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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: study not GLP but in which all parameters described are comparable to guideline methods for testing acute toxicity to fish
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Toxicity of Vanadium to Different Freshwater Organisms.
Author:
Beusen J.M. and Neven B.
Year:
1987
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology, 39, 194-201.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Remarks:
even if not all data are indicated
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified
Details on test material:
purity > 98%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified
Details on test solutions:
Dilution water used for stock cultures and tests is synthetic hard water:
composition (mg/L deionized water):
100 mg NaHCO3,
20 mg KHCO3,
180 mg MgSO4.7H2O
200 mg CaCl2.2H2O

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Brachydanio rerio are obtained from a commercial supply house.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
no data
Test temperature:
23°C +/-2°C
pH:
7.7 to 8.5
Dissolved oxygen:
above 6 mg/L
Salinity:
not given
Nominal and measured concentrations:
Not given, but the measured vanadium concentrations were within 10% of the initial amount of the metal added to each test vessel.
A control plus at least 5 concentrations were selected on a basis of a logarithmic expansion.
Details on test conditions:
Before testing, fishes from a single stock of similar lenght and age are adapted to laboratory conditions in 200 L tanks filled with hard water for at least 12 days. They were fed twice daily, ceasing 24h before initiation of the test.
photoperiod: 14h daylight/ 10h darkness

TEST SYSTEM
- Test vessel: 1L beakers filled with 1L of test solution
- fishes are transferred to new test medium after 48 and 96h
- Aeration: gentle bubble aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): duplicate

Before testing, fishes from a single stock of similar lenght and age are adapted to laboratory conditions in 200 L tanks filled with hard water for at least 12 days. They were fed twice daily, ceasing 24h before initaition of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14h daylight/ 10h darkness

EFFECT PARAMETERS MEASURED: mortalities, at 24h intervals
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: geometric mean of 3 data
Details on results:
the 96h LC50 data are as follows:
2.9 (CI: 2.1- 3.4)
4.1 (CI: 2.6- 6.4)
5.3 (CI: 3.4- 8.4)

Applicant's summary and conclusion

Executive summary:

Zebrafish; Danio rerio were exposed to a control and at least 5 measured vanadium concentrations during 96h under static conditions and in a way similar to the guidelines. The 96h-LC50 for mortality was determined to be 4 mg V/L (geometric mean of 3 replicates).