N,N'-methanetetraylbis(1-methylethylamine)

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

A primary irritancy study on the carbodiimides was conducted to establish sensitization and challenge concentrations and to ensure the mice would tolerate multiple days of topical exposure. This assay was a modification of the phototoxicity murine model developed by Gerberick and Ryan.**

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-methanetetraylbis(1-methylethylamine)

EC Number:

211-743-7

EC Name:

N,N'-methanetetraylbis(1-methylethylamine)

Cas Number:

693-13-0

Molecular formula:

C7H14N2

IUPAC Name:

(propan-2-yl)({[(propan-2-yl)imino]methylidene})amine

Test material form:

liquid

Test animals

Species:

mouse

Strain:

B6C3F1

Details on test animals or test system and environmental conditions:

Female B6C3F1 mice (4-5 weeks, 17-20 grams) were purchased from Taconic Farms (Germantown, NY) hepatitis and Sendai virus free and quarantined until 8 weeks of age. The mice were maintained on Agway Rat and Mouse Ration (NIH 031) and tap water ad libitum. The animal facility maintained a temperature between 18-26"C, a relative humidity between 40-70%, and a lightfdark cycle of 12-hour intervals throughout the experimental period.

Test system

Type of coverage:

not specified

Preparation of test site:

not specified

Vehicle:

other: acetone

Controls:

yes, concurrent vehicle

Amount / concentration applied:

With a pipette, 12.5 pl of the test material or vehicle was applied to both sides of each ear pinna for four consecutive days (50 pllmousdday).

Duration of treatment / exposure:

4 days

Observation period:

Twenty-four hours (j2 hr) after the final treatment, mice were restrained and ear thickness was measured in the identical manner as pretreatment measurements

Number of animals:

no data

Results and discussion

In vivo

Results

Irritation parameter:

other: ear thickness

Basis:

other: The mean percent ear swelling

Time point:

24 h

Remarks on result:

positive indication of irritation

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

It is classifed as Skin Irrit. 2 H315

Executive summary:

Animals exposed to DIC experienced dose dependent irritation responses. For DIC, the Maximal Non-Irritating Concentration (MNC) was 3% and the Minimal Irritating Concentration (MIC) was 10%.