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EC number: 695-619-1 | CAS number: 623-72-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021-11-12 ~ 2022-01-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- OECD Guideline for Testing of Chemicals, Test No. 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test (28 Jul, 2011)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl 3-hydroxypropanoate
- Cas Number:
- 623-72-3
- Molecular formula:
- C5H10O3
- IUPAC Name:
- ethyl 3-hydroxypropanoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 1. Solubility test
Solubility test was conducted using the test solution of 100.000 mg/L prepared by stirring (150 ± 30 RPM, 22.5 ± 1.5 ˚C for 0.5, 24, and 48 hours) and ultrasonication (20 kHz, 0.5 hours). The test solution was filtered using a 0.2 μm PVDF syringe filters. Then the 100.000 mg/L nominal concentration test solution was analyzed using the HPLC analyzer.
2. Limit and range finding test (stability test)
Stability test was conducted using the test solution of 10.000 and 100.000 mg/L prepared by stirring (150 ± 30 RPM, 22.5 ± 1.5 ˚C, 0.5 hours). The test solutions were collected at 0 and 72 hr and filtered using 0.2 μm PVDF syringe filters. Then the test solutions were analyzed using the HPLC analyzer.
3. Definitive test
Test solutions of the control and 100.000 mg/L were collected at 0 hour and 72 hours, and filtered using 0.2 μm PVDF syringe filters. Then the test solutions were analyzed using the HPLC analyzer.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 1. Solubility test
Prior to the limit and range finding test, a solubility test was conducted to identify the solubility of the test substance in the dilution water in compliance with ‘Guidance document on aqueous-phase aquatic toxicity testing of difficult test chemicals (OECD Environmental Health and Safety Publications Series on Testing and Assessment No. 23)’.
The test solution of 100.000 mg/L was prepared by adding 0.1025 g of the test substance (purity 97.55 %) to a 1 L volumetric flask and the volume was filled up to the mark with dilution water. Then the test solution was dissolved using a stirrer (150 ± 30 RPM, 22.5 ± 1.5 ˚C for 0.5, 24, and 48 hours) and ultrasonication (20 kHz, 0.5 hours), and filtered with a 0.2 μm PVDF membrane filter (Lot No. 5402236, Hyundai Micro, Korea) and a vacuum pump (GAST, DOA-P704-AC, IDEX Corporation USA).
In results of the solubility test, the solubility of the test substance prepared after 0.5, 24, and 48 hours stirring were 96.682, 96.451, and 95.580 mg/L, respectively, and the solubility by 0.5 hours ultrasonication was 98.398 mg/L. Based on the solubility test, stirring (150 ± 30 RPM, 22.5 ± 1.5 ˚C, 0.5 hours) was selected as the preparation method.
2. Limit and range finding test (stability test)
A stock solution of 100.000 mg/L was prepared by adding 0.1025 g of the test substance to a 1 L volumetric flask and dilution water was added to the mark to make the total volume. Then the test substance was dissolved using a stirrer (150 ± 30 RPM, 22.5 ± 1.5 ℃, 0.5 h) and filtered with a 0.2 μm PVDF membrane filter (Lot No. 5402236, Hyundai Micro, Korea) before use. Limit and range finding tests were conducted at the nominal concentrations of 0.500, 1.000, 10.000, 50.000, and 100.000 mg/L. Each test solution was prepared by diluting the stock solution on the initiation of exposure date.
3. Definitive test
A stock solution of 100.000 mg/L was prepared by adding 0.1025 g of the test substance to a 1 L volumetric flask and dilution water was added to the mark to make the total volume. Then the test substance was dissolved using a stirrer (150 ± 30 RPM, 22.5 ± 1.5 ℃, 0.5 h) and filtered with a 0.2 μm PVDF membrane filter (Lot No. 5402236, Hyundai Micro, Korea) before use. The definitive test was conducted at the nominal concentrations of 100.000 mg/L as a limit test. Each test solution was prepared by diluting the stock solution on the initiation of exposure date.
Test organisms
- Test organisms (species):
- other: Pseudokirchneriella subcapitata
- Details on test organisms:
- # Test species information
- Test species : Pseudokirchneriella subcapitata
- Supplier : Risk Assessment Division, NIER
- Justification of selection : Pseudokirchneriella subcapitata is a suitable plant model for toxicity studies and has been widely used. Abundant data exist for this species and are available for use in interpretation and evaluation for this type of study.
- Selection of the test species : After being examined for morphological alteration (swelling, flocculation, atrophy, decoloration, and rupture), freshwater algae that reached the exponential
growth stage were used for the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Morphological abnormality (swelling, flocculation, atrophy, decoloration, and rupture) of algal cells in each control and test group was checked by microscope and hemocytometer at the end of the test (72 hours).
Test conditions
- Test temperature:
- 22.2-22.3 ˚C
- pH:
- Before exposure (0 hour), and the pH of the control : 8.27 and 7.79
At the end of the test (72 hours), the pH was 7.89 and 7.86 - Nominal and measured concentrations:
- The test solutions of the control and the 100.000 mg/L test group were collected and analyzed at 0 and 72 hours.
The measured concentrations in the nominal concentration (100.000 mg/L) were 100.934 mg/L at 0 hour and 97.066 mg/L at 72 hours. The concentration change of test substance in the nominal concentration (100.000 mg/L) after 72 hours was -3.8 %.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 98.981 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable
- Details on results:
- In results of the acute toxicity test of Ethyl 3-hydroxypropanoate in freshwater algae (Pseudokirchneriella subcapitata), the 72-hour EC50 of yield and average specific growth rate based on the nominal concentration was > 100.000 mg/L, and based on the measured concentration was > 98.981 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In results of the acute toxicity test of Ethyl 3-hydroxypropanoate in freshwater algae (Pseudokirchneriella subcapitata), the 72-hour EC50 of yield and average specific growth rate based on the nominal concentration was > 100.000 mg/L, and based on the measured concentration was > 98.981 mg/L.
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