Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non standard protocol, but well described method

Data source

Reference
Reference Type:
publication
Title:
Effect of 3,4-Dichloroaniline and Metavanadate on Daphnia Populations.
Author:
Nelly Van Der Hoeven
Year:
1989
Bibliographic source:
Ecotoxicology and Environmental Safety 20:53-70 (1990)

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
A Daphnia magna population was exposed to metavanadate for up to 14 weeks, under continuous flow through conditions. The population size, length and eppiphia production were observed.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no detail on test material was described
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
vanadate: RNCAS: 13981-20-9
MW: 98.9
formula: VO3-

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
The medium was groundwater out of the neighborhood of Linschoten, obtained directly from a water company. The mineral composition was adjusted to a standard:
Na(+) = 27,4 mg/L
K (+) = 7,8 mg/L
Ca (2+) = 54,4 mg/L
Mg (2+) = 17,5 mg/L
Cl (-) = 96,6 mg/L
SO4 (2-) = 70,1 mg/L
HCO3 (-) = 84,8 mg/L
pH: 8.1

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
All experiments were started with 5 egg-bearing females D. magna, 10 days old.

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 wk

Test conditions

Test temperature:
22°C
pH:
8.1
Nominal and measured concentrations:
0.56, 1 and 1.8 mg V/L
Details on test conditions:
TEST SYSTEM

- Material, size, headspace, fill volume: 2L conical flasks, volume of medium: 800mL
- Aeration: constant, slow
- Renewal rate of test solution: flow rate: constant at 1 liter/ day
vessels cleaned and refilled on Mondays, Wednesdays and Fridays. On the other days they were filled with fresh medium.
The inflow medium contained 400.000.000 C. pyrenoidosa cells and 500.000.000 yeast cells per liter.
- Photoperiod: 14 h light/ 10 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : population count, mean lenght, ephippial eggs count, every week
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 wk
Dose descriptor:
NOEC
Effect conc.:
0.56 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Remarks:
vanadate or vanadium element
Basis for effect:
reproduction
Duration:
14 wk
Dose descriptor:
NOEC
Effect conc.:
1 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Remarks:
vanadate (or vanadium element)
Basis for effect:
mortality
Reported statistics and error estimates:
no data

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

A population of Daphnia magna was exposed to metavanadate during 14 weeks under continuous flow through conditions. Three concentrations of the toxicant were used: 0.56, 1.0 and 1.8 mg/L. Effects of metavanadate were observed every week: population dynamic, mean lenght of daphnids and production of eggs. The test was carried out under standard protocol, but the methodology and the dilution medium were well described.

A 14 weeks NOEC value of 0.56 mg V/L was shown for the effect of metavanadate on ephippia production and a NOEC of 1.0 mg V/ L was observed for mortality (promotion effect up this concentration).