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EC number: 270-220-1 | CAS number: 68413-48-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 May 2006 to 30 May 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Sample storage: room temperature
- Analytical monitoring:
- no
- Remarks:
- The investigation was carried out without accompanying analysis to check nominal concentrations.
- Details on sampling:
- Sample storage: room temperature
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism: Daphnia magna, var. Straus,
Source: Dr. Knie LWA
Breeding: kept in drinking water at room temperature (20 +/- 1 °C) under diffuse lighting (16 hours a day); Young animals are separated daily, so that tested daphnia are not older than 24 hours and not younger than 6 hours
Feed: Daily with cells of Desmodesmus subspicatus - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1 °C
- pH:
- 7.78 - 8.55
- Dissolved oxygen:
- 6.8 - 8.9 mg O2/L
- Nominal and measured concentrations:
- 100 mg/L (nominal)
- Details on test conditions:
- Static test over 48 hours, 4 parallel assays with each 5 animals per test item concentration
The daphnia were exposed to various test substance concentrations over 48 hours. Since the substance is difficult to dissolve in water, 100 mg of the substance were suspended in daphnia-dilution-water and shaken for 24 hours on the shaker at about 130 rpm before starting the test. It is presumed that during this time there is an equilibrium between water-soluble and water-insoluble part of the substance. After this incubation, the suspension was filtered through a glass fiber filter. The clear filtrate (eluate) was used directly without further dilution in the daphnia test. After 24 and 48 hours, the number of daphnia unable to swim was determined. pH value, water temperature and dissolved oxygen content were measured at the beginning and the end of the test. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The investigation was carried out without accompanying analysis to check nominal concentrations.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- No effects on mobilisation of daphnia were observed, resulting in an EC50 of greater than 100 mg/L under test conditions according to OECD Guideline 202.
- Executive summary:
Daphnia toxicity of Butanedioic acid, [[bis(2-ethylhexyl)oxy]phosphinothioyl]thio]-, dibutylester was analyzed in a static limit test over 48 hours according to OECD guideline 202 (immobilisation test).
Since the substance is difficult to dissolve in water, 100 mg of the substance was suspended in daphnia-dilution-water and shaken for 24 hours on the shaker at about 130 rpm before starting the test. It was presumed that during this time there is an equilibrium between water-soluble and water-insoluble part of the substance. After this incubation, the suspension was filtered through a glass fiber filter. The clear filtrate (eluate) was used directly without further dilution in the daphnia test. After 24 and 48 hours, the number of daphnia unable to swim was determined. pH value, water temperature and dissolved oxygen content were measured at the beginning and and the end of the test. No effects on mobilisation of daphnia were observed, resulting in an EC50 of greater than 100 mg/L under test conditions according to OECD Guideline 202.
Reference
The number of immobilized animals per parallel assay were determined after 24 and 48 hours.
Description of key information
In an OECD 202 study, the acute toxicity of the test item to the freshwater invertebrate Daphnia magna was investigated under static conditions and the 48-hour EC50 value was determined to be greater than 100 mg/L loading rate WAF (SGS INSTITUT FRESENIUS GmbH, 2006).
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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