Methyl 5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04 April 2013 to 26 September 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Fully GLP compliant and in accordance with current test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methylfuranoside

IUPAC Name:

Methylfuranoside

Constituent 2

Reference substance name:

Methyl 5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside

EC Number:

245-485-1

EC Name:

Methyl 5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside

Cas Number:

23202-81-5

Molecular formula:

C9H16O4

IUPAC Name:

methyl 5-deoxy-2,3-O-isopropylidene-beta-D-ribofuranoside

Test material form:

other: Pale yellow liquid

Details on test material:

Name: Methylfuranoside
CAS number: 23202-81-5
Batch number: 201111082008
Quantity received: 1.5 kg
Purity: 99.7%
Expiry date: November 2013
Date of receipt: Not reported
Storage details: stored in a sealed container, at room temperature (15 to 30°C) in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At 0 and 48 hours, duplicate ca 100 mL samples of freshly prepared test media were taken from the control and 125 mg/L test media for chemical analysis.
At 24 and 72 hours, duplicate samples (ca 100 mL) of the control and 125 mg/L test concentration were taken for chemical analysis from the old media.

Test solutions

Vehicle:

yes

Details on test solutions:

The dilution water used for conducting the definitive test was de-chlorinated mains water that had been passed through particulate and activated carbon filters and treated with ozone (O3) to remove residual algal cells and fungal spores for improved water quality.
The initial range-finding test was conducted at nominal test substance concentrations of 1.25, 12.5 and 125 mg/L, under semi-static test conditions.
Based on the results of the range-finding test, a definitive test was conducted at a single nominal test substance concentration of 125 mg/L.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish (Danio rerio)
- Source: University College, London (UCL), United Kingdom
- Age at study initiation (mean and range, SD): Not reported
- Length at study initiation (length definition, mean, range and SD): Mean - 2.95 cm, 2.7 - 3.1 cm
- Weight at study initiation (mean and range, SD): Mean 0.48 g, 0.29 - 0.60 g
- Feeding during test: Not fed during the test
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable

ACCLIMATION
- Acclimation period: 12 days prior to start of test
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Not reported
- Feeding frequency: Not reported
- Health during acclimation (any mortality observed): Healthy

Study design

Test type:

semi-static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness as CaCO3 (mg/L): 0 hour - 65 mg/L, 24 hours (new) - 66 mg/L, 48 hours (new) - 49 mg/L, 72 hours (new) - 47 mg/L

Test temperature:

Control: 0 hour - 23.6°C, 24 hours (old) - 23.4°C, 24 hours (new) - 23.4°C, 48 hours (old) - 23.7°C, 48 hours (new) - 23.2°C, 72 hours (old) - 23.4°C, 72 hours (new) - 23.3°C, 96 hours - 24.0°C
125 mg/L test substance: 0 hour - 23.2°C, 24 hours (old) - 23.5°C, 24 hours (new) - 23.3°C, 48 hours (old) - 23.5°C, 48 hours (new) - 23.3°C, 72 hours (old) - 23.5°C, 72 hours (new) - 23.2°C, 96 hours - 24.1°C

pH:

Control: 0 hour - 7.03, 24 hours (old) - 7.28, 24 hours (new) - 7.27, 48 hour (old) - 7.08, 48 hours (new) - 7.06, 72 hours (old) - 7.34, 72 hours (new) - 7.19, 96 hours - 7.27
125 mg/L test substance: 0 hour - 7.04, 24 hours (old) - 7.30, 24 hours (new) - 7.14, 48 hours (old) - 7.11, 48 hours (new) - 7.05, 72 hours (old) - 7.37, 72 hours (new) - 7.19, 96 hours - 7.33

Dissolved oxygen:

Dissolved oxygen % ASV (mg/L)
Control: 0 hour - 101 (8.64), 24 hours (old) - 99 (8.27), 24 hours (new) - 110 (9.28), 48 hours (old) - 95 (7.82), 48 hours (new) - 100 (8.26), 72 hours (old) - 95 (8.10), 72 hours (new) - 106 (8.78), 96 hours - 95 (8.20)
125 mg/L test substance: 0 hour - 101 (8.61), 24 hours (old) - 97 (8.12), 24 hours (new) - 109 (9.14), 48 hours (old) - 95 (7.84), 48 hours (new) - 100 (8.24), 72 hours (old) - 107 (8.79), 72 hours (new) - 107 (8.79), 96 hours - 95 (8.10)

Salinity:

Not reported

Nominal and measured concentrations:

