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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study not GLP but in which all parameters described are comparable to guideline methods for testing acute toxicity with invertebrates.
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Toxicity of Vanadium to Different Freshwater Organisms.
Author:
Beusen J.M. and Neven B.
Year:
1987
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology, 39, 194-201.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Remarks:
even if data such as hardness, O2 or photoperiod are lacking
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified
Details on test material:
purity > 98%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified
Details on test solutions:
Dilution water used for stock cultures and tests is synthetic hard water:
composition (mg/L deionized water):
100 mg NaHCO3,
20 mg KHCO3,
180 mg MgSO4.7H2O
200 mg CaCl2.2H2O

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: daphnids
- Source: obtained from a standardized laboratory culture originally obtained from an uncontaminated pond in the vicinity of the laboratory.
- Age at study initiation: less than 24h old
- Feeding: yes, fed daily with green algae (Chlorella pyrenoidosa)
The stock cultures are held in 3 l beakers containing gently bubble-aerated hard water with a temperature of 19 ± 1°C.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
223 mg/l (as CaCO3 + MgSO4)
Test temperature:
19 °c +/- 1°C
pH:
8.2 - 8.4
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
Not given, but the measured vanadium concentrations corresponded within 10% of the initial amount of the metal added to each test container.
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 mL borosilicate glass beakers
- Aeration: no aeration during the test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

EFFECT PARAMETERS MEASURED:
- mortality: determined by lack of movement of antennae or post-abdominal claw on gentle prodding
- complete immobilization: determined by unability of swimming for 15 seconds after gentle agitation

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
4 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: mean of five replicates
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Basis for effect:
mobility
Remarks on result:
other: mean of five replicates

Any other information on results incl. tables

table 1: summary of results of acute toxicity to Daphnia magna of vanadium (concentrations in mg V/L)

Run

Mortality : 48h LC50

Confidence interval

Mobility : 48h EC50

Confidence interval

1

4.8

4.4- 5.3

3.8

Not calculated

2

3.9

3.1- 4.9

2.9

2.3- 3.7

3

4.3

3.5- 5.3

3.9

3.3- 4.6

4

3.9

2.7- 5.6

3.6

3.0- 4.2

5

3.4

2.7- 4.3

3.3

2.6- 4.0

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

Daphnia magna were exposed to 5 measured vanadium concentrations during 48h under static conditions and in a way similar to the guidelines. Mortality and immobilisation were assessed, and differences between these parameter values were very small. The 48h-NOEC for mobility was determined to be 3.5 mg V/L(geometric mean of 5 replicates).