Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 255-003-1 | CAS number: 40615-39-2
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-01-15 to 2018-02-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Version / remarks:
- 27 Jul 1995
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 830.7840 (Water Solubility)
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- GLP compliance:
- no
- Type of method:
- column elution method
- Key result
- Water solubility:
- ca. 3 mg/L
- Conc. based on:
- test mat. (dissolved fraction)
- Incubation duration:
- 2 h
- Temp.:
- 20 °C
- pH:
- 7.1
- Conclusions:
- The water solubility of 5’-O-(4,4’-Dimethoxytrityl)thymidine in double distilled water was found to be 3.0 mg/L at pH 7.1 and a temperature of 20 °C.
- Executive summary:
The water solubility of 5’-O-(4,4’-Dimethoxytrityl)thymidine was determined in double distilled water according to the "column elution method" described in the European Commission Council regulation (EC) No 440/2008, Annex, Part A, A.6., OECD-guideline 105 and the EPA Product Properties Test Guideline OCSPP 830.7840 and found to be 3.0 mg/L at pH 7.1 and a temperature of 20 °C.
Reference
RUN 1 – 25 mL/h: Regarding fractions 06 to 15 the solubility varied between 1.659 mg/L and 1.960 mg/L. Average solubility: 1.832 mg/L (RSD: 4.8 %).
Table 1: Column elution method, Run 1 (25 mL/h); Test material amount used for coating: 54.38 mg
| Fraction no. | Approx. volume (mL) | pH | Cs (mg/L) |
| Fraction 06 | 14.0 | 7 .6 | 1.659 |
| Fraction 07 | 15.0 | 7.1 | 1.854 |
| Fraction 08 | 14.0 | 6.9 | 1.731 |
| Fraction 09 | 15.0 | 7.0 | 1.778 |
| Fraction 10 | 16.0 | 7.1 | 1.875 |
| Fraction 11 | 15.0 | 7.2 | 1.837 |
| Fraction 12 | 15.0 | 7.3 | 1.960 |
| Fraction 13 | 14.0 | 6 .9 | 1.842 |
| Fraction 14 | 16.0 | 7.0 | 1.856 |
| Fraction 15 | 14.0 | 7.2 | 1.924 |
RUN 2 – 12.5 mL/h: Regarding fractions 06 to 15 the solubility varied between 2.789 mg/L and 3.027 mg/L. Average solubility: 2.884 mg/L (RSD: 2.2 %).
Table 2: Column elution method, Run 2 (12.5 mL/h); Test material amount used for coating: 51.37 mg
| Fraction no. | Approx. volume (mL) | pH | Cs (mg/L) |
| Fraction 06 | 15.0 | 7.1 | 2.871 |
| Fraction 07 | 15.0 | 6.9 | 2.927 |
| Fraction 08 | 15.0 | 7.1 | 3.027 |
| Fraction 09 | 15.0 | 7.1 | 2.887 |
| Fraction 10 | 15.0 | 7.0 | 2.824 |
| Fraction 11 | 15.0 | 7.5 | 2.860 |
| Fraction 12 | 15.0 | 7.1 | 2.907 |
| Fraction 13 | 15.0 | 7.0 | 2.789 |
| Fraction 14 | 15.0 | 7.1 | 2.884 |
| Fraction 15 | 15.0 | 7.1 | 2.867 |
Run 3 - 6.25 mL/h: Regarding fractions 06 to 15 the solubility varied between 3.055 mg/L and 3.270 mg/L. Average solubility: 3.147 mg/L (RSD: 2.2 %).
Table 3: Column elution method, Run 3 (6.25 mL/h); Test material amount used for coating: 52.78 mg
| Fraction no. | Approx. volume (mL) | pH | Cs (mg/L) |
| Fraction 06 | 10.0 | 7.7 | 3.083 |
| Fraction 07 | 10.0 | 7.1 | 3.096 |
| Fraction 08 | 10.0 | 7.0 | 3.094 |
| Fraction 09 | 11.0 | 7.0 | 3.120 |
| Fraction 10 | 11.0 | 7.0 | 3.055 |
| Fraction 11 | 11.0 | 7.0 | 3.139 |
| Fraction 12 | 10.0 | 7.0 | 3.226 |
| Fraction 13 | 11.0 | 6.8 | 3.195 |
| Fraction 14 | 11.0 | 6.6 | 3.270 |
| Fraction 15 | 10.0 | 6.9 | 3.189 |
The determined water solubilities for RUN 1 and RUN 2 show a repeatability that was significantly higher than 30 %. In addition to this the found water solubility for the lower flow rate is higher than the solubility for the higher flow rate. This is an indication that equilibrium of the aqueous phases was not yet achieved at the highest flow rate. The results for the two lower flow rates (RUN 2 and RUN 3) show a repeatability, that is < 30 %, therefore the results of these two runs are considered to be in accordance. At the end of each RUN, there was still enough test item left on the surface of the glass beads.
Description of key information
The water solubility of 5’-O-(4,4’-Dimethoxytrityl)thymidine in double distilled water was found to be 3.0 mg/L at pH 7.1 and a temperature of 20 °C.
Key value for chemical safety assessment
- Water solubility:
- 3 mg/L
- at the temperature of:
- 20 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
