Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 930-930-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11/04/2018 - 18/05/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Remarks:
- test item directly weighed into the test vessels.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 10 g/L. Therefore, weighed amounts were added to the test bottles containing Milli-RO water. The test item – Milli-RO water mixtures were magnetically stirred for a short period and subsequently, synthetic medium, sludge and Milli - RO water were added resulting in the required concentrations. Optimal contact between the test item and test organisms was ensured by applying continuous aeration and stirring.
- Eluate: Milli-RO water
- Controls: blank control, abiotic control, positive control (reference substance).
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Municipal sewage treatment plant: 'Waterschap Aa en Maas's -Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge, as used for the test. The batch of sludge was used one day after collection; therefore 50 mL of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Pretreatment: The initial pH of the sludge was 5.7 on the day of testing. The sludge was buffered to a pH of 7.0 using a 70 g/L sodium bicarbonate solution. Before the sludge was used, the respiration rate of the sludge was determined to verify the quality of the sludge. The mean respiration rate was 38 mg O2/g.h and therefore, within the validity criterion. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 17 - 22ºC (slightly outside the range prescribed by the study plan for 1.5h, the deviation is considered to have no effect on the outcome of the test).
- pH:
- The pH in all test vessels, before addition of sludge, was between 7.6 and 9.5. After the 3 hour exposure period the pH was between 6.6 and 10.3.
- Dissolved oxygen:
- 60-70% saturation (> 5 mg/L at 20ºC)
- Nominal and measured concentrations:
- - range-finding test: 0(control), 10, 100, 1000 mg/L test item (nominal).
- main test: 0 (control), 10, 32, 100, 320, 1000 mg/L test item (nominal). - Details on test conditions:
- TEST SYSTEM
- Test vessel: all glass open bottles
- Type (delete if not applicable): open
- Aeration: yes. The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (> 5 mg/L) and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- No. of vessels per abiotic control (replicates): 5
- Sludge concentration (weight of dry solids per volume): 3.0 g/L
- Weight of dry solids per volume of reaction mixture per unit of time: 1.5 g/L
- Nutrients provided for bacteria: 50 mL of synthetic medium per litre of activated sludge.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: RO water (tap water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
OTHER TEST CONDITIONS
- Adjustment of pH: no.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Oxygen consumption was measured over a period of 10 min after 3 h incubation of the test vessels. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. The pH was determined in the remaining part of the reaction mixture. This procedure was repeated for all test/reference item concentrations and controls. The medium temperature was recorded continuously in temperature control vessels.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study : yes. The final test was performed based on the result of a preceding combined limit/range-finding test.
- Test concentrations: 10, 100 and 1000 mg/L in the range finding test; 10, 32, 100, 320 and 1000 mg/L in the final test.
- Results used to determine the conditions for the definitive study: The combined limit/range-finding showed no inhibition, 24% and 91% inhibition of the respiration rate at a concentration of 10, 100 and 1000 mg/L, respectively. Therefore, the expected EC50 was between 100 and 1000 mg/L. Also, there was a significant oxygen production from the abiotic processes. The oxygen production was 17% of the mean respiration rate of the control. Therefore it was decided to perform the final tests with an addition of an abiotic control of all concentrations. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (Sigma-Aldrich Chemie GmbH, Steinheim, Germany, Lot no. MKBZ0947V)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 194 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: (95% CL: 165-229 mg/L)
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 63 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: (95% CL: 42-82 mg/L)
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 93 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: (95% CL: 69-114 mg/L)
- Details on results:
- - No statistically significant inhibition of the respiration rate of the sludge was recorded at 32 mg test item. At higher concentrations the inhibitory effect of the test item on aerobic waste water (activated sludge) bacteria increased with increasing concentration, ranging from 21% inhibition at 100 mg/L to 97% at 1000 mg/L.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no.
- Effect concentrations exceeding solubility of substance in test medium: no.
- Adsorption (e.g. of test material to the walls of the test container): no.
