N-methylacetamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (e.g. no analytical verification of test concentrations)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German Industrial Standard DIN 38412, part 11

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-methylacetamide

EC Number:

201-182-6

EC Name:

N-methylacetamide

Cas Number:

79-16-3

Molecular formula:

C3H7NO

IUPAC Name:

N-methylacetamide

Details on test material:

- test substance: N-Methylacetamide
- purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: German Federal Health Agency, Berlin
- breeding method: single breeding
- 1 animal in 100 ml beaker with 70 ml medium
- reproduction rate: 3 animals/d

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Details on test conditions:

- test medium: according to German Industrial Standard DIN 38412, part 11
- temperature: 21 °C
- test vessels: 50-ml glass beaker (DIN 12331)
- test volume: 20 ml (4 ml/animal)
- 5 animals per vessel
- 20 animals per concentration level (= 4 replicates)
- illumination: diffuse light (10 µE/m²*s)
- observation times: visually after 0, 3, 6, 24 and 48 h
- observation parameters: mobility
- test concentrations: 0 (control), 58, 100, 180, 320, and 580 mg/l
- reference substance: potassium dichromate
- EC 50 after 24 h and EC 50 after 48 h were determined according to Spearman-Kärber (Sachs, L. (1984): Angewandte Statistik, Springer, Berlin.)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

RS-Freetext:
Summary of test results
                  Results after 
              3h       6h       24h      48h
EC0 (mg/l)    >= 580   >= 580   >= 580   >= 580
EC50 (mg/l)   > 580   > 580     > 580    > 580
EC100 (mg/l)  > 580   > 580     > 580    > 580


The results were obtained at 21 °C, while the test
medium had the following specifications:
oxygen 8.0 mg/l
pH 7.42-7.67


Test results:

Immobility:
Nominal                   Cumulative immobility (n)
Concentration (mg/l)   0 h   3 h    6 h   24 h   48 h
   0 (Control)          0     0      0      0      0 
 100                    0     0      0      0      0 
 180                    0     0      1      1      1 
 320                    0     0      0      0      0 
 580                    0     0      1      1      1

Reference substance:
EC0:   1.0 mg/l
EC50:  1.45 mg/l
EC100: 3.2 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes