Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 243-411-2 | CAS number: 19894-99-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented publication which meets basic scientific principles
Data source
Reference
- Reference Type:
- publication
- Title:
- Degradation of α-pinene oxide and [2H7]-2,5,6-trimethyl-hept-(2E)-enoic acid by Pseudomonas fluorescens NCIMB 11761
- Author:
- Zorn H, Neuser F, Berger RG
- Year:
- 2 004
- Bibliographic source:
- Journal of Biotechnology 107, 255-263
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Batches of α-pinene oxide added every 15 minutes to a 100 ml cell suspension of Pseudomonas fluorescens for 3 or 5 hours. Sampling was carried out shortly before the test substance was added on each occasion, with the presence of metabolites (and the unconverted substrate) measured by gas chromatography.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- [1R-(1α,2β,4β,6α)]-2,2,7-trimethyl-3-oxatricyclo[4.1.1.02,4]octane
- EC Number:
- 243-411-2
- EC Name:
- [1R-(1α,2β,4β,6α)]-2,2,7-trimethyl-3-oxatricyclo[4.1.1.02,4]octane
- Cas Number:
- 19894-99-6
- Molecular formula:
- C10H16O
- IUPAC Name:
- (1R,2R,4S,6R)-2,7,7-trimethyl-3-oxatricyclo[4.1.1.0^{2,4}]octane
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): (-)-α-pinene oxide- Substance type: no data- Physical state: no data- Analytical purity: no data- Impurities (identity and concentrations): no data- Composition of test material, percentage of components: no data- Isomers composition: no data- Purity test date: no data- Lot/batch No.: no data - Expiration date of the lot/batch: no data- Stability under test conditions: no data- Storage condition of test material: no data
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Pseudomonas fluorescens NCIMB 11761
- Duration of test (contact time):
- >= 3 - <= 5 h
Initial test substance concentrationopen allclose all
- Initial conc.:
- 152 mmol/L
- Based on:
- other: total of 12 additions of 12.7 mmol/L provided at 15 minute intervals over a 3-hr period
- Initial conc.:
- 571 mmol/L
- Based on:
- other: total of 18 additions of 31.7 mmol/L provided at 15 minute intervals over a 5-hr period
Results and discussion
% Degradationopen allclose all
- Parameter:
- other: aldehyde yield
- Value:
- ca. 56
- Sampling time:
- 3 h
- Remarks on result:
- other: without Lewatit
- Parameter:
- other: aldehyde yield
- Value:
- ca. 67
- Sampling time:
- 3 h
- Remarks on result:
- other: with Lewatit
- Parameter:
- other: aldehyde yield
- Value:
- ca. 63
- Sampling time:
- 5 h
- Remarks on result:
- other: without Lewatit
- Parameter:
- other: aldehyde yield
- Value:
- ca. 66
- Sampling time:
- 5 h
- Remarks on result:
- other: with Lewatit
- Details on results:
- In the 3-hour experiment, a continuous accumulation of isonovalal started after the second dose, and substrate concentration remained at a low steady state level (of about 2 g/L, about 12.7 mmol/L). The concentration of novalal increased slowly from 0 to almost 1 g/L at 3 hours. Other volatiles were found in traces. [Similar data is not provided for the 5-hour experiment.]
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- other: rapidly degraded under test conditions
- Conclusions:
- In a non-guideline study, L-pinene alpha epoxide was very rapidly degraded [removal of the epoxide group at least] following a 3- or 5-hour incubation with Pseudomonas fluorescens.
- Executive summary:
In a good-quality, non-guideline study, the degradation of L-pinene alpha epoxide has been evaluated in Pseudomonas fluorescens.
L-pinene alpha epoxide was added in regular batches to a 100 ml bacterial cell suspension [bacterial concentration not given]. Shortly before this, 1 ml samples were taken for analysis by gas chromatography. In a 3-hour test, 1.27 mmol was added every 15 minutes (total dose 15.24 mmol). A similar 5-hour test was conducted, in which 3.17 mmol was added every 15 minutes (total dose 57.06 mmol). Each test was conducted with and without Lewatit, which was used to collect metabolites before conversion to substances not amenable to gas chromatography.
In the 3-hour test, 56% and 67% of the administered dose was recovered as volatile aldehydes, with and without Lewatit, respectively. In the 5-hour test, recovery was 63% and 66% with and without Lewatit. The key detected metabolite was isonovalal [predicted as readily biodegradable in EPISuite], while levels of novalal and trace volatiles were also seen to increase.
Under the conditions of this study, L-pinene alpha epoxide was very rapidly degraded [removal of the epoxide group at least] when incubated with P. fluorescens.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.