- Blank controls oxygen uptake rate: The mean control oxygen uptake rate exceeded 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour (29 mg oxygen per one gram of activated sludge in the final test).
- Coefficient of variation of oxygen uptake rate in control replicates: The coefficient of variation of oxygen uptake in control replicates did not exceed 30% at the end of the definitive test (18%). - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/L for total respiration (3.2 mg/L in the final test). - Reported statistics and error estimates:
- Calculation of the ECx value was based on probit analysis using linear maximum likelihood regression, with the percentages of respiration inhibition versus the logarithms of the corresponding concentrations of the test item. Calculations were performed with ToxRat Professional v. 3.2.1 (ToxRat Solutions® GmbH, Germany).
- Validity criteria fulfilled:
- yes
- Remarks:
- The mean control oxygen uptake rate was 29 mg O2 per gram of activated sludge in the final test; the coefficient of variation of oxygen uptake in control replicates was 18%; the EC50 of the reference substance was within the acceptable range.
- Conclusions:
- The 3h-EC50 of the test item on aerobic waste water (activated sludge) bacteria was 194 mg/L, the NOEC was 32 mg/L.
- Executive summary:
A study on the activated sludge respiration inhibition of the test item was carried out according to OECD 209 (GLP study). The final test was performed based on the result of a preceding combined limit/range-finding test. The respiration rates of samples of activated sludge from a sewage treatment plant treating predominantly domestic sewage (1.5 g/L suspended solids) and fed with synthetic sewage was measured in an enclosed cell containing an oxygen electrode after a contact time (incubation) of 3 hours. Five concentrations were tested, ranging from 10 to 1000 mg/L and increasing with a factor 3.2. Five replicates per concentration and six replicates for an untreated control group were tested. An abiotic control was performed for all test groups to correct for the oxygen production of the test item (five replicates per group). Since there was an oxygen production observed in the abiotic control with more than 5% in the highest concentration, the respiration rates of the test item are corrected with the respiration rate of the abiotic control from the corresponding concentration. The study met the acceptability criteria. No statistically significant inhibition of the respiration rate of the sludge was recorded at 32 mg/L test item. At higher concentrations, the inhibitory effect of the test item on aerobic waste water (activated sludge) bacteria increased with increasing concentration, ranging from 21% inhibition at 100 mg/L to 97% at 1000 mg/L. The 3h-EC50 of the test item on aerobic waste water (activated sludge) bacteria was 194 mg/L (95% CL: 165 - 229 mg/L), the EC20 was 93 mg/L (69 - 114 mg/L) and the EC10 was 63 mg/L (42 - 82 mg/L). The test itemwas not toxic to waste water (activated sludge) bacteria at a concentration of 32 mg/L (NOEC).
Reference
Table 1. Effect parameters for the inhibition of the respiration rate.
Parameter |
Test item concentration (mg/L) |
NOEC |
32 |
EC10 |
63 (42 – 82) |
EC20 |
93 (69 – 114 ) |
EC50 |
194 (165 – 229) |
( ) Between brackets the 95% confidence intervals are given.
Table 2. Combined limit/range-finding Test – Weighed amounts of test item.
Treatment |
Concentration (mg/L) |
Weighed amount (mg) of test item |
||
Replicate A |
Replicate B |
Replicate C |
||
T1 |
10 |
5.06 |
- |
- |
T2 |
100 |
49.75 |
- |
- |
T3 |
1000 |
499.92 |
500.02 |
500.01 |
TA (abiotic) |
1000 |
499.96 |
- |
- |
- : Not applicable
Table 3. Final Test – Weighed amounts of test item.
