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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17 Oct - 19 Oct 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
GLP compliance:
yes
Specific details on test material used for the study:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In a refridgerator
- Stability under test conditions: Verified by IR before exposure and at the end of the exposure

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 1.0, 1.8, 3.2, 5.6, and 10 mg/mL
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Incorporated Administrative Agency, National Institute for Environmental Studies, Tsukuba, Japan
- Age of parental stock (mean and range, SD): between 0 - 24 h
- Feeding during test: no food
- Medium: M4-Medium


ACCLIMATION
- Acclimation period: 26 Sep - 17 Oct 2001
- Type and amount of food: Chlorella vulgaris (0.15 - 0.2 mgC/organism/day)
- Health during acclimation (any mortality observed): Mortality of parental animals was below 5% during acclimation period.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
19.7 - 20.2 °C
pH:
8.0 - 8.1
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
Nominal concentration: 1.0, 1.8, 3.2, 5.6 and 10 mg/L
Geometric mean concentration during 48 hours: 1.09, 1.88, 3.45, 5.63 and 10.9 mg/L
Details on test conditions:
TEST SYSTEM
- Material, fill volume: Glass, 100 mL
- Aeration: no data
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the guideline
- Culture medium different from test medium: same medium
- Intervals of water quality measurement: the content of the test item, pH and concentration of dissolved oxygen were measured at the beginning and at the end of the test

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : after 24 and 48 h the immobilised Daphnids were counted

RANGE-FINDING STUDY
- Test concentrations: yes, without analytics, not further data in the report
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
2.89 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: no

Table 1. Calculated EiC50 values

Exposure period (hours)

EiC50

(mg/L)

95% confidence limits

(mg/L)

Statistical method

24

2.89

2.50 – 3.29

Probit

48

2.40

2.04 – 2.83

Probit

 

Table 2. Measured concentration of the test substance in test water

Nominal concentration (mg/L)

Measured concentration (mg/L)

Geometric mean during 48 hours

0 hour

new

Percent of nominal

48 hours

old

Percent of nominal

Control

< 0.003

-

< 0.003

-

-

1.0

1.19

119

1.00

100

1.09

1.8

2.00

111

1.76

98

1.88

3.2

3.78

118

3.14

98

3.45

5.6

6.29

112

5.04

90

5.63

10

11.6

116

10.3

103

10.9

Description of key information

EC50(48 h) = 2.4 mg/L (geometric mean measured, OECD 202, D. magna)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
2.4 mg/L

Additional information

There is one GLP study available investigating the acute immobilization of the substance towards aquatic invertebrates according to the OECD guideline 202 (1984).

In a static test, D. magna was exposed to the nominal concentrations 1.0, 1.8, 3.2, 5.6, and 10 mg/L test substance for 48 h under controlled conditions. A negative control was run in parallel. Test concentrations were analytically verified by HPLC.

The geometric mean measured concentrations of the test substance after 48 h were 1.09, 1.88, 3.45, 5.63, and 10.9 mg/L indicating that the substance was fully dissolved and stable over the duration of the test. The reported effect concentrations were based on the geometric mean measured concentrations. The EC50 (24 h) was 2.89 mg/L and the EC50 (48 h) was 2.4 mg/L.