(1Z)-1-Chloro-2,3,3-trifluoroprop-1-ene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16/12/2016 - 17/02/2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(1Z)-1-chloro-2,3,3-trifluoroprop-1-ene

EC Number:

824-458-3

Cas Number:

1263679-68-0

Molecular formula:

C3H2ClF3

IUPAC Name:

(1Z)-1-chloro-2,3,3-trifluoroprop-1-ene

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Strain: ATCC22662
Source: American Type Culture Collection
Date of receipt: June 20, 1996
Maintenance of obtained algae: Algae have been maintained by period subculture using Gorham medium. Algae are kept under sterile condition. Sterile test has been performed periodically, every six months, to confirm that the algae are not contaminated.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

21.4 - 21.7 °C

pH:

7.8 - 10.3

Details on test conditions:

Test vessels and other apparatus were sterilized as necessary. Algae were introduced into culture medium by an aseptic technique.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 82.5 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

13.4 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

growth rate

Details on results:

In all test groups, the color of the test cultures (as observed with the naked eye) showed a tendency to get more greenish during the exposure period.
By microscopic observation at the end of exposure, in all concentration groups neither unusual cell shape of algae (contraction, expansion, damaged cell etc.) nor agglutination was observed, and the algae looked normal compared with those in the control group.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A growth inhibition test of the test substance with Pseudokirchneriella subcapitata was conducted in order to determine the 50% growth inhibition concentration (EC50) and the no observed effect concentration (NOEC) after 72-hour exposure.

The results were calculated from the time weighted mean of measured concentrations.
50% growth inhibition concentration, ErC50 (0-72h): >82.5 mg/L
No observed effect concentration, NOECr (0-72h): 13.4 mg/L

Executive summary:

A growth inhibition test of the test substance with Pseudokirchneriella subcapitata was conducted in order to determine the 50% growth inhibition concentration (EC50) and the no observed effect concentration (NOEC) after 72-hour exposure.

The results were calculated from the time weighted mean of measured concentrations.

50% growth inhibition concentration, ErC50 (0-72h): >82.5 mg/L

No observed effect concentration, NOECr (0-72h): 13.4 mg/L