Treatment |
Concentration (mg/L) |
Weighed amount (mg) of test item |
||||
Replicate A |
Replicate B |
Replicate C |
Replicate D |
Replicate E |
||
T1 |
10 |
4.99 |
5.00 |
5.02 |
5.00 |
5.03 |
T2 |
32 |
16.02 |
15.96 |
15.98 |
15.99 |
15.96 |
T3 |
100 |
50.01 |
49.93 |
49.96 |
49.96 |
49.99 |
T4 |
320 |
160.04 |
160.02 |
159.96 |
159.98 |
159.98 |
T5 |
1000 |
499.77 |
499.89 |
499.98 |
499.90 |
499.98 |
TA1 |
10 |
4.96 |
4.98 |
4.97 |
5.00 |
5.02 |
TA2 |
32 |
15.96 |
15.96 |
15.97 |
16.04 |
15.97 |
TA3 |
100 |
49.98 |
50.02 |
49.96 |
49.97 |
49.96 |
TA4 |
320 |
159.98 |
160.04 |
159.99 |
159.98 |
159.96 |
TA5 |
1000 |
499.99 |
499.69 |
499.91 |
499.93 |
499.98 |
Table 4. Combined Limit/Range-Finding Test – Experimental Set-Up.
Type of bottle |
Test/reference item |
Synthetic medium |
Made up with Milli-RO water |
Sludge |
Total volume mixture 250 mL |
||||
Control (C) |
no |
16 mL |
yes |
250 mL |
Reference item concentrations (R ) |
yes |
16 mL |
yes |
250 mL |
Test item concentrations (T) |
yes |
16 mL |
yes |
250 mL |
Abiotic control of test item, Highest concentration (TA) |
yes |
16 mL |
yes |
No sludge, made up to 500 mL with Milli-RO water |
Table 5. Final – Experimental Set-Up.
Type of bottle |
Test/reference item |
Synthetic medium |
Made up with Milli-RO water |
Sludge |
Total volume mixture 250 mL |
||||
Control (C) |
no |
16 mL |
yes |
250 mL |
Reference item concentrations (R) |
yes |
16 mL |
yes |
250 mL |
Test item1 concentrations (T) |
yes |
16 mL |
yes |
250 mL |
Abiotic control, test item1 concentrations (TA) |
yes |
16 mL |
yes |
No sludge, made up to 500 mL with Milli-RO water |
Table 6. Final Test – Overview of the oxygen production of abiotic control.
Treatment |
Concentration (mg/L) |
Mean respiration rate |
% uptake, corresponding with the mean control (mean value) |
|
(mg O2/L h) |
(mg O2/g h)¹ |
|||
TA1 |
10 |
-0.54 |
-0.36 |
1.26 |
TA2 |
32 |
-0.71 |
-0.47 |
1.66 |
TA3 |
100 |
-1.56 |
-1.04 |
3.66 |
TA4 |
320 |
-2.22 |
-1.48 |
5.19 |
TA5 |
1000 |
-3.43 |
-2.29 |
8.03 |
¹) The amount of suspended solids in the final test mixture was 1.5 g/L
Table 7. Final Test – Overview of the Results, corrected for oxygen production in the abiotic control.
Treatment |
Concentration (mg/L) |
Mean respiration rate |
% Inhibition of the respiration rate (mean value) |
|
(mg O2/L h) |
(mg O2/g h)¹ |
|||
Control |
|
42.77 |
28.51 |
|
T1 |
10 |
46.61 |
31.07 |
-8.97 |
T2 |
32 |
44.45 |
29.63 |
-3.91 |
T3 |
100 |
33.88 |
22.59 |
20.78* |
T4 |
320 |
12.11 |
8.07 |
71.68* |
T5 |
1000 |
1.16 |
0.77 |
97.29* |
¹) The amount of suspended solids in the final test mixture was 1.5 g/L.
* Statistically significantly different compared to control.
Table 8. Combined Limit/Range-Finding Test – Respiration Rate/Inhibition, pH Values.
