Registration Dossier

Administrative data

Description of key information

The substance is not skin irritant but it causes serious eye damage.

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Eye irritation

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (irreversible damage)

Respiratory irritation

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Skin irritation/corrosion

Tetraphosphorus trisulphide was tested for its skin irritation potential with an in vitro test according to OECD TG 439 Reconstructed human epidermis model. The mean cell viability of test item treated tissues was 106 % after 15 minutes exposure period and 42 hours of post-exposure incubation period. No classification for skin irritation of this substance is required according to the CLP Regulation (EC.n.1272/2008).

Eye damage

Tetraphosphorus trisulphide was tested for its eye irritation potential with an in vitro test according to OECD TG 492 Reconstructed human Cornea-like Epithelium (RhCE) test method. After 6 hours of exposure followed by product washing and a post incubation of 18 hours a residual cell viability (mean of two replicates) of 15.1 % has been quantified for test item. This results did not allow discrimination between eye irritation/reversible effects on the eye (Category 2) and serious eye damage/irreversible effects on the eye (Category 1). For this purpose, further investigation were performed. Tetraphosphorus trisulphide was tested for its eye damage potential with an in vitro test according to OECD TG 437 Bovine Corneal opacity and Permeability test (BCOP). The test item was incubated on the cornea for 4 hours at 32 ± 1 °C and, after removal of the test item, opacity and permeability values were measured. The calculated IVIS value for the test item was 95.56 (> 55) and so it is possible to conclude that Tetraphosphorus trisulphide causes serious eye damage on the cornea of the bovine eye and it is classified H318 (eye damage cat.1) according to the CLP Regulation (EC. n.1272/2008).

Justification for classification or non-classification

According to the OECD TG 439 (EpiSkinTM/MTT Method) the test item is classified not irritant to skin if the mean relative viability is higher than 50 % according to the CLP Regulation (EC n.1272/2008). Tetraphosphorus trisulphide was tested for skin irritation according to OECD TG 439: the mean cell viability of the test item treated tissues was 106 % and therefore no classification for skin irritation/corrosion is required according to the CLP Regulation (EC n.1272/2008).

According to OECD TG 437 Bovine Corneal Opacity and Permeability (BCOP) Test, a substance with an IVIS > 55 induces serious eye damage and it is classified UN GHS Category I or H318 (eye damage cat.1) according to the CLP Regulation (EC n.1272/2008). IVIS of Tetraphosphorus trisulphide is 95.56 and it satisfies the criteria for the classification H318 (eye damage cat.1) according to the CLP Regulation (EC n.1272/2008).