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Toxicological information

Repeated dose toxicity: inhalation

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Administrative data

Endpoint:
short-term repeated dose toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Referred in a recognized source of peer reviewed scientific data on chemicals

Data source

Reference
Reference Type:
publication
Title:
Subchronic Inhalation Studies of Styrene in CD Rats and CD-1 Mice.
Author:
Cruzan et al
Year:
1997
Bibliographic source:
Fundamental and Applied Toxicology Vol. 35: 152 -165

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 412 (Subacute Inhalation Toxicity: 28-Day Study)
Principles of method if other than guideline:
14-day Inhalation Repeated Toxicity Study
Study investigated: repeated dose subacute toxicity of styrene in mice
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

Study Observed:

 

Study in Mice:

Mortality/ Survival:

At ≥ 250 ppm (both sexes): mortality observed.

At ≥15 ppm (both sexes): signs of irritation.

At ≥ 250 ppm (both sexes): liver toxicity, including increased liver weights, livers had brown areas, irregular surface areas and adhesions. Microscopy revealed centrilobular hepatocyte necrosis and associated changes.

 

LOAEC (mice, inhalation, 2-week) =250 ppm [1065 mg/m3] (hepatotoxicity) (NOAEC =60 ppm [255 mg/m3])

Applicant's summary and conclusion

Conclusions:
The No Observed Adverse Effect concentrations for subacute inhalation exposure to styrene in mice are identified as follows:
LOAEC (mice, inhalation, 2-week) =250 ppm [1065 mg/m3] (hepatotoxicity) (NOAEC =60 ppm [255 mg/m3])