Replicate |
Concentration (mg/L) |
pH |
Respiration rate |
% Inhibition respiration rate (mean value) |
||
Start |
End |
(mg O2/L.h) |
(mg O2/g.h)* |
|||
C 1 |
0 |
7.4 |
8.3 |
42.09 |
28.06 |
|
C 2 |
0 |
7.4 |
8.3 |
43.20 |
28.80 |
|
C 3 |
0 |
7.4 |
8.2 |
51.88 |
34.59 |
|
C 4 |
0 |
7.4 |
8.3 |
47.27 |
31.51 |
|
C 5 |
0 |
7.4 |
8.2 |
48.43 |
32.29 |
|
C 6 |
0 |
7.4 |
8.2 |
48.37 |
32.25 |
|
C Mean |
|
|
|
46.87 |
31.25(RC) |
|
SD |
|
|
|
3.64 |
2.43 |
|
CV (%) |
|
|
|
8 |
8 |
|
|
|
|
|
|
|
|
R 1 |
1.0 |
7.4 |
8.2 |
54.56 |
36.37 |
-16.40 |
R 2 |
3.2 |
7.4 |
8.4 |
31.16 |
20.77 |
33.52 |
R 3 |
10 |
7.4 |
8.4 |
20.79 |
13.86 |
55.65 |
R 4 |
32 |
7.4 |
8.5 |
8.23 |
5.49 |
82.44 |
|
|
|
|
|
|
|
T 1 |
10 |
7.8 |
8.3 |
48.00 |
32.00 |
-2.40 |
T 2 |
100 |
9.0 |
8.5 |
35.41 |
23.61 |
24.46 |
T 3a |
1000 |
10.0 |
9.4 |
3.30 |
2.20 |
92.96 |
T 3b |
1000 |
10.0 |
9.5 |
2.90 |
1.93 |
93.81 |
T 3c |
1000 |
9.5 |
9.4 |
6.99 |
4.66 |
85.09 |
T3 Mean |
|
|
4.40 |
2.93 (RT)* |
90.62 (IT) |
|
|
|
|
|
|
|
|
TA |
1000 |
9.4 |
9.9 |
-8.07# |
-5.38 |
117.22 |
C: Control; R: Reference item, 3,5-dichlorophenol; T: Test item, Reaction mass of calcium dihydroxide and calcium peroxide, EC 930-930-0; TA: Abiotic control of test item; SD: Standard deviation; CV: Coefficient of variation; RT: Total respiration with test item; RC: Total respiration in the control; IT: % inhibition of total respiration relative to RC; *The amount of suspended solids in the final test mixture was 1.5 g/L. # Oxygen production, therefore expressed as negative value.
Table 9. Final Test –Respiration Rate/Inhibition, pH Values.
|
Concentration (mg/L) |
pH |
Respiration rate |
% Inhibition respiration rate (mean value) |
||
Start |
End |
(mg O2/L.h) |
(mg O2/g.h)* |
|||
C 1 |
0 |
7.6 |
7.4 |
49.61 |
33.07 |
|
C 2 |
0 |
7.6 |
7.2 |
33.18 |
22.12 |
|
C 3 |
0 |
7.6 |
6.7 |
52.85 |
35.23 |
|
C 4 |
0 |
7.6 |
6.6 |
43.77 |
29.18 |
|
C 5 |
0 |
7.6 |
6.7 |
34.65 |
23.10 |
|
C 6 |
0 |
7.6 |
6.7 |
42.57 |
28.38 |
|
C Mean |
|
|
|
42.77 |
28.51 (RC) |
|
SD |
|
|
|
7.84 |
5.23 |
|
CV (%) |
|
|
|
18 |
18 |
|
|
|
|
|
|
|
|
R 1 |
1.0 |
7.6 |
7.7 |
35.53 |
23.69 |
16.93 |
R 2 |
3.2 |
7.6 |
8.0 |
19.45 |
12.97 |
