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EC number: 254-599-0 | CAS number: 39711-79-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenic investigation of test chemical in the Salmonella/mammalian microsome test
- Author:
- Pia Haubro Andersen and et. al.
- Year:
- 1 984
- Bibliographic source:
- Mutation Research (1984)
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Salmonella Mutagenicity Tests of test chemical was performed by Bacterial gene mutation assay
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- trans-menthone
- EC Number:
- 201-941-1
- EC Name:
- trans-menthone
- Cas Number:
- 89-80-5
- Molecular formula:
- C10H18O
- IUPAC Name:
- (2S,5R)-2-Isopropyl-5-methylcyclohexanone
- Test material form:
- solid
- Details on test material:
- IUPAC name:2-Isopropyl-5-methylcyclohexanone
Mol. formula: C10H18O
Molecular Weight: 154.2512
Smiles: C1([C@@H](CC[C@@H](C1)C)C(C)C)=O
InChI: 1S/C10H18O/c1-7(2)9-5-4-8(3)6-10(9)11/h7-9H,4-6H2,1-3H3
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- No data available
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data available
- Metabolic activation:
- with and without
- Metabolic activation system:
- (The S-9 fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared)
- Test concentrations with justification for top dose:
- 0.00, 6.4, 32, 160, 800 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Test material soluble in DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- other: 2-anthramine for all strains with S9 metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
Standard plate
DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: 5 plate
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available-
Determination of endoreplication: No data available-
Other: No data available
OTHER: No data available - Rationale for test conditions:
- No data available
- Evaluation criteria:
- The histidine-revertant (his') colonies arising on the plates were counted
- Statistics:
- Mean number of revertants for n mumber of plates for each dose level was calculated to be the square value of mean(y) of squared roots. Standard error of mean was calculated
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535 ,TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Remarks:
- DMSO
- Untreated negative controls validity:
- valid
- Remarks:
- DMSO
- Positive controls validity:
- valid
- Additional information on results:
- No data available
Any other information on results incl. tables
Dose µg/plate |
TA1535 |
TA100 |
TA1538 |
TA98 |
|||||
Without S9 |
With S9 |
Without S9 |
With S9 |
Without S9 |
With S9 |
Without S9 |
With S9 |
||
0.00 |
7±1 |
8±1 |
85±4 |
114±8 |
7±1 |
11±1 |
32±3 |
33±2 |
|
6.4 |
7±1 |
10±1 |
96±4 |
114±8 |
13±1 |
11±1 |
25±2 |
28±2 |
|
32 |
7±1 |
9±1 |
92±4 |
121±8 |
12±2 |
12±1 |
31±3 |
27±2 |
|
160 |
5±1 |
8±1 |
97±4 |
123±8 |
8±2 |
11±1 |
28±2 |
26±2 |
|
800 |
7±1 |
9±1 |
69±6 |
104±7 |
4±1 |
11±1 |
13±3 |
22±2 |
|
Sodium Azide |
499±20 |
|
361± 20 |
|
|
|
|
|
|
2-Anthramine |
|
169± 7 |
|
278 ±12 |
|
119± 6 |
|
233± 13 |
|
2-Nitrofluorene |
|
|
|
|
38± 3 |
|
186± 13 |
|
Applicant's summary and conclusion
- Conclusions:
- Salmonella Mutagenicity Tests of test chemical was performed in Salmonella strain TA98, TA100, TA1535, and TA 1537 did not produce mutation, therefore it is considered to be negative for gene mutation in vitro.
- Executive summary:
SalmonellaMutagenicity Tests of test chemical was performed in Salmonella strainTA98, TA100, TA1535, and TA 1537. Both in the presence and absence of S9 metabolic activation system.
The test chemical was dissolved in DMSO to made dose concentration of 0.00, 6.4, 32, 160, 800 µg/plate in 5 parallel plates.The S-9 fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared.However 2-anthramine served positive control with S9 fraction for all strain of Salmonella typhimurium and sodium azide (TA1535 and TA 100), 2-nitrofluorene (TA 1538 and TA98) served as positive control without S9 fraction.
Mutagenicity evaluated by counting histidine-revertant (his') colonies arising on these plates. As test chemical did not produce mutationin Salmonella strainTA98, TA100, TA1535, and TA 1537,Therefore itis considered to be negative for gene mutation in vitro.
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