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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
A Comparison of the Half-life of Inorganic and Organic Tin in the Mouse
Author:
Brown, R.A., C.M. Nazario, R.S.de Tirado
Year:
1977
Bibliographic source:
Environmental Research 13, 56-61

Materials and methods

Objective of study:
excretion
Principles of method if other than guideline:
A small quantity of 113Sn in 4M HCl was diluted into phosphate buffered saline containing isologous 10% normal mouse serum. One-half milliliter of this solution was injected intraperitoneally into each of five Swiss mice. Six days after injection of the labeled tin solution, 0.5 mL, containing 80 ug of stannous chloride, was injected into two of the five mice.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): radioactive tin
Radiolabelling:
yes

Test animals

Species:
mouse
Strain:
Swiss
Sex:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS

- Diet (e.g. ad libitum): normal laboratory chow
- Water (e.g. ad libitum): throughout experiment

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
unchanged (no vehicle)
Details on exposure:
A small quanity of 113Sn in 4M HCl was diluted into phosphate buffered saline containing isologous 10% normal mouse serum. One-half milliliter of this solution was injected intraperitoneally into each of five Swiss mice. Six days after injection of the labeled tin solution, 0.5 mL, containing 80 ug of stannous chloride, was injected into two of the five mice.
Duration and frequency of treatment / exposure:
Six days after the 113Sn solution was injected, stannous chloride was injected
Doses / concentrations
Remarks:
Doses / Concentrations:
0.5 mL containing 80 ug of stannous chloride
No. of animals per sex per dose:
Five mice were injected with 113Sn. Six days later stannous chloride was injected into two of the mice.
Control animals:
no
Details on dosing and sampling:
The radioactivity of the mice was counted in a whole-body counter facility consisting of NaI crystals, each 6 in. in diameter. Two crystals were mounted from the roof of the steel room housing them and the other two crystals were mounted from the floor. Each mouse was counted between two crystals, one upper and one lower. It was confined to the central region of the crystals in a small cylindrical chamber attached to a plastic frame which fitted over the lower crystal and also served as a spacer for the crystals. This arrangement permitted an accurately reproducible and almost 4 pi counting geometry.
The system was calibrated so that 512 channels represented 512 keV. The accumulated counts in all channels in the region of the mean photopeaks of labeled tin and indium, its decay product, were printed out.

Results and discussion

Main ADME results
Type:
excretion
Results:
Curve fitting by least squares gave an effective half-life of 25 days for 113Sn activity and 21 days for labeled indium, with coefficients of determination of 0.93 and 0.80, respectively.

Toxicokinetic / pharmacokinetic studies

Details on excretion:
The injection of the unlabeled tin into two mice which had been injected with 113Sn had no apparent effect upon the observed whole-body counts. The decrease in whole-body counts with time was the same for both injected and noninjected mice containing labeled inorganic tin. Labeled tin has a half-life of 118 days and labeled indium, 1.7 hours.
Toxicokinetic parameters
Test no.:
#1
Toxicokinetic parameters:
half-life 1st: 29 days

Any other information on results incl. tables

The injection of unlabeled tin into mice which had been injected with 113Sn 6 days earlier had no apparent effect upon the observed whole-body counts. The decrease in the whole-body counts with time was the same for both injected and noninjected mice containing labeled inorganic tin.

Twenty-three days for 113Sn activity based on the average of the observed values for labeled tin and labeled indium. This half-life is the result of the biological and physical decay. Using 118 days for the physical half-life, it is estimated that the biological half-life in the mouse would be 29 days.

Applicant's summary and conclusion

Conclusions:
It is estimated that the effective half-life of labeled tin in the mouse to be 23 days, the average of the observed values for labeled tin and labeled indium. This half-life is the result of the biological and physical decay. Using 118 days for the physical half-life, it is estimated that the biological half-life in the mouse would be 29 days.
The injection of unlabeled tin into mice which had been injected with radiolabeled tin 6 days earlier had no apparent effect upon the observed whole-body counts. The decrease in the whole-body counts with time was the same for both injected and noninjected mice containing labeled inorganic tin.
Executive summary:

The injection of the unlabeled tin into two mice which had been injected with 113Sn 6 days earlier had no apparent effect upon the observed wholebody counts. The decrease in the wholebody counts with time was the same for both injected and noninjected mice containing labeled inorganic tin.