54.53 |
R 3 |
10 |
7.6 |
7.9 |
12.40 |
8.27 |
71.01 |
R 4 |
32 |
7.6 |
7.9 |
5.01 |
3.34 |
88.29 |
|
|
|
|
|
|
|
T 1a |
10 |
8.0 |
7.3 |
55.02 |
36.68 |
-28.64 |
T 1b |
10 |
8.0 |
7.3 |
44.06 |
29.37 |
-3.01 |
T 1c |
10 |
8.0 |
7.3 |
40.73 |
27.15 |
4.77 |
T 1d |
10 |
8.0 |
7.2 |
46.69 |
31.13 |
-9.16 |
T 1e |
10 |
8.0 |
7.2 |
43.86 |
29.24 |
-2.54 |
T 1 Mean |
|
|
|
46.07 |
30.71 (RT) |
-7.72 (IT) |
|
|
|
|
|
|
|
T 2a |
32 |
8.6 |
7.5 |
52.89 |
35.26 |
-23.66 |
T 2b |
32 |
8.6 |
7.4 |
45.40 |
30.27 |
-6.15 |
T 2c |
32 |
8.6 |
7.4 |
41.49 |
27.66 |
3.00 |
T 2d |
32 |
8.6 |
7.6 |
34.14 |
22.76 |
20.18 |
T 2e |
32 |
8.6 |
7.3 |
44.76 |
29.84 |
-4.65 |
T 2 Mean |
|
|
|
43.74 |
29.16 (RT) |
-2.25 (IT) |
|
|
|
|
|
||
T 3a |
100 |
8.8 |
8.0 |
43.50 |
29.00 |
-1.70 |
T 3b |
100 |
8.9 |
8.1 |
32.28 |
21.52 |
24.53 |
T 3c |
100 |
8.9 |
8.1 |
26.71 |
17.81 |
37.55 |
T 3d |
100 |
8.9 |
8.1 |
32.09 |
21.39 |
24.97 |
T 3e |
100 |
8.9 |
8.1 |
27.02 |
18.01 |
36.83 |
T 3 Mean |
|
|
|
32.32 |
21.55 (RT) |
24.44 (IT) |
|
|
|
|
|
|
|
T 4a |
320 |
9.0 |
9.0 |
11.77 |
7.85 |
72.48 |
T 4b |
320 |
9.0 |
9.2 |
9.30 |
6.20 |
78.26 |
T 4c |
320 |
9.0 |
9.0 |
8.99 |
5.99 |
78.98 |
T 4d |
320 |
9.0 |
9.1 |
9.53 |
6.35 |
77.72 |
T 4e |
320 |
9.0 |
9.2 |
9.87 |
6.58 |
76.92 |
T 4 Mean |
|
|
|
9.89 |
6.59 (RT) |
76.87 (IT) |
|
|
|
|
|
|
|
T 5a |
1000 |
9.4 |
9.9 |
-2.88 |
-1.92 |
106.73 |
T 5b |
1000 |
9.4 |
9.8 |
-1.20 |
-0.80 |
102.81 |
T 5c |
1000 |
9.4 |
9.9 |
-1.87 |
-1.25 |
104.37 |
T 5d |
1000 |
9.4 |
9.8 |
-2.60 |
-1.73 |
106.08 |
T 5e |
1000 |
9.4 |
9.9 |
-2.82 |
-1.88 |
106.59 |
T 5 Mean |
|
|
|
-2.27# |
-1.52 (RT)# |
105.32 (IT) |
C: Control; SD: Standard deviation; CV: Coefficient of variation; R: Reference item, 3,5-dichlorophenol; T: Test item, Reaction mass of calcium dihydroxide and calcium peroxide, EC 930-930-0; *: The amount of suspended solids in the final test mixture was 1.5 g/L; RC: Total respiration in the control; RT: Total respiration with test item; IT: % inhibition of total respiration relative to RC; # : Oxygen production, therefore expressed as negative value.
Table 10. Final Test – Respiration Rate/Inhibition abiotic control, pH Values.
