Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-293-0 | CAS number: 137-42-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source.
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Genetic toxicity in vitro for Metam Sodium in Salmonella typhimurium by Standard plate test
- Author:
- European Commission
- Year:
- 2 000
- Bibliographic source:
- European Commission, European Chemicals Bureau, 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- To evaluate the genetic toxicity of Metam Sodium in Salmonella typhimurium TA92, TA98, TA100, TA1535, TA1537, TA1538 by Standard plate test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Metam-sodium
- EC Number:
- 205-293-0
- EC Name:
- Metam-sodium
- Cas Number:
- 137-42-8
- Molecular formula:
- C2H5NS2.Na
- IUPAC Name:
- sodium (methylcarbamothioyl)sulfanide
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material : Metam-sodium
- Molecular formula : C2H4NNaS2
- Molecular weight : 129.1826 g/mol
- Smiles notation : C(=S)(NC)[S-].[Na+]
- InChl : 1S/C2H5NS2.Na/c1-3-2(4)5;/h1H3,(H2,3,4,5);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material : Metam-sodium
- Molecular formula : C2H4NNaS2
- Molecular weight : 129.1826 g/mol
- Smiles notation : C(=S)(NC)[S-].[Na+]
- InChl : 1S/C2H5NS2.Na/c1-3-2(4)5;/h1H3,(H2,3,4,5);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA92, TA98, TA100, TA1535, TA1537, TA1538
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 metabolic activation
- Test concentrations with justification for top dose:
- 20–5000 µg/plate
- Vehicle / solvent:
- not specified
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
Exposure duration: 48 hours
NUMBER OF REPLICATIONS: Duplicate
Other: Triplicate plates per test were used. - Rationale for test conditions:
- not specified
- Evaluation criteria:
- Evaluation was done considering a dose dependent increase in the number of revertants /plate.
- Statistics:
- not specified
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA92, TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: No mutagenic effect were observed.
Applicant's summary and conclusion
- Conclusions:
- Metam Sodium (137-42-8) was evaluated for its mutagenic potential in Salmonella typhimurium TA92, TA98, TA100, TA1535, TA1537 and TA1538 by Standard plate test. The test result was considered to be negative in the presence and absence of metabolic activation.
- Executive summary:
Genetic toxicity study for Metam Sodium was assessed for its mutagenic potential. For this purpose Salmonella typhimurium Reverse Mutation Assay was performed according to OECD Guideline 471. The test substance was exposed to Salmonella typhimurium TA92, TA98, TA100, TA1535, TA1537 and TA1538 by Standard plate test at the concentration of 20–5000 µg/plate. The test substance was exposed for 48 hour in the presence and absence of metabolic activation. No mutagenic effects were observed. Therefore Metam Sodium was considered to be non mutagenic for Salmonella typhimurium TA92, TA98, TA100, TA1535, TA1537 and TA1538 by Standard plate test. Hence the substance cannot be classified as gene mutant in vitro.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
