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EC number: 947-719-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-07-12 - 2017-07-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- other: EPA Health Effects Test Guidelines, OCSPP 850.1010
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch/Lot Number: 7675229
Description: White/gray solid
Purity: 100%
Expiry date: 31 August 2018 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L solutions
- Sampling method: Duplicate samples taken at t=0 ad t=48h in plastic tubes.
- Sample storage conditions before analysis: Frozen and kept at approx. -20 deg C at the test facility. One set of samples sent to the test site for analysis and one set retained as back-up at the test facility. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
addition of the test item, mixed into the test medium (ISO Medium) using ultrasonic bath (5 minutes). As a Limit test was carried out, further dilution of stock solution was not performed. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: Daphnia magna
- Justification for species other than prescribed by test guideline: standard species for acute immobilisation test
- Source: István Szent University, 2100 Gödöllő, Páter Károly u. 1, Hungary
- Breeding: The Daphnia are bred in Ecotoxicological Laboratory of CiToxLAB Hungary Ltd. The health of the stock animals is continuously monitored by visual daily checking. Abnormal behavior or significant decrease of population is recorded.
- Number of animals: There were 20 animals in test group and control group respectively, divided into 4 replicates (5 animals / replicate)
- Age of the animals: They were less than 24 h old at the beginning of the test
ACCLIMATION
- Acclimation period: There was no acclimatization because the water used was similar to the culture water.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- The reconstituted water (ISO medium) had a total hardness of 248 mg/L (as CaCO3).
- Test temperature:
- 20.2 – 20.6°C
- pH:
- 7.39 – 7.62
- Dissolved oxygen:
- 7.5 – 8.5 mg/L
- Salinity:
- Not reported
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- Nominal: 100 mg/L
Measured: 105.3 mg/L at the start and 102.9 mg/L at the end of the experiment.
As the measured concentration deviated not more than 20 per cent from the nominal, biological results are based on the nominal concentration. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4 (main test)
- No. of vessels per control (replicates): 4 (main test)
- Biomass loading rate: 8 mL test solution/animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (ISO medium, according to OECD 202) was used as dilution water for both the range finding and definitive tests. The same composition of reconstituted water was used for the tests and for breeding the test animals.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light/ 8 h dark
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The number of immobilised animals and the percentage of immobility were determined at 24 and 48 hours.
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10, 100 mg/L nominal
- Results used to determine the conditions for the definitive study:
1/10 animals immobilised in control and 0.1 mg/L solutions. 0/10 animals immobilised in 1, 10, 100 mg/L solutions.
Based on these results it was decided to perform a limit test at 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobility was observed in any of the controls or animals tested at 100 mg/L at 24 or 48 hours.
In addition to immobility, no abnormal behaviour or appearance of test animals was detected. - Results with reference substance (positive control):
- The date of the last study (Study Code: 17/058-023DA) with reference item Potassium dichromate (batch no.: A0345704) is: 08 - 09 March 2017.
The 24h EC50: 0.64 mg/L, (95 % confidence limits: 0.59 – 0.68 mg/L) - Reported statistics and error estimates:
- No statistical analysis was performed because of the lack of toxic effects.
The EC50, NOEC, LOEC and EC100 values were determined directly from the raw data. - Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were within acceptable limits and therefore the study can be considered as valid.
- Conclusions:
- The 24h and 48h EC50 value: > 100 mg/L (nominal)
The 48h No-Observed Effect Concentration (NOEC): 100 mg/L (nominal) - Executive summary:
Acute toxicity of the test item on Daphnia magna was assessed in an Acute immobilisation test, over an exposure period of 48 hours in a static test system (OECD TG 202).
Because no toxic response was observed during the preliminary range-finding test, a Limit Test was carried out using only one test concentration (100 mg/L) and one control group in the definitive test.
The test concentration was analytically determined at the start and at the end of the experiment. Measured concentration was 105.3 mg/L at the start and 102.9 mg/L at the end of the experiment.
As the measured concentration deviated not more than 20 per cent from the nominal, biological results are based on the nominal concentration.
Twenty animals, divided into four groups (glass beaker) of five animals each were used at the test concentration and for the control.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of the test item were as follows:
The 24h and 48h EC50 value: > 100 mg/L (nominal)
The 48h EC100 value: > 100 mg/L (nominal)
The 48h No-Observed Effect Concentration (NOEC): 100 mg/L (nominal)
The 48h Lowest Observed Effect Concentration (LOEC): > 100 mg/L (nominal)
Reference
Description of key information
The 24h and 48h EC50 value: > 100 mg/L (nominal)
The 48h No-Observed Effect Concentration (NOEC): 100 mg/L (nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Acute toxicity of the test item on Daphnia magna was assessed in an Acute immobilisation test, over an exposure period of 48 hours in a static test system (OECD TG 202).
Because no toxic response was observed during the preliminary range-finding test, a Limit Test was carried out using only one test concentration (100 mg/L) and one control group in the definitive test.
The test concentration was analytically determined at the start and at the end of the experiment. Measured concentration was 105.3 mg/L at the start and 102.9 mg/L at the end of the experiment.
As the measured concentration deviated not more than 20 per cent from the nominal, biological results are based on the nominal concentration.
Twenty animals, divided into four groups (glass beaker) of five animals each were used at the test concentration and for the control.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of the test item were as follows:
The 24h and 48h EC50 value: > 100 mg/L (nominal)
The 48h EC100 value: > 100 mg/L (nominal)
The 48h No-Observed Effect Concentration (NOEC): 100 mg/L (nominal)
The 48h Lowest Observed Effect Concentration (LOEC): > 100 mg/L (nominal)
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