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EC number: 223-529-0 | CAS number: 3943-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 August 2016 to 29 September 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl 3,4-dihydroxybenzoate
- EC Number:
- 223-529-0
- EC Name:
- Ethyl 3,4-dihydroxybenzoate
- Cas Number:
- 3943-89-3
- Molecular formula:
- C9H10O4
- IUPAC Name:
- ethyl 3,4-dihydroxybenzoate
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands
- Storage conditions: preconditioned to experimental conditions by continuous aeration in the climate controlled room
- Storage length: approximately 26 hours
- Preparation of inoculum for exposure: pre-conditioned to expermental conditions
- Pretreatment: allowed to settle, the supernatant is used as inoculum
- Concentration of sludge: 10 mL/L medium
- suspended solids: 3.7 g/L - Duration of test (contact time):
- 29 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to the guideline
- Test temperature: 21.7 - 22.4°C.
- pH: 7.5-7.6
- pH adjusted: no
- Aeration of dilution water: yes but interrupted on day 13 (<1 day)
- Suspended solids concentration: 3.7 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 litre brown glass vessels
- Number of culture flasks/concentration: 2 for inoculum controls, 2 for test substance, 1 for reference substance, 1 for toxicity control
- Method used to create aerobic conditions: Synthetic air was sparged through the solutions
- Measuring equipment: A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was sparged through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Test performed in closed vessels due to significant volatility of test substance: NA
- Details of trap for CO2: 3 BaOH traps in serie
SAMPLING: day 2, 5, 7, 9, 12, 13, 14, 19, 23, 27, 29 (+after elution with HCl)
STATISTICAL METHODS: none applied
Reference substance
- Reference substance:
- acetic acid, sodium salt
- Remarks:
- 40 mg/L, 12 mg/L as TOC
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 8 - <= 9
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 61 - <= 68
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 65 - <= 94
- Sampling time:
- 29 d
- Details on results:
- Both test bottles reach >60% biodegradation within the 10 day window. Therefore, it was concluded that the >20% difference in duplicates does not interfere with the validity of this test.
Any other information on results incl. tables
CO2production and percentage biodegradation
Day |
HCl (0.05 N) titrated (mL) |
Cumulative CO2 (mg) Bottle A |
Cumulative CO2 (mg) Bottle B |
Biodegradation1) (%) Bottle A |
Biodegradation1) (%) Bottle B |
||
Blank (mean) |
Bottle A |
Bottle B |
|||||
2 |
47.07 |
45.80 |
46.10 |
1.4 |
1.1 |
2 |
1 |
5 |
47.08 |
44.55 |
44.96 |
4.2 |
3.4 |
5 |
4 |
7 |
46.71 |
44.26 |
42.61 |
6.9 |
7.9 |
8 |
9 |
9 |
47.02 |
40.23 |
24.61 |
14.3 |
32.5 |
16 |
37 |
12 |
47.11 |
15.75 |
33.27 |
48.8 |
47.8 |
55 |
54 |
13 |
48.41 |
41.56 |
44.18 |
56.4 |
52.4 |
63 |
59 |
14 |
49.10 |
45.28 |
47.82 |
60.5 |
53.8 |
68 |
61 |
19 |
47.76 |
37.46 |
46.97 |
71.9 |
54.7 |
81 |
62 |
23 |
46.70 |
41.74 |
45.68 |
77.3 |
55.8 |
87 |
63 |
27 |
44.37 |
40.86 |
43.31 |
81.2 |
57.0 |
91 |
64 |
29 |
46.99 |
45.13 |
46.85 |
83.2 |
57.1 |
93 |
64 |
29 |
49.45 |
48.88 |
49.67 |
83.8 |
57.1 |
94 |
64 |
29 |
51.26 |
51.57 |
51.11 |
83.8 |
57.3 |
94 |
65 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Remarks:
- the deviation between the 2 replicates exposed to the test substance was > 20%, as both replicates showed ready biodegradation, this finding is considered not to interfere with the validity of the test.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is considered readily biodegradable under the conditions of the test
- Executive summary:
In a CO2 -evolution test, the substance degraded for > 60% (actually 94 and 65% per replicate) during the 29 day test period. As both of the replicates fulfilled the 10-day window criterion, the results are sufficient valid to conclude that the substance is readily biodegradable.
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