Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-317-6 | CAS number: 57-15-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15-Sep-2017 to 09-Mar-2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- The tempereture values were partially outside the range given in the study plan and thus represents a deviation of the study plan which is not considered to have an influence an the
integrity of the test. The whole temperature range is in accordance with the OECD Test Guideline
201. - Deviations:
- yes
- Remarks:
- see remarks
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,1,1-trichloro-2-methyl-2-propanol hemihydrate
- Cas Number:
- 6001-64-5
- Molecular formula:
- C4H7Cl3O H2O
- IUPAC Name:
- 1,1,1-trichloro-2-methyl-2-propanol hemihydrate
- Test material form:
- solid: crystalline
- Details on test material:
- transparent and crystalline solid at ambient temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- GC-MS
- Details on sampling:
- Specimens were drawn from freshly prepared treatments and those incubated for 72 hrs. The supporting analysis of the specimens could not be performed on the same day of collection of the specimens. Therefore, all specimens from freshly prepared test solutions and those incubated for 72 hrs were stored deep-frozen at <= -18°C in glass bottles and analysed after thawing.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- An aliquot of the algal stock culture was diluted with test medium to an absorbance value of E578 nm = 0.015 of the algal suspension giving an initial algal cell concentration of 3*103 cellslml. The determination of cell concentration was performed by photometrical measurement using a calibration curve prepared by using a spectrophotometer at 578 nm. The test item was prepared as follows: Separate solutions (see below) were made for each test concentration in ultrapure water. Aliquots of these solutions were made up with "intermediate dilution" (see section above) and algal inoculum (or diluted "intermediate dilution") to give 50 ml of final volume. The test item was introduced into the test solutions using individual stock solutions for each concentration (screening). The solutions were used directly without any further dilution step. By the addition of the "intermediate dilution" of minerar nutrients and the algal inoculation the final nominal concentrations were achieved. For the inoculated test solutions with the test item, as well as those without any test item, 7 parallals (main test) were incubated under constant light. As reagents' Blank without algal inoculation three test solutions were prepared containing a 1:10 diluted intermediate dilution of the mineral nutrients stock solution instead of the algal pre-culture. Theseblanks were incubated under the same conditions under constant light as the test solution to be tested. The measured turbidity in those test solutions was subtracted from those with the algal inoculation. ln the same way, seven control solutions without any test item but with algal inoculation were prepared as weil as one test solution without any test item and without algal inoculation.
Test organisms
- Test organisms (species):
- other: Desmodesmus subspicatus CHODAT
- Details on test organisms:
- Origin: Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen [SAG], Strain-No. 86.81.
Study design
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- Temperature was controlled by means of a min/max-Thermometer and registered additionally by means of a data Iogger. The temperature range registered by the data Iogger is documented below as a graph. The temperature measured during this test was in the range of 24.6 and 25.3°C (mean 24.9°C) and thus in the required range of the OECD-test guideline.
- pH:
- 8.1 - 9.6
pH values increased during the course of the study upon growth of the algae. This increase of pH in the controls by 1.41 units was in the required range of s 1.5 units indicated in OEGD test guideline 201. - Nominal and measured concentrations:
- 0 and 100 mg/l
As a result of the supporting analyse it can be stated that the measured concentration of nominally 100 mg/L remained stable >98% during incubation and thus, the results of the biological part should be based on the nominal concentrations applied because the necessary stability of ~ 80% could be shown. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The validity criteria for the study as given by the OECD-Test Guideline 201 were all met and the study is therefore considered to be valid.
EC50 > 100 mg/l - Executive summary:
The toxicity of Chlorobutanol Hemihydrate towards algae was tested according to OECD-Test Guideline No. 201, in the Version dated 23-March-2006. The toxic effect was investigated by determination of the inhibition of the growth rate of the algae and the yield during the exposure period of 72 hours. The test was performed as a Iimit test using 100 mg/L as the sole nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.