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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The Metabolism in the Rat of Photosynthetically Prepared Myo-Inositol-C14
Author:
Moscatelli EA and Larner J
Year:
1959
Bibliographic source:
Arch. Biochem. Biophys., 80:26-34

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The pathway of carbon in test substance biosynthesis and dissimilation along 3 pathways (oxidation to CO2; incorporation into lipides of brain, liver, kidney, and heart; and conversion to liver glycogen) in the intact, normal rat.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Purity: not reported
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
not specified
Sex:
female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
not specified
Doses / concentrationsopen allclose all
Dose / conc.:
21.6 other: mg
Remarks:
In experiment No. 1, the first dose was 897000 disintegrations/min of the carbon-14 labled test substance with a reactivity of 41500 disintergrations/mg. The dose in mg was determined by dividing 897000 by 41500 to yield 21.6 mg.
Dose / conc.:
24.5 other: mg
Remarks:
In experiment No. 1, the second dose was 1015000 disintegrations/min of the carbon-14 labled test substance with a reactivity of 41500 disintergrations/mg. The dose in mg was determined by dividing 1015000 by 41500 to yield 24.5 mg.
Dose / conc.:
83.3 other: mg
Remarks:
In experiment No. 2, the total dose was 2090000 disintegrations/min of the carbon-14 labled test substance with a reactivity of 25100 disintergrations/mg. The dose in mg was determined by dividing 2090000 by 25100 to yield 83.3 mg.
Dose / conc.:
33.9 other: mg
Remarks:
In experiment No. 3, the total dose was 4,200,000 disintegrations/min of the carbon-14 labled test substance with a reactivity of 124,000 disintergrations/mg. The dose in mg was determined by dividing 4,200,000 by 124,000 to yield 33.9 mg.
No. of animals per sex per dose:
1
Control animals:
no
Positive control:
no

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on excretion:
The yields of CO2 were 25.7% for the Experiment 1 rat (starved), 22.3% for the Experiment 2 rat (starved), and 10.1% for the Experiment 3 rat (fed). Higher recoveries of CO2 were found in the starved rats. Recoveries in the urine were 22.8, 56.0, and 6.2% In Experiments 1, 2, and 3, respectively. Almost 50% of the administered radioactive dose was found in the carcass of the rat in Experiment 3; an over-all recovery of approximately 66% of the administered radioactive dose.

Applicant's summary and conclusion

Conclusions:
The test substance is metabolized to CO2 by the rat, approximately 25% of the administered dose being excreted into the respiratory CO2.
Executive summary:

Three pathways of test substance metabolism [(a) oxidation to CO2; (b) incorporation into lipids of brain, liver, kidney, and heart; and (c) conversion to liver glycogen] in the whole rat were quantitatively investigated. A small conversion to glucose is shown by liver glycogen labeling. Comparable amounts are incorporated into organ lipids. A much larger conversion is represented by a vigorous oxidative dissimilation to CO2. The largest total incorporations were found in the lipides from liver and kidney.