|
Concentration (mg/L) |
pH |
Respiration rate# |
% Inhibition respiration rate (mean value) |
||
Start |
End |
(mg O2/L.h) |
(mg O2/g.h)* |
|||
TA 1a |
10 |
8.0 |
7.8 |
-0.38 |
-0.25 |
100.89 |
TA 1b |
10 |
8.0 |
7.7 |
-0.06 |
-0.04 |
100.14 |
TA 1c |
10 |
8.0 |
7.8 |
-0.45 |
-0.30 |
101.05 |
TA 1d |
10 |
8.0 |
7.7 |
-0.74 |
-0.49 |
101.73 |
TA 1e |
10 |
8.0 |
7.7 |
-1.06 |
-0.71 |
102.48 |
TA 1 Mean |
|
|
|
-0.54 |
-0.36 (RTA) |
101.26 (ITA) |
|
|
|
|
|
|
|
TA 2a |
32 |
8.6 |
8.0 |
-0.62 |
-0.41 |
101.45 |
TA 2b |
32 |
8.6 |
8.0 |
-0.41 |
-0.27 |
100.96 |
TA 2c |
32 |
8.6 |
8.0 |
-0.86 |
-0.57 |
102.01 |
TA 2d |
32 |
8.6 |
8.0 |
-1.06 |
-0.71 |
102.48 |
TA 2e |
32 |
8.6 |
8.0 |
-0.60 |
-0.40 |
101.40 |
TA 2 Mean |
|
|
|
-0.71 |
-0.47 (RTA) |
101.66 (ITA) |
|
|
|
|
|
|
|
TA 3a |
100 |
8.9 |
8.6 |
-2.21 |
-1.47 |
105.17 |
TA 3b |
100 |
8.9 |
8.5 |
-0.66 |
-0.44 |
101.54 |
TA 3c |
100 |
8.9 |
8.6 |
-1.88 |
-1.25 |
104.40 |
TA 3d |
100 |
8.9 |
8.8 |
-1.02 |
-0.68 |
102.38 |
TA 3e |
100 |
8.9 |
8.5 |
-2.05 |
-1.37 |
104.79 |
TA 3 Mean |
|
|
|
-1.56 |
-1.04 (RTA) |
103.66 (ITA) |
|
|
|
|
|
|
|
TA 4a |
320 |
9.1 |
9.6 |
-2.94 |
-1.96 |
106.87 |
TA 4b |
320 |
9.1 |
9.7 |
-2.78 |
-1.85 |
106.50 |
TA 4c |
320 |
9.1 |
9.6 |
-2.47 |
-1.65 |
105.77 |
TA 4d |
320 |
9.1 |
9.8 |
-0.86 |
-0.57 |
102.01 |
TA 4e |
320 |
9.1 |
9.8 |
-2.05 |
-1.37 |
104.79 |
TA 4 Mean |
|
|
|
-2.22 |
-1.48 (RTA) |
105.19 (ITA) |
|
|
|
|
|
|
|
TA 5a |
1000 |
9.4 |
10.3 |
-3.45 |
-2.30 |
108.07 |
TA 5b |
1000 |
9.5 |
10.0 |
-4.39 |
-2.93 |
110.26 |
TA 5c |
1000 |
9.5 |
10.1 |
-2.80 |
-1.87 |
106.55 |
TA 5d |
1000 |
9.5 |
10.1 |
-2.71 |
-1.81 |
106.34 |
TA 5e |
1000 |
9.5 |
9.9 |
-3.82 |
-2.55 |
108.93 |
TA.5 Mean |
|
|
|
-3.43 |
-2.29 (RTA) |
108.03 (ITA) |
TA: Abiotic control of test item; *: The amount of suspended solids in the final test mixture was 1.5 g/L; RTA: Total respiration of the abiotic control with test item; ITA: % inhibition of total respiration of the abiotic control, relative to RC; #: Oxygen production, therefore expressed as negative value.
Table 11. Final Test – Respiration Rate/Inhibition with correction for the abiotic control.
