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EC number: 692-061-0 | CAS number: 1207435-39-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Data waiving:
- other justification
- Justification for data waiving:
- the study does not need to be conducted because a long-term aquatic toxicity study on invertebrates is available
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- No full documentation available
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes
- Details on sampling:
- The number of immobilized Daphnia was recorded at 24 and 48 h.
- Vehicle:
- no
- Details on test solutions:
- Control and five test concentrations. Concentration range: 50 - 191 mg/L.
Volume: 20 mL in glass beakers. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Neonates (< 24 h old)
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1 °C
- Nominal and measured concentrations:
- Control and five test concentrations. Concentration range: 50 - 191 mg/L
- Details on test conditions:
- Illumination: 16 h light and 8 h dark cycle, 4000K, 600–800 lx.
Other incubation conditions: daphnids were not fed during the test.
The test was performed in glass beakers with 20 mL of the test solution. There were 5 neonates in each beaker. Control had 4 replicates and the five test concentrations 2 replicates. - Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 118 mg/L
- Conc. based on:
- test mat.
- Reported statistics and error estimates:
- n= 4, 95% confidence limit: lower = 76 mg/L and upper =183 mg/L.
- Conclusions:
- Fast pyrolysis bio-oil (BTG) showed low toxicity to Daphnia (EC50, 48 h = ca. 118 mg/L)
- Executive summary:
Acute toxicity of the test material (BTG, commercial pine wood based fast pyrolysis bio-oil) to Daphnia manga was tested according to OECD guideline no. 202 (2004) and in compliance with GLP. Five test concentrations were prepared. Test vessels with the daphnids were incubated for 48h and the number of immobilized D. magna was recorded at 24 and 48h, and the EC50 was calculated. According to the immobilization test, fast pyrolysis bio-oil from pine wood showed low toxicity to Daphnia, as the determined 48 h EC50 value was > 100 mg/L (EC50daphnia = 118 mg/L).
The study is reliable with restrictions as full documentation is not available due to type of information (publication).
Referenceopen allclose all
Description of key information
Long term toxicity of Fast Pyrolysis Bio-oils (WAF) to aquatic invertebrates was studied instead of short term toxicity. In a 21-d Daphnia toxicity (reproduction) study with the test material, EC50 for mortality at 21 days was found to be > 39.7 mg/L (the highest concentration studied; loading rate 100 mg/L) (Zawadsky, 2013). EC50 (48 h) was > 39.7 mg/L as well.
In a 48 h immobilization test (OECD guideline no. 202 [2004]: "Daphnia sp., Acute Immobilisation Test") published in literature, pine wood based Fast Pyrolysis Bio-oil was of low toxicity to Daphnia magna (EC50 ca. 118 mg/l).
Key value for chemical safety assessment
Additional information
Due to poor water solubility of the substance, the organisms were exposed to a Water Accommodated Fraction (WAF) of the test substance. This is a commonly accepted procedure for regulatory studies on poorly water soluble substances.
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