Nominal concentration - 125 mg/L
0 hours (new media) - 89.79 mg/L (72% nominal)
24 hours (old meadia) - 123.18 mg/L ( 99% nominal)
48 hours (new media) - 122.82 mg/L (98% nominal)
72 hours (old media) - 126.09 mg/L (101% nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 2.5 L constructed glass aquaria containing 10 L of the appropriate test media, fitted with clear plastic lids
- Material, size, headspace, fill volume: 7 L of appropriate test media
- Aeration: Not applicable
- Renewal rate of test solution (frequency/flow rate): 24 hourly
- No. of organisms per vessel: Three
- No. of vessels per concentration (replicates): Single
- No. of vessels per control: Single
- Biomass loading rate: 0.69 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Treated mains water, in laboratory
- Intervals of water quality measurement: At the start of the test and every 24 hours

OTHER TEST CONDITIONS
- Adjustment of pH: Not applicable
- Photoperiod: Not reported
- Light intensity: Not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : All fish in each test vessel were observed at ca 4.5 hours and then at 24-hour intervals (24, 48, 72 and 96 hours) throughout the duration of the test. The number of dead fish and those fish exhibiting toxic symptoms or modified behaviour were recorded, if applicable.

TEST CONCENTRATIONS
- Range finding study: The initial range-finding test was conducted at nominal test substance concentrations of 1.25, 12.5 and 125 mg/L, under semi-static test conditions. A control group containing treated mains water only was also included.
- Results used to determine the conditions for the definitive study: Based on the results of the range-finding test, a definitive test was conducted at a single nominal test substance concentration of 125 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

> 115 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

other: LC20

Effect conc.:

> 115 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 115 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 115 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Chemical analysis
The limit of detection (LOD) for Methylfuranoside in treated mains water using this method was 0.043 mg/L.
Analysis of the fresh media at 0 and 48 hours showed measured concentrations of 72% and 98% of nominal, respectively. Analysis of the corresponding old media at 24 and 72 hours showed measured concentrations of 99% and 101% of nominal, respectively. The mean measured concentration was calculated to be 115 mg/L (equivalent to 92% of nominal value). The results have therefore been based on mean measured test concentrations.
Toxicity
The EC10, EC20 and EC50 values were determined to be greater than 115 mg/L.
Based on mean measured concentrations, the 96-hour NOEC was considered to be 115 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour acute toxicity of Methylfuranoside to the freshwater fish species Danio rerio was determined under semi-static test conditions in accordance with the requirements of OECD Chemicals Testing Guideline No. 203 Fish, Acute Toxicity Test (adopted 17 July 1992).
All validity criteria were satisfied, therefore the test was considered valid.
The 96-hour LC50 value was considered to be greater than 115 mg/L, based on mean measured test concentrations. The NOEC was considered to be 115 mg/L.
It is concluded that Methylfuranoside was considered non toxic to Danio rerio at nominal concentrations less than 125 mg/L under the conditions of this test.

Executive summary:

The objective of the study was to determine the 96-hour acute toxicity of Methylfuranoside to Danio rerio. The study was conducted in accordance with the requirements of OECD Chemicals Testing Guideline No.203 Fish, Acute Toxicity Test (adopted 17 July 1992).

Based on the results of a range-finding test, which are not fully reported, the definitive test was conducted at a single nominal test concentration of 125 mg/L.

The study was conducted using a semi-static test design with daily renewal of test media during the test.

Analysis of the fresh media at 0 and 48 hours showed measured concentrations of 72% and 98% of nominal, respectively. Analysis of the corresponding old media at 24 and 72 hours showed measured concentrations of 99% and 101% of nominal, respectively. The mean measured concentration was calculated to be 115 mg/L (equivalent to 92% of nominal value). The results have therefore been based on mean measured test concentrations.

[1] LC _{10, 20} and ₅₀ values and the corresponding [2] NOEC values are presented in the following table. Values are presented in terms of nominal concentrations.

Parameter		Mean Measured Concentrations (mg/L)
		24 hours	48 hours	72 hours	96 hours
LC10		>115	>115	>115	>115
LC20		>115	>115	>115	>115
LC50		>115	>115	>115	>115
NOEC		115	115	115	115

Based on mean measured test concentrations, the EC₁₀, EC₂₀ and EC₅₀ values were determined to be greater than 115 mg/L.

Based on mean measured test concentrations, the 96-hour NOEC was considered to be 115 mg/L.

The validity criteria for control mortality and dissolved oxygen (>60% ASV) were both satisfied. The test was therefore considered to be valid.

It is concluded thatMethylfuranoside was considerednon toxic to Danio rerio at nominal concentrations less than 125 mg/L under the conditions of this test.

 

[1] Lethal concentration resulting in 10, 20 and 50% mortality

[2] No Observed Effect Concentration