Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The pharmacokinetic study on the fate of 8-hydroxyquinoline in rat
Author:
Kiwada H
Year:
1977
Bibliographic source:
Chem. Phar. Bull. 25: 1566-1573

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Propylene glycol solution for 8-hydroxyquinoline was administered intravenously through the femoral vein. Bile and urine samples were collected at approproate time intervals until 8 hr through polyethylene tubes set into bile duct, i.e bile fistula, and bladder, respectively. After 14C-8-hydroxyquinoline in propylene glycol solution 4.86 mg containing 10.1 uCi per head was administered intravenously, blood samples were collected from femoral artery through polyethylene cannula at appropriate time intervals until 4 hr.
GLP compliance:
no

Test material

Radiolabelling:
yes

Test animals

Species:
rat
Strain:
other: Dondryu
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
propylene glycol
Doses / concentrations
Dose / conc.:
15 mg/kg bw/day (nominal)
No. of animals per sex per dose / concentration:
Three
Control animals:
no

Results and discussion

Main ADME resultsopen allclose all
Type:
absorption
Results:
Intraperitoneal administration of 8-hydroxyquinoline showed rapid absorption in all animals at 8 h (>80%) based on urinary excretion.
Type:
metabolism
Results:
Glucuronide about 65% of dose and suphate about 25% of dose. No hydrolysis of the 8- hydroxyquinoline main metabolites occurred in vivo.
Type:
excretion
Results:
82.8% of the test material was eliminated in urine and 8.7% in bile within 8 h.

Toxicokinetic / pharmacokinetic studies

Details on absorption:
Intraperitoneal administration of 8-hydroxyquinoline showed rapid absorption in all animals at 8 h (>80%) based on urinary excretion.
Details on excretion:
82.8% of the test material was eliminated in urine and 8.7% in bile within 8 h.

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
Glucuronide about 65% of dose and suphate about 25% of dose. No hydrolysis of the 8- hydroxyquinoline main metabolites occurred in vivo.

Applicant's summary and conclusion

Executive summary:

Two metabolites were found in male rat urine and bile after intravenous administration of 15 mg/kg bw of 8-hydroxyquinoline within 8 hours. 8-hydroxyquinoline glucuronide conjugate was collected in urine (59.9%) and bile (8.7%) and 8-hydroxyquinoline sulphate accounted for only in urine (22.9%). Unmetabolized 8-hydroxyquinoline was hardly detected both in urine and bile.

The fate of the in vivo glucuronide conjugate was followed after intravenous administration to rats and about 90% and 10% of the dose were excreted in urine as the same conjugate. In the same way the sulphate metabolite was administered intravenously in vivo and 95% was detected in urine but not in bile. These results showed that no hydrolysis of the 8- hydroxyquinoline main metabolites occurred in vivo.