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EC number: 205-503-0 | CAS number: 141-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Succinic acid may be used as an analogue for malonic acid, based on the following considerations. Firstly, dicarboxylic acids are naturally occurring metabolic products of fatty acid oxidation, and are rapidly beta-oxidised. A category approach for short-medium chain dicarboxylic acids, including malonic acid and succinic acid, has been validated and used by various bodies including the Cosmetics Ingredient Review Panel and the European Food Safety Authority.Disodium succinate may be used as an analogue for succinic acid as there is no significant difference in toxicity expected between succinic acid and disodium succinate, since both substances will dissociate into succinate ion under physiological conditions.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Disodium succinate hexahydrate (CAS No.: 6106-21-4, Nacalai Tesque, Inc. (Japan), Lot. No.: MOT9476, Purity = 100.2%, Molecular Weight = 270.14
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical Procedure: The tested concentrations were measured at the start and the end of the test period using HPLC.
- Vehicle:
- no
- Details on test solutions:
- Vehicle/Solvent and Concentrations: Any solvent was not used.Stock Solutions Preparations and Stability: Test chemical was refrigerated. The stability of the chemical was confirmed by IR spectrum.The IR spectrum at the end of the test was same at the start of test. Dilution Water Source: Dilution water was prepared from tap water (Kurume city, Japan). The tap water was dechlorinated and treated by ctivated carbon. After that Residual Chlorine was removed from the water. Before using the dilution water, aeration was fully carried out.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- Supplier: Test organisms were obtained from Nakajima Yougyo-jo (Private Fish Farm, Japan), before one month and a half of a test.Size (length and weight): 2.3 cm (2.2 - 2.4 cm) in length; 0.16 g (0.13 - 0.23 g) in weightAge: Not describedTest organisms were acclimated for 28 days before testing. During acclimation, test fishes were fed with TETRAMINE equivalent to 3% of weight per day. These test organisms were not fed for 24 hours before the test started. The mortality of the test organisms for 7 days before testing was less than 5%.
- Test type:
- flow-through
- Water media type:
- freshwater
- Hardness:
- Total hardness (as CaCO3): = 61.0 mg/L
- Test temperature:
- Water temperature were measured for old and renewal solution with control and each concentration at the start of test and every 24 hours.Water Temperature: 24.1 - 24.9ºC
- pH:
- Water chemistrywas measured for old and renewal solution with control and each concentration at the start of test and every 24 hours.pH: 7.6 - 7.8Water Temperature: 24.1 - 24.9ºC
- Dissolved oxygen:
- Water chemistrywas measured for old and renewal solution with control and each concentration at the start of test and every 24 hours.DO: 7.5 - 8.3 mg/L
- Nominal and measured concentrations:
- Nominal Concentrations: control, 6.25, 12.5, 25.0, 50.0 and 100 mg/L
- Details on test conditions:
- Light Condition: 16:8 hours, light-darkness cycleFeeding: NoneAeration : Test solution was not aerated during the test period.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 95.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 47 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 95.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- None of the test organisms were killed during exposure period at control, 6.25, 12.5, 25.0, 50.0 mg/L. The lowest concentration from which the test organisms were killed was 100mg/L at 96 h.Other Effect: Toxicological symptoms were not observed at any concentration.
- Results with reference substance (positive control):
- LC50(96 hr) for a reference substance (copper sulfate pentahydrate) was 0.707 mg/L.
- Reported statistics and error estimates:
- Data Analysis: During test period the test organismss motality was less than 50% in all concentrations, therefore the LC50 is more than the highestconcentration.Method of Calculating Mean Measured Concentrations (i.e. arithmetic mean, geometric mean, etc.): Arithmetic mean
- Sublethal observations / clinical signs:
Cumulative Mortality
Measured Conc. mg/L
Cumulative Number of Dead (Percent Mortality)
24hr
48hr
72hr
96hr
Control
0 (0)
0 (0)
0 (0)
0 (0)
5.64
0 (0)
0 (0)
0 (0)
0 (0)
11.6
0 (0)
0 (0)
0 (0)
0 (0)
23.6
0 (0)
0 (0)
0 (0)
0 (0)
47.0
0 (0)
0 (0)
0 (0)
0 (0)
95.4
0 (0)
0 (0)
0 (0)
1 (10)
Measured Concentrations: The tested concentrations were measured at 0 and 96 hour. For all of them, the deviations from the nominal concentration were less than +/-20%.
Nominal conc. mg/L
Measured Conc., mg/L
Mean* mg/L
Percent of nominal
0 Hour
96 Hour
0 Hour
96 Hour
Control
<0.600
<0.600
---
---
---
6.25
6.14
5.13
5.64
98.2
82.1
12.5
12.2
10.9
11.6
97.6
87.4
25.0
24.3
22.8
23.6
97.3
91.3
50.0
46.6
47.3
47.0
93.2
94.6
100
94.8
96.1
95.4
94.8
96.1
*: Mean measured concentration (Arithmetic mean)
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 96 h acute toxicity study of disodium succinate hexahydrate with fish (OECD TG 203, Oryzias latipes, flow-through), the study recorded a 96 h LC0 of 47.0 mg/L (equivalent to 28.2 mg/L disodium succinate), 96 h LC50 > 95.4 mg/L (equivalent to 57.2 mg/L disodium succinate) and 96 h LC100 > 95.4 mg/L (equivalent to 57.2 mg/L disodium succinate). In this test, only one individual out of twenty died at the highest concentration of 95.4 mg/L.
- Executive summary:
In a 96 h acute toxicity study of disodium succinate hexahydrate with fish (OECD TG 203, Oryzias latipes, flow-through), the study recorded a 96 h LC0 of 47.0 mg/L (equivalent to 28.2 mg/L disodium succinate), 96 h LC50 > 95.4 mg/L (equivalent to 57.2 mg/L disodium succinate) and 96 h LC100 > 95.4 mg/L (equivalent to 57.2 mg/L disodium succinate).. In this test, only one individual out of twenty died at the highest concentration of 95.4 mg/L.
Reference
Description of key information
In a 96 h acute toxicity study of disodium succinate hexahydrate with fish (OECD TG 203, Oryzias latipes, flow-through), the study recorded a 96 h LC0 of 47.0 mg/L (equivalent to 28.2 mg/L disodium succinate), 96 h LC50 > 95.4 mg/L (equivalent to 57.2 mg/L disodium succinate) and 96 h LC100 > 95.4 mg/L (equivalent to 57.2 mg/L disodium succinate).. In this test, only one individual out of twenty died at the highest concentration of 95.4 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 57.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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