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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23.05.2017 - 26.05.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Lithium perchlorate
Purity: 98.7 % approx.
Batch No.: BCBN2047V
Analytical monitoring:
yes
Details on sampling:
Analysis of the test media samples was conducted on fresh media at 0 hours and on corresponding old media at 72 hours. Measured test concentrations were determined from samples of test solution from each treatment and control group.
Vehicle:
no
Details on test solutions:
Test vessels (250 mL conical flasks) were prepared containing 100 mL of the appropriate test or control medium. The test concentration was prepared by 10 minutes stirring. Each test vessel was inoculated with 1 × 104 algae cells/mL and incubated at 23.3 – 24.6°C under a mean light intensity of 5401 Lux for 72 hours. Algae cell counts were conducted at 24-hour intervals. All test concentrations were visually clear at the start of the test. No precipitation of test substance was observed during the test.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
The test organism, Pseudokirchneriella subcapitata (Strain 278/4), was originally obtained from the Culture Collection of Algae and Protozoa (CCAP)
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Hardness:
98 mg CaCO3/L
Test temperature:
23.3 - 24.6 °C
pH:
7.65 - 9.07
Nominal and measured concentrations:
Nominal: 120 mg/L
Measured: 105 mg/L (at 0 hours) - 126 mg/L (at 72 hours)
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Description of key information

Inhibition of Growth:

ErC50 (72 h): > 120 mg/L

NOEC (72 h): > 120 mg/L

Key value for chemical safety assessment

Additional information