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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Repeated dose toxicity: inhalation

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Administrative data

Endpoint:
short-term repeated dose toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Referred in a recognized source of peer reviewed scientific data on chemicals

Data source

Reference
Reference Type:
publication
Title:
Subchronic Inhalation Studies of Styrene in CD Rats and CD-1 Mice.
Author:
Cruzan et al
Year:
1997
Bibliographic source:
Fundamental and Applied Toxicology Vol. 35: 152 -165

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 412 (Subacute Inhalation Toxicity: 28-Day Study)
Principles of method if other than guideline:
14-day Inhalation Repeated Toxicity Study
Study investigated: repeated dose subacute toxicity of styrene in mice
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Styrene
EC Number:
202-851-5
EC Name:
Styrene
Cas Number:
100-42-5
Molecular formula:
C8H8
IUPAC Name:
ethenylbenzene

Results and discussion

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

Study Observed:

 

Study in Mice:

Mortality/ Survival:

At ≥ 250 ppm (both sexes): mortality observed.

At ≥15 ppm (both sexes): signs of irritation.

At ≥ 250 ppm (both sexes): liver toxicity, including increased liver weights, livers had brown areas, irregular surface areas and adhesions. Microscopy revealed centrilobular hepatocyte necrosis and associated changes.

 

LOAEC (mice, inhalation, 2-week) =250 ppm [1065 mg/m3] (hepatotoxicity) (NOAEC =60 ppm [255 mg/m3])

Applicant's summary and conclusion

Conclusions:
The No Observed Adverse Effect concentrations for subacute inhalation exposure to styrene in mice are identified as follows:
LOAEC (mice, inhalation, 2-week) =250 ppm [1065 mg/m3] (hepatotoxicity) (NOAEC =60 ppm [255 mg/m3])