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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD guideline followed to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
test volume increased and closed test vessels used to minimise loss of test substance by volatilisation
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
test volume increased and closed test vessels used to minimise loss of test substance by volatilisation
GLP compliance:
yes (incl. certificate)
Remarks:
Copy of certificate included

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
97.91% purity, manufactured in 1998 with a stability of >2 years
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples taken at start and end of exposure period

Test solutions

Vehicle:
no
Details on test solutions:
For the prepartation of a stock solution, appr. 2ml of the test substance were mixed with 200ml deionised water and occasionally stirred during 18h, all at 4°C. This solution was diluted with deionised water at a temperature of 20°C. The resulting solution served as stock solution with a measured analytical value of 66.7mg TOC/l resp. 75.0mg substance/l. 5 test concentrations and a control.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
clone 5, bred in house, test organisms <24 hours old. "0 organissma in 4 parallel sets of 5 (±1) each

Study design

Test type:
other: Closed, static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
None

Test conditions

Hardness:
250 mg/l CaCO3
Test temperature:
20°C ±1°C
pH:
7.7-8.2
Dissolved oxygen:
86-101 %
Salinity:
N/A
Nominal and measured concentrations:
nominal concentrations 3.3, 5.0, 6.7, 10.0 and 13.3mg.l-1. Geometric mean measured concentrations 2.8, 3.3, 4.5, 5.6 and 7.8mg.l-1
Details on test conditions:
To minimise the loss of the volatile test substance during the test, the volume inside the test vessels was increased to 20ml resulting in a reduced gas space. The vessels were closed tightly (gas tight septum).
Reference substance (positive control):
yes
Remarks:
potassium dichromate is tested as a reference substance quarterly

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.3 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 7.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Highest test concentration
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
5.6 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
As the nominal and measured concentrations deviated by more than 20% the effect concentration is reported as the geometric mean of the start and end concentrations.
Results with reference substance (positive control):
1mg K2Cr2O7 ≤50% effect.
2mg K2Cr2O7 ≥50% effect.
Reported statistics and error estimates:
No data reported

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Fulfills validity criteria
Conclusions:
The reported 48hr EC50 with buta-1,2-diene is 7.3mg/l
Executive summary:

The reported 48hr EC50 with buta-1,2-diene is 7.3mg/l. This study is GLP compliant and follows a standard guideline. In addition, the data required to assess validity has been included.