Registration Dossier
Registration Dossier
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EC number: 205-760-9 | CAS number: 150-46-9
Ecotoxicological information
Short-term toxicity to aquatic invertebrates
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Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Remarks:
- No data is available on the GLP compliance
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 90 mg/L
- Test temperature:
- 24.0 °C
- pH:
- mean = 7.4. control = 7.8, highest boron concentration = 6.8
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: 0.64, 31, 62, 125, 245, 490 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002)
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 5.40
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 102 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 82-125 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 91 mg/L
- Test temperature:
- 24.3 °C
- pH:
- 8.0
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: 0.6, 35, 69, 135, 260, 540 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 165 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 137-198 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 89 mg/L
- Test temperature:
- 25.0 °C
- pH:
- 8.1
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: <0.047, 36, 72, 135, 280, 535 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 109 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 93-128 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 282 mg/L
- Test temperature:
- 25.0 °C
- pH:
- 8.1
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: <0.02, 34, 70, 135, 265, 530 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make hardness = 300 mg/L (added MgSO4 and CaSO4 in same proportions as standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 104 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 87-123 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 469 mg/L
- Test temperature:
- 25.0 °C
- pH:
- 8.1
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: <0.02, 35, 70, 140, 265, 530 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make hardness = 500 mg/L (added MgSO4 and CaSO4 in same proportions as standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 93 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 77-114 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 85 mg/L
- Test temperature:
- 25.0 °C
- pH:
- 6.7
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 18.75, 37.5, 75, 150, 300 mg-B/L
Measured: 0.03, 21, 44, 84, 170, 335 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make pH = 6.5 (reduced NaHCO3 to 4 mg/L rather than 96 mg/L like standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 91 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 79-106 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 87 mg/L
- Test temperature:
- 24.9 °C
- pH:
- 7.6
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 18.75, 37.5, 75, 150, 300 mg-B/L
Measured: 0.03, 19, 44, 88, 160, 315 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make pH = 7.5 (reduced NaHCO3 to 39.8 mg/L rather than 96 mg/L like standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 115 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 108-122 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Each treatment was sampled at beginning and end of test
- Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 84 mg/L
- Test temperature:
- 25.0 °C
- pH:
- 8.4
- Dissolved oxygen:
- > 6.0 mg/L
- Nominal and measured concentrations:
- Nominal: Control, 25, 50, 100, 200, 400 mg-B/L
Measured: 0.03, 26, 56, 110, 210, 425 mg-B/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make pH = 8.5 (increased NaHCO3 to 398.5 mg/L rather than 96 mg/L like standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily) - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 142 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: (95% CI 130-155 mg-B/L)
- Reported statistics and error estimates:
- LC50 calculated using trimmed Spearman-Karber method
Referenceopen allclose all
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| 0.64 (control) | 2 | 10.0% |
| 31 | 2 | 10.0% |
| 62 | 9 | 45.0% |
| 125 | 9 | 45.0% |
| 246 | 20 | 100.0% |
| 490 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| 0.6 (control) | 2 | 10.0% |
| 35 | 5 | 25.0% |
| 69 | 4 | 20.0% |
| 135 | 6 | 30.0% |
| 260 | 20 | 100.0% |
| 535 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| <0.047 (control) | 1 | 5.0% |
| 36 | 2 | 10.0% |
| 72 | 2 | 10.0% |
| 135 | 15 | 75.0% |
| 280 | 20 | 100.0% |
| 535 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| <0.02 (control) | 2 | 10.0% |
| 34 | 0 | 0.0% |
| 70 | 3 | 15.0% |
| 135 | 15 | 75.0% |
| 265 | 20 | 100.0% |
| 530 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| <0.02 (control) | 2 | 10.0% |
| 35 | 2 | 10.0% |
| 70 | 4 | 20.0% |
| 140 | 17 | 85.0% |
| 265 | 20 | 100.0% |
| 530 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| 0.03 (control) | 0 | 0.0% |
| 21 | 0 | 0.0% |
| 44 | 0 | 0.0% |
| 84 | 8 | 40.0% |
| 170 | 20 | 100.0% |
| 335 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| 0.03 (control) | 0 | 0.0% |
| 19 | 0 | 0.0% |
| 44 | 0 | 0.0% |
| 88 | 1 | 5.0% |
| 160 | 20 | 100.0% |
| 335 | 20 | 100.0% |
Data
| Concentration (mg B/L) | # dead (out of 20) | Percent |
| 0.03 (control) | 0 | 0.0% |
| 26 | 0 | 0.0% |
| 56 | 0 | 0.0% |
| 110 | 2 | 10.0% |
| 210 | 20 | 100.0% |
| 425 | 20 | 100.0% |
Description of key information
Short-term effects on freshwater invertebrates ranged from 64 to >544 mg B/L.
Adequate short-term LC50 of 130 mg B/L was found for the marine shrimp L. vannamei.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 64 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 130 mg/L
Additional information
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