 x 6 H2O 64.0 µg/l
MnCl2 x 4 H2O 415.0 µg/l
H3BO3 185.0 µg/l
Na2MoO4 x 2 H2O 7.0 µg/l
ZnCl2 3.0 µg/l
CoCl2 x 6 H2O 1.5 µg/l
CUC12 x 2 H2O 0.01 µg/l
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata, Strain No. 61.81 SAG
cultivated in the laboratories of IBACON; original source: "Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen", 37073 Göttingen, Germany
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
Test conditions
- Test temperature:
- 23 °C
- pH:
- 8.1 (start)
8.5-8.9 (end) - Nominal and measured concentrations:
- 0, 1, 3.2, 10, 32, 100 mg/l
- Details on test conditions:
- Erlenmeyer flasks of 50 mL volume with 50 mL of test medium.
Continuous illumination
The light intensity was measured once during the test at 6 positions distributed over the experimental area at the surface of the test media.
Mean light intensity: 6362 lux (range: 6110 to 6730 lux)
Test conditions were recorded with suitable instruments and documented in the raw data.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- ca. 75.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 22.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Reported statistics and error estimates:
- none
Any other information on results incl. tables
The quantification of the ammonium of the test item was performed using ion chromatography with conductivity detection (IC-CD). At the start of the test 119 % of the nominal test concentration was found (mean value from test concentrations of nominal 10 to 100 mg/L). In the aged test media a recalculation from the ammonium concentration to the corresponding concentration of test item was not performed, since the results were not reasonable. Perhaps the high algae density in the aged test media influences the ammonium concentration or its determination.
Since correct dosing was demonstrated, all reported results refer to nominal concentrations.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The influence of Ammoniumhexafluorosilicate on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour ErC50 value was calculated to be > 100 mg test item/L and the 72-hour EyC50 was calculated to be > 100 mg test item/L. The 72-hour NOErC was determined to be 10 mg test item/L and the associated 72-hour LOErC is 32 mg test item/L. The 72-hour NOEyC was determined to be 3.2 mg test item/L and the associated 72-hour LOEyC is 10 mg test item/L.
- Executive summary:
The purpose of this test was to determine the inhibitory effect of the test item Ammoniumhexafiuorosilicate on the growth of the freshwater green algae Pseudokirchneriella subcapitata. Exponentially growing cultures of this unicellular green algal species were exposed to various concentrations of the test item under defined conditions. The inhibition of growth in relation to control cultures was determined over a test period of 72 hours, and thus over several algal generations.
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