Diisooctyl succinate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diisooctyl succinate

EC Number:

249-282-9

EC Name:

Diisooctyl succinate

Cas Number:

28880-24-2

Molecular formula:

C20H38O4

IUPAC Name:

bis(6-methylheptyl) succinate

Test material form:

liquid

Test organisms

Test organisms (species):

Anabaena sp.

Details on test organisms:

Organism: The fresh water algal strain “Anabaena sp.” was used for this study. Commercially available media for blue-green algae BG-11 was used at concentration of 1.64 gm in 1 litre demineralized Water.

Study design

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

21–24°C controlled at ± 2 °C

pH:

8.2 ± 1.5

Details on test conditions:

Illumination : 2960- 4440 lux
Growth medium : BG-11 Medium
Apparatus to determine algal biomass : UV-Vis Spectrophotometer
No. of replicates : Five in control and three in test groups

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

6.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the condition of this test, it was concluded that maximum concentration of ‘Dicapryl Succinate’ at 0.1 mg/l elicited no inhibitory effects on the yield and specific growth rate of algae strain ‘Anabaena sp.’. Therefore the effective concentrations (ECs) and no observed effect concentration (NOEC) came out as:
EC10 : 1.3 mg/l
EC20 : 2.7 mg/l
EC50 : 6.7 mg/l
NOEC : 0.1 mg/l
The study was conducted as per the procedure described in the OECD Guideline for the testing of Chemicals, Freshwater Algae and Cyanobacteria, Growth Inhibition Test Guideline No. 201 Adopted on 28th July 2011.

Executive summary:

The study was conducted to determine the growth inhibition effect of ‘Dicapryl Succinate’on algae strain ‘Anabaena sp.’

A range finding study was conducted at four concentrations 1 mg/l, 10 mg/l, 50 mg/l & 100 mg/l of test item. Significant growth inhibitions were found at these concentrations.

Therefore, on the basis of range finding study, main study was conducted using concentration of 0.1, 0.5, 1, 5 and 10 mg/l in BG-11 medium.

The temperature was maintained within the range of 23.6-24.1°C and light intensity within the range of 4252-4265 Lux throughout the experimentation.

Under the condition of this test, it was concluded that ‘Dicapryl Succinate’ elicited no inhibition effects at 0.1 mg/l on the yield and specific growth rate of algae strain ‘Anabaena sp’. Therefore, the Effective concentrations (ECs) and No observed effect concentration (NOEC) came out as:

EC10 : 1.3 mg/l

EC20 : 2.7 mg/l

EC50 : 6.7 mg/l

NOEC : 0.1 mg/l

This study was hence, performed to assess the ‘Freshwater Algae and Cyanobacteria, Growth Inhibition Test By “Dicapryl Succinate”