|
Concentration (mg/L) |
Respiration rate |
% Inhibition respiration rate (mean value) |
|
(mg O2/L.h) |
(mg O2/g.h) # |
|||
T1a |
10 |
55.56 |
37.04 |
-29.89 |
T1b |
10 |
44.60 |
29.73 |
-4.27 |
T1c |
10 |
41.27 |
27.51 |
3.52 |
T1d |
10 |
47.23 |
31.49 |
-10.42 |
T1e |
10 |
44.40 |
29.60 |
-3.80 |
T 1 Mean |
|
46.61 |
31.07 (RT) |
-8.97 (IT) |
|
|
|
|
|
T2a |
32 |
53.60 |
35.73 |
-25.32 |
T2b |
32 |
46.11 |
30.74 |
-7.81 |
T2c |
32 |
42.20 |
28.13 |
1.34 |
T2d |
32 |
34.85 |
23.23 |
18.52 |
T2e |
32 |
45.47 |
30.31 |
-6.31 |
T 2 Mean |
|
44.45 |
29.63 (RT) |
-3.91 (IT) |
|
|
|
|
|
T3a |
100 |
45.06 |
30.04 |
-5.36 |
T3b |
100 |
33.84 |
22.56 |
20.87 |
T3c |
100 |
28.27 |
18.85 |
33.90 |
T3d |
100 |
33.65 |
22.44 |
21.32 |
T3e |
100 |
28.58 |
19.06 |
33.17 |
T3 Mean |
|
33.88 |
22.59 (RT)* |
20.78 (IT)* |
|
|
|
|
|
T4a |
320 |
13.99 |
9.33 |
67.29 |
T4b |
320 |
11.52 |
7.68 |
73.07 |
T4c |
320 |
11.21 |
7.47 |
73.79 |
T4d |
320 |
11.75 |
7.83 |
72.53 |
T4e |
320 |
12.09 |
8.06 |
71.73 |
T4 Mean |
|
12.11 |
8.07 (RT)* |
71.68 (IT)* |
|
|
|
|
|
T5a |
1000 |
0.55 |
0.37 |
98.70 |
T5b |
1000 |
2.23 |
1.49 |
94.78 |
T5c |
1000 |
1.56 |
1.04 |
96.34 |
T5d |
1000 |
0.83 |
0.56 |
98.05 |
T5e |
1000 |
0.61 |
0.41 |
98.56 |
T5 Mean |
|
1.16 |
0.77 (RT)* |
97.29 (IT)* |
R: Reference item, 3,5-dichlorophenol; T: Test item; #: The amount of suspended solids in the final test mixture was 1.5 g/L; RT: Total respiration with test item; IT: % inhibition of total respiration relative to RC; *Statistically significantly different compared to control
Table 12. Test Item - Results of probit analysis
Parameter |
EC50 |
EC20 |
EC10 |
Value [mg/L] |
194.32 |
92.97 |
63.24 |
lower 95%-cl |
164.91 |
68.75 |
41.98 |
upper 95%-cl |
228.74 |
114.28 |
82.42 |
Description of key information
Key study. Method according to OECD 209, GLP study. The test item has a 3h-EC50 = 194 mg/L and an EC10 = 63 mg/L in microorganisms. The NOEC was determined to be 32 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 194 mg/L
- EC10 or NOEC for microorganisms:
- 32 mg/L
Additional information
A study on the activated sludge respiration inhibition of the test item was carried out according to OECD 209 (GLP study). The final test was performed based on the result of a preceding combined limit/range-finding test. The respiration rates of samples of activated sludge from a sewage treatment plant treating predominantly domestic sewage was measured in an enclosed cell containing an oxygen electrode after a contact time (incubation) of 3 hours. Five concentrations were tested, ranging from 10 to 1000 mg/L and increasing with a factor 3.2. Five replicates per concentration and six replicates for an untreated control group were tested. An abiotic control was performed for all test groups to correct for the oxygen production of the test item (five replicates per group). Since the oxygen production observed in the abiotic control was above 5% in the highest concentration, the respiration rates of the test item are corrected with the respiration rate of the abiotic control from the corresponding concentration. The study met the acceptability criteria. The 3h-EC50 of the test item on aerobic waste water (activated sludge) bacteria was 194 mg/L (95% CL: 165 - 229 mg/L), the EC20 was 93 mg/L (69 - 114 mg/L) and the EC10 was 63 mg/L (42 - 82 mg/L). The test item was not toxic to waste water (activated sludge) bacteria at a concentration of 32 mg/L (